[논문]An Animal Model to Evaluate the Protective Efficacy of Haemophilus influenzae Type b Conjugate Vaccines

Kim Hyun Sung; Yoo Tae Hyeon; Jang Yang Suk; Kim Hun; Park Jin Yong; Hur Byung Ki; Ryu Yeon Woo; Kim Jong Su

doi:10.1007/bf02933491

An Animal Model to Evaluate the Protective Efficacy of Haemophilus influenzae Type b Conjugate Vaccines 원문보기

Biotechnology and bioprocess engineering : Bbe, v.9 no.6, 2004년, pp.490 - 494

Kim Hyun Sung (GreenCross Vaccine Corporation) , Yoo Tae Hyeon (GreenCross Vaccine Corporation) , Jang Yang Suk (GreenCross Vaccine Corporation) , Kim Hun (GreenCross Vaccine Corporation) , Park Jin Yong (GreenCross Vaccine Corporation) , Hur Byung Ki (Department of Biotechnology and Bioengineering, Inha University) , Ryu Yeon Woo (Department of Molecular Science and Technology, Ajou University) , Kim Jong Su (GreenCross Vaccine Corporation)

Abstract ▼ AI-Helper

An efficacy test of PRP (polyribosylribitol phosphate)-TT (Tetanus toxoid) conjugate vaccines was carried out using BALB/c mice as an animal model by inoculating Haemophilus influenzae type b (Hib) with a virulence enhancement factor (VEF). Three administrations of the conjugate vaccines at 2-week intervals elicited a significantly high level of PRP antibodies (P>0.0001). The protective activity of the PRP immunization was challenged with either Hib with iron dextran (Hib/) or with a combination of mucin and hemoglobin (Hibmh) as a VEF. The medium lethal dose $(LD_{50})$ for Hibmh and Hibiwas measured as 10 CFU (Colony Forming Unit) and $2.5{\times}10^{8}$ CFU respectively. Each immunized animal was challenged with five or ten times the $LD_{50}$ level of bacteria with a VEF. A significant difference in mortality between the immunized and control mice (P> 0.01) was observed with the Hibmh challenge inoculation but not with the Hibi challenge inoculation. These results show that a combination of mucin and hemoglobin was able to enhance the virulence of Hib in BALB/c mice to cause a lethal infection, thus suggesting that BALB/c mice introduced to this method can be an effective model animal for testing the protective efficacy of H. influenzae conjugate vaccines.

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문제 정의

This study shows that the protective efficacy of PRP- TT conjugate vaccines can be evaluated by challenging immunized BALB/c mice with Hib with a combination of mucin and hemoglobin. The animal model proposed in this study does not require other kinds of analyses (e.

제안 방법

However, the protective efficacy evaluation of Hib conjugate vaccines using animal models has not been reported. In this study, we explored the effectiveness of a BALB/c mice model challenged by Hib with a virulence enhancement factor for testing the efficacy of Hib conjugate vaccines. Iron dextran and a combination of mucin and hemoglobin were selected based on previously published results [15, 1 기.

데이터처리

PRP antibody titers were measured by ELISA [18] and the results are shown in Table 1. A statistical comparison of antibody levels was done by the student ^-test analysis. The injection of PRP-TT conjugate vaccines elicited a statistically significant rise of PRP antibodies (P < 0.

이론/모형

The LD50, the dose that causes 50% mortality was estimated by the probit method [21].

성능/효과

However, the results from the different strains showed an insignificant difference between these virulence enhancements. In this study, the infection sizes were determined to be 5 and 10 times that of LD50, respectively (Table 3).

후속연구

This test method may be expanded to evaluate other types of Hib conjugate vaccines, e.g. PRP-CRM197, PRP-DT and PRP-OMP. Additional data are needed to establish the degree of correlation between human antibody responses and the prevention of bacteremia in the current animal model.

참고문헌 (28)

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원문보기 상세보기
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