[논문]Purification and Properties of Intracellular Cytosine Deaminase from Chromobacterium violaceum YK 391

KIM , JUNG; YU, TAE-SHICK

Purification and Properties of Intracellular Cytosine Deaminase from Chromobacterium violaceum YK 391 원문보기

Journal of microbiology and biotechnology, v.14 no.6, 2004년, pp.1182 - 1189

KIM , JUNG (Department of Dental Hygiene, Suwon Womens College) , YU, TAE-SHICK (Department of Microbiology, Keimyung University)

Abstract ▼ AI-Helper

Cytosine deaminase (cytosine aminohydrolase, EC 3.5.4.1) stoichiometrically catalyzes the hydrolytic deamination of cytosine and 5-fluorocytosine to uracil and 5-fluorouracil, respectively. The intracellular cytosine deaminase from Chromobacterium violaceum YK 391 was purified to apparent homogeneity with 272.9-fold purification with an overall yield of $13.8\%$. The enzyme consisted of dimeric polypeptides of 63 kDa, and the total molecular mass was calculated to be approximately 126 kDa. Besides cytosine, the enzyme deaminated 5-fluorocytosine, cytidine, 6-azacytosine, and 5-methylcytosine, but not 5-azacytosine. Optimum pH and temperature for the enzyme reaction were 7.5 and $30^{\circ}C$, respectively. The enzyme was stable at pH 6.0 to 8.0, and at 30T for a week. About $70\%$ of the enzyme activity was retained at $60^{\circ}C$ for 5 min. The apparent $K_{m}$ values for cytosine, 5-fluorocytosine, and 5-methylcytosine were calculated to be 0.38 mM, 0.87 mM, and 2.32 mM, respectively. The enzyme activity was strongly inhibited by 1 mM $Hg^{2+},\;Zn^{2+},\;Cu^{2+},\;Pb^{2+},\;and\;Fe^{3+}$, and by o-phenanthroline, $\alpha,\;{\alpha}'$-dipyridyl, p-choromercuribenzoate, N-bromosuccinimide, and cWoramineT. In addition, the enzyme activity was strongly inhibited by I mM 2-thiouracil, and weakly inhibited by 2-thiocytosine, or 5-azacytosine. Finally, intracellular and extracellular cytosine deaminases from Chromobacterium violaceum YK 391 were found to have a different optimum temperature, apparent $K_{m}$ value, and molecular mass.

주제어

참고문헌 (32)

Andrews, P. 1964. Estimation of the molecular weights of proteins by Sephadex gel filtration. Biochem. J. 91: 222- 233
Bradford, B. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing for the principle of protein-dye binding. Anal. Biochem. 72: 248-251

상세보기
Davis, B. J. 1964. Disc electrophoresis. II. Method and application to human serum protein gel electrophoresis conference chairman. Fredrich, J. F. Ann. New York Acad. Sci. 121: 404-427
Duschinsky, R., E. Pleven, and C. Heiderberger. 1957. The synthesis of 5-fluoropyrimidines. J. Am. Chem. Soc. 79: 4559-4560
Giege, R. and J. H. Weil. 1970. Elude des tRNA de levureagent incopore du 5-fluorouracile provenant de la desamination in vivo de la 5-fluorocytosine. Bull. Soc. Chim. Biol. 52: 135-144
Grunberg, E., E. Titsworth, and M. Bennett. 1964. Chemotherapeutic activity of 5-fluorocytosine, pp. 566- 568. In: Antimicrobial Agents and Chemotherapy. Ann Arbor. U.S.A
Hahn, A. and W. Lintzel. 1923. Uer das verhalten von pyrimidinderivaten in den organismen. I. Einfluss von hefe auf pyrimidinderivate. Z. Biol. 79: 179-184
Hahn, A. and L. Schafer. 1925. Uer das verhalten von pyrimidin derivaten in den organismen. II. Einwirkung von Bacterium coli auf uracil und cytosin. Z. Biol. 83: 511-514
Ipata, P. L., F. Marmochii, G. Magni, R. Felio, and G. Polidoro. 1971. Baker’s yeast cytosine deaminase. Some enzymatic properties and allosteric inhibition by nucleosides and nucleotides. Biochemistry 10: 4270-4276
Katsuragi, T., T. Sakai, K. Matsumoto, and K. Totonomura. 1986. Cytosine deaminase from E. coli-production, purification and some characteristics. Agric. Biol. Chem. 50: 1721-1730
Katsuragi, T., T. Sakai, and K. Tonomura. 1987. Implantable enzyme capsules for cancer chemotherapy from Bakers’ yeast cytosine deaminase immobilized on epoxy-acrylic resin and urethane prepolymer. Appl. Biochem. Biotechnol. 16: 61-69
Katsuragi, T., T. Sonoda, K. Matsumoto, T. Sakai, and K. Totonomura. 1989. Purification and some properties cytosine deaminase from Bakers’ yeast. Agric. Biol. Chem. 53: 1313-1319
Kim, J., H. S. Kim, and T. S. Yu. 2002. Optimal conditions for the production of intracellular cytosine deaminase from Chromobacterium violaceum YK 391. Kor. J. Appl. Microbiol. Biotechnol. 30: 367-372
Koechlin, B. A., F. Rubio, S. Palmer, T. Gabriel, and R. Duschinsky. 1966. The metabolism of 5-fluorocytosine- 214C and cytosine-14C in the rat and the disposition of 5-fluorocytosine-214C in man. Biochem. Phamacol. 15: 435-446
Kono, A., Y. Hara, and Y. Matsushima. 1981. Enzymatic formation of 5-fluorouracil from 1-(tetrahydro-2-furanyl)-5- fluorouracil (tegafur) in human tumor tissue. Chem. Pharm. Bull. 29: 1486-1488
Kono, A., Y. Hara, S. Sugata, Y. Karube, Y. Matsushima, and H. Ishitsuka. 1983. Activation of 5'-deoxy-5-fluorouridine by thymidine phosphorylase in human tumors. Chem. Pharm. Bull. 31: 175-178
Kream, J. and E. Chargaff. 1952. On the cytosine deaminase of yeast. J. Amer. Chem. Soc. 73: 5157-5150
Lineweaver, H. and D. Burk. 1934. The determination of enzyme dissociation constants. J. Amer. Chem. Soc. 56: 658-666

상세보기
Nakanishi, Y., F. Isohaschi, S. Ebisumo, and Y. Sakamoto. 1989. Sulfhydryl groups of an extramitochondrial acetyl CoA hydrolase from rat liver. Biochim. Biophys. Acta 996: 209-213
Nishiyama, T., Y. Kawamura, K. Kwamoto, H. Matsumura, N. Yamamoto, T. Ito, A. Ohyama, T. Katsaragi, and T. Sakai. 1982. Antineoplastic effect of 5-fluorocytosine and cytosine deaminase on brain tumor. Neurol. Med. Chirs. 22: 344- 352
Sakai, T., T. S. Yu, H. Tabe, and S. Omata. 1975. Purification of cytosine deaminase from Serratia marcescens. Agr. Biol. Chem. 39: 1623-1629
Sakai, T., T. S. Yu, K. Taniguchi, and S. Omata. 1975. Purification of cytosine deaminase from Pseudomonas aureofaciens. Agr. Biol. Chem. 39: 2015-2020
Segel, I. H. 1976. Biochemical Calculations (2nd Ed.), New York, John Wiley and Sons, Inc. p. 203
Wallace, P. M., J. F. MacMaster, V. F. Smith, D. E. Kerr, P. D. Senter, and W. L. Cosand. 1994. Intratumoral generation of 5-fluorouracil mediated by an antibody-cytosine deaminase conjugate in combination with 5-fluorocytosine. Cancer Res. 54: 2719-2723
Weber, A. K. and M. Osborn. 1969. The reliability of molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis. J. Biol. Chem. 244: 4406-4412
West, T. M., S. Shanly, and G. A. O'Do novan. 1982. Purification and some properties of cytosine deaminase from Salmonella typhimurium. Biochim. Biophys. Acta 719: 251- 258
Yeeh, Y., J. H. Park, and H. K. Jun. 1985. Properties of extracellular cytosine deaminase from Arthrobacter sp. JH-13. Kor. J. Microbiol. 23: 177-183
Yu, T. S. and T. H. Kim. 1999. Purification and properties of extracellular cytosine deaminase from Chromobacterium violaceum YK 391. J. Microbiol. Biotechnol. 9: 173-178
Yu, T. S., T. H. Kim, J. M. Park, H. I. Song, and K. T. Chung. 1988. Optimum culture conditions for production of extracellular cytosine deaminase from Bacillus polymyxa YL 38-3. Kor. J. Microbiol. 26: 368-374
Yu, T. S., J. K. Kim, T. Katsuragi, T. Sakai, and K. Tonomura. 1991. Purification and some properties of cytosine deaminase from Aspergillus fumigatus. J. Ferment. Bioeng. 72: 266-269
Yu, T. S., T. Sakai, and S. Omata. 1976. Kinetic properties of cytosine deaminase from Pseudomonas aureofaciens. Agr. Biol. Chem. 40: 551-557
Yu, T. S., J. K. Kim, and T. Saki. 1986. Cytosine deaminase of fungus. Kor. J. Appl. Microbiol. Biotechnol. 14: 169-174

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증