[논문]Effective Extraction of Oligomeric Proanthocyanidin (OPC) from Wild Grape Seeds

Huh Yun Suk; Hong Tae Hee; Hong Won Hi

doi:10.1007/bf02933488

Effective Extraction of Oligomeric Proanthocyanidin (OPC) from Wild Grape Seeds 원문보기

Biotechnology and bioprocess engineering : Bbe, v.9 no.6, 2004년, pp.471 - 475

Huh Yun Suk (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) , Hong Tae Hee (Department of Food & Nutrition, Daejeon Health Science College) , Hong Won Hi (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)

Abstract ▼ AI-Helper

The Oligomeric proanthocyanidin (OPC) in green and black tea, grape seeds, grapes and wine has raised much attention but that OPC in wild grape seed remains to be intensively investigated. This study investigated the total OPC contents and total antioxidant activity of wild grape seeds and developed an efficient extraction process with various temperatures, solvent compositions and times. Also, a chromatography column packed with the Dia-ion HP-20 resin was used for further purification of the ope. The total OPC contents were determined with the Folin-Ciocalteu reagent, and the antioxidant activity using total antioxidant potential (TAP) and 1,1-dipheny|-2picrylhydrazy| (DPPH). The yield of final purified OPC was 1.78 (+)-catechin equivalent (CE) g/100 g, with $IC_{50}$ activities of TAP and DPPH of 31.60 and $15.70\;{\mu}\;g/mL$. These activities of the final purified OPC were about two times higher than that of the BHA used as a reference sample.

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Effects of Temperature, Solvent Composition and Time on OPCS Extraction OPC are known to be sensitive to extraction conditions, such as temperature, solvent composition and time [18]. This work investigated the total OPC contents and total antioxidant activity of wild grape seeds and developed an efficient extraction process, with various temperatures, solvent compositions and times.

대상 데이터

The WGS was provided by Daejeon Health Science College (Daejeon, Korea). The OPC were extracted from the WGS (200 g) with various ratios of ethanol/water (800 mL), and at various temperatures (50~90℃) and times (1~12 h) in 2-L glass vessels with stirring by 200 rpm.

참고문헌 (20)

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