[논문]EFFECT OF TITANIUM SURFACE ROUGHNESS ON CELL ADHESION OF HUMAN OSTEOBLAST-LIKE CELLS (MG63)

Yim Soon-Ho

EFFECT OF TITANIUM SURFACE ROUGHNESS ON CELL ADHESION OF HUMAN OSTEOBLAST-LIKE CELLS (MG63) 원문보기

대한치과보철학회지 = The journal of Korean academy of prosthodontics, v.42 no.3, 2004년, pp.261 - 266

Yim Soon-Ho (Department of Prosthodontics, Samsung Medical Center, College of Medicine, Sungkyunkwan University)

Abstract ▼ AI-Helper

Statement of problem. The effects of surface roughness have not or insufficiently been analyzed on earlier events such as cell adhesion though cell behavior most germane to implant performance is cell adhesion. Purpose. The purpose of this study was to evaluate cell adhesion of osteoblast-like cells (MG63) onto three types of titanium disks with varying roughness using the Elisa assay. Materials and methods. Representative disks from each group (SLA, HA, machined) were subjected to surface analysis and surface roughness was measured by the optical interferometer (Accura 2000, Intekplus Co., Seoul, Korea). Following this, MG63 cells were cultured on the titanium disks and released. Cell adhesion measurements using the Elisa assay were performed specifically at three points: after 24, 48, and 72 hours of culture. Results. Among the 3 types of surface analyzed, the SLA surface was the roughest with a Ra value of $1.114{\mu}m$ followed by HA coated surface and machined surface, consecutively. The optical density values for the SLA surface group was significantly higher than that of the machined and HA coated surface groups following 24 and 48 hours of culture. The cell culture on HA coated surface showed significantly higher values compared to the machined surface following 24, 48 and 72 hours of culture. Conclusion. The results suggest that surface treatment of titanium surfaces enhanced cell adhesion of human osteoblast-like cells (MG63).

주제어

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가설 설정

1. The surface roughness of machind group was not different from that of HA coated group. (P>0.
4. Surface modifications using HAcoating and SLA can promote cellular attachments and enhance bone formation.

제안 방법

As the second part of this study, cell adhesion was measured following 24, 48 and 72 hours of culture. The control sample was calculated at the beginning of each cell culture.
is cell adhesion. The objective of this study was to evaluate cell adhesion of osteoblast like cells (MG63) onto three types of Titanium disks with varying roughness using the Elisa assay.
is cell adhesion. The objective of this study was to evaluate cell adhesion of osteoblast like cells (MG63) onto three types of Titanium disks with varying roughness using the Elisa assay.
The purpose of this study was to evaluate cell adhe sion of osteoblast-like cells (MG63) onto three types of titanium disks with varying roughness. Representative disks from each group (SLA, HA, machined) were subjected to surface analysis and surface roughness was measured by the optical interferometer (Accura 2000, Intekplus Cov Seoul, Korea).

대상 데이터

As a result, they are a good model for examining the early stages of osteoblastic differentiation. MG63 cells were obtained from the American Type Culture Collection (Rockvillz MD). Cells were plated at a concentration of 2.
MG63 osteoblast-like cells, originally isolated from a human osteosarcoma, were used in this study. This osteoblast-like cell line has been well-char acterized and contains numerous osteoblastic traits that are typical of a relatively immature osteoblast including high levels of 1Z25-(OH)2D3-responsive alkaline phosphatase and osteocalcin synthesis inhibition of proliferation when treat ed with 1, 25-(OH)2D3.
In order to increase the uniformity of the coat ing layer during the deposition process, sub strates were rotated at a speed of 8 rpm. The targets were made using a commercially available HA (Caio(P04)6(OH)2)powder (Alfa Aesar Co.z Ward Hill, MA, USA). Extra weight % of CaO powder (Cerac Co.

이론/모형

Cell adhesion onto each titanium disk was measured using the Elisa assay. 8 disks were used for each groups.
2ml and vortexing for 5 minutes, cells from the disk surface were released. Lysed cells were transferred to 96-well plate and the absorp tion optical density was calculated at 570nm using the Elisa assay.

성능/효과

3. Cell attachment of all three groups decreased following 72 hours of culture.
, Seoulz Korea). The results revealed that the Ra values of SLA surface were higher than that of HA coated and machined surfaces. Also, the Ra values of HA coated implants when compared to machined implants, showed only a marginal difference.
The optical density val ues for the SLA surface group was significantly higher than that of the machined and HA coated suface groups following 24 hours of culture. This was also true following 48 hours of culture as the SLA surface displayed significantly high er values. The cell culture on HA coated sur face showed significantly higher values com pared to the machined surface following 24z 48 and 72 hours of culture.

후속연구

This study con firmed that increasing surface roughness con tributes to adhesion of osteoblast-like cells (MG63). Although further studies seem necessary to strengthen this hypothesis, this finding presents a significant leap in understanding the early events of osteoblast-like cells on Titanium surfaces.

참고문헌 (19)

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