$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"

과학기술 지식인프라 ScienceON

상세검색 다국어 입력
도움말도움말
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

MyON담기
내보내기

Effective Extraction of Oligomeric Proanthocyanidin (OPC) from Wild Grape Seeds

Biotechnology and bioprocess engineering v.9 no.6 , 2004년, pp.471 - 475  
Huh Yun Suk (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology ) ;  Hong Tae Hee ( Department of Food & Nutrition, Daejeon Health Science College ) ;  Hong Won Hi ( Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
Abstract

The Oligomeric proanthocyanidin (OPC) in green and black tea, grape seeds, grapes and wine has raised much attention but that OPC in wild grape seed remains to be intensively investigated. This study investigated the total OPC contents and total antioxidant activity of wild grape seeds and developed an efficient extraction process with various temperatures, solvent compositions and times. Also, a chromatography column packed with the Dia-ion HP-20 resin was used for further purification of the ope. The total OPC contents were determined with the Folin-Ciocalteu reagent, and the antioxidant activity using total antioxidant potential (TAP) and 1,1-dipheny|-2picrylhydrazy| (DPPH). The yield of final purified OPC was 1.78 (+)-catechin equivalent (CE) g/100 g, with $IC_{50}$ activities of TAP and DPPH of 31.60 and $15.70\;{\mu}\;g/mL$. These activi­ties of the final purified OPC were about two times higher than that of the BHA used as a refer­ence sample.

주제어

#wild grape seed   #oligomeric proanthocyanidin (OPe)   #antioxidant activity   #DPPH  

저자의 다른 논문

허윤석(3) Hong, Won-Hi(27)

참고문헌 (20)

  1. de Gaulejac, C. T., Y. Glories, and N. Vivas (1999) Analysis of anthocaynidins in foods by liquid chromatography, liquid chromatography-mass spectrometry and capillary electrophoresis. J. Chromatogr. A 881: 327-333 
  2. Hagerman, A. E., K. M. Riedl, G. A. Jones, K. N. Sovik, N. T. Ritchard, P. W. Hartzfeld, and T. L. Riechel (1998) High molecular weight plant phenolics (tanins) as biological antioxidant. J. Agric. Food Chem. 46: 1887-1892. 
  3. Fuleki, T. J. and M. R. da Silva (1997) Catechin and proanthocyanidin composition of seeds from grape cultivars grown in Ontario. J. Agric. Food Chem. 45: 1156-1160 
  4. Frankel, E. N., A. L. Waterhouse, and P. L. Teissedre (1995) Principal phenolic phytochemicals in selected California wines and their antioxidant activity in inhibiting oxidation of human low-density lipoproteins. J. Agric. Food Chem. 43: 890-894 
  5. Campodonico, P., E. Barbieri, M. Pizarro, C. P. Sotomayor, and E. A. Lissi (1998) A comparison between total phenol content of wines and their TRAP values measured by the bleaching of ABTS radical cations. B. Soc. Chil. Quim. 43: 281-285 
  6. Chen, C. W. and C. T. Ho (1995) Antioxidant properties of poly phenols extracted from green and black tea. J. Food Lipids 2: 35-46 
  7. Singleton, V. L., R. Orthofer, and R. M Lamuela-Raventos (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Method Enzymol. 299: 152-178. 
  8. Catapano, A. L. (1997) Antioxidant effect of flavonoids. Angiology 48: 39-44 
  9. Kim, M. A. and J. K. Park (2002) High frequency plant regeneration of garlic (Allium sativum L.) calli immobilized in calcium alniante gel. Biotechnol. Bioprocess Eng. 7: 206-211 
  10. Prakash, G., S. S. Bhojwani, and A. K. Srivastava (2002) Production of azadirachtin from plant tissue culture: State of the art and future prospects. Biotechnol. Bioprocess Eng. 7: 185-193 
  11. Sun, B., C. Leandro, J. R. da Silva, and I. Spranger (1998) Separation of grape and wine proanthocyanidins according to their degree of polymerization. J. Agric. Food Chem. 46: 1390-1396 
  12. Bagchi, D., R. L. Krohn, A. Garg, J. Balmoori, M. Bagchi, D. J. Bagchi, M. X. Tran, and S. J. Stohs (1997) Comparative in vitro and in vivo free radical scavenging abilities of grape seed proanthocyanidins and selected antioxidants. FASEB J. Abstr. 11: 3369. 
  13. Pekic, B., V. Kovac, E. Alonso, and E. Revilla (1998) Study of the extraction of proanthocyanidins from grape seeds. Food Chem. 61: 201-206 
  14. Chen, C. C., L. L. Shi, and C. C. Chen (1996) Effect of extraction temperature and time on polyphenol contents and composition and sensory quality of oolong tea infusion. Food Sci. Taiwan 23: 285-298 
  15. de Freitas, V. A. P., Y. Glories, G. Bourgeois, and C. Vitry (1998) Characterization of oligomeric and polymeric proanthocyanidins from grape seeds by liquid second ion mass spectrometry. Phytochemistry 49: 1435-1441. 
  16. Bravo, L. (1998) Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr. Rev. 56: 317- 333 
  17. Nonaka, G. I., N. Miwa, and I. Nishioka (1982) Stilbene glycoside gallates and proanthocyanidins from polygomum multiflorum. Phytochemistry 21: 429-432 
  18. Santos-Buelga, C., E. M. Francia-Aricha, and M. T. Escribano-Bailon (1995) Comparative falvan-3-ol composition of seeds from different grape varieties. Food Chem. 53: 197-201 
  19. Robinson, E. E., S. R. J. Maxwell, and G. H. G. Thorpe (1997) An investigation of the antioxidant activity of black tea using enhanced chemiluminescence. Free Radical Res. 26: 291-302 
  20. Yamakoshi, J., M. Satio, S. Kataoka, and M. Kikuchi (2002) Safety evaluation of proanthocyanidin-rich extract from grape seeds. Food Chem. Toxicol. 40: 599-607 

이 논문을 인용한 문헌 (1)

  1. 2009. "" Biotechnology and bioprocess engineering, 14(3): 345~348 

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일