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The potential of phenanthrene to induce xenobiotic responses was investigated. Olive flounder (Paralichthys olivaceus) was exposed to different levels of phenanthrene $(0.5,\;1\;and\;{\mu}M)$ for 4 weeks, Gill CYP450 content and EROD (ethoxyresorufin O-deethylation) activity were found to...

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  • Test chambers (glass aquarium, 120 L capacity) were filled with 80 L of sea water. Water charac­ teristics, measured by the method described in APHA (1995), vere as follows; pH 8.
  • The phenanthrene dose was renewed every other day along with the seawater. Three test chambers were used for each treatment and each chamber contained 10 fish under semi-static conditions with airstones to maintain dissolved oxygen levels greater than 75% saturation.

이론/모형

  • Statistical analysis of the results was performed with the aid of SPSS/PC+ statistical package. ANOVAs and the Duncan's test for multiple compansons were used to test the significant differences between the control and treatment (Duncan, 1955).
  • cm" for A450-A490 (Omura and Sato, 1964). Gill EROD activity was determined using a modifi­ cation of the multiwell plate method of Kennedy and Jones (1994). The following reagent concentra­ tions were used in the EROD reaction mixture; 7-ethoxyresorufin, 1.
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참고문헌 (34)

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  15. Law, R.J., V.J. Dawes, R.J. Woodhead and P. Matthiessen. 1997. Polycyclic aromatic hydrocarbons (PAH) in seawater around England and Wales. Mar. Pollut. Bull., 34, 306-322 

  16. Lehr, R.E. and D.M. Jerina. 1977. Metabolic activation of polycyclic hydrocarbons. Arch. Toxicol., 39, 1-6 

  17. McElroy, A.E., J.W. Farrington and J.M. Teal. 1989. Bio availability of polycyclic aromatic hydrocarbons in the aquatic environment. In: Metabolism of Polycyclic Aromatic Hydrocarbons in the Aquatic Environment. Varamcsi, U., ed. CRC Press, Boca Raton, FL., pp. 1-40 

  18. Meador J.P., J.E. Stein, W.L. Reichert and U. Varanasi. 1995. A review of bioaccumulation of polycyclic aromatic hydrocarbons by marine organisms. Rev. Environ. Contam. Toxicol., 143, 79-165 

  19. Miller, M. R., D.E. Hinton and J.J. Stegeman. 1989. Cytochome P 450E induction and localization in gill pillar endothelial cells of scup and rainbow trout. Aquat. Toxicol., 14, 307-322 

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  33. Varanasi, U., J.E. Stein, W.L. Reichert, K.L. Tilbury, M.M. Krahn and S.L. Chan. 1992. Chlorinated and aromatic hydrocarbons in bottom sediments, fish and marine mammals in US coastal waters: laboratory and field studies of metabolism and accumulation. In: Persistent Pollutants in Marine Ecosystems. Walker, C.H. and D.R. Livingstone eds. Permagon Press, New York, pp. 83-115 

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