$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Growth Temperature-Dependent Conversion of De novo-Synthesized Unsaturated Fatty Acids into Polyhydroxyalkanoic Acid and Membrane Cyclopropane Fatty Acids in the Psychrotrophic Bacterium Pseudomonas fluorescens BM07

Abstract

A psychrotrophic bacterial strain, Pseudomonas fluorescens BM07, synthesized unsaturated fatty acids (UFA) from fructose in response to lowering of growth temperature, and incorporated them into both polyhydroxyalkanoic acid (PHA) and membrane lipid. The blocking of PHA synthesis by adding 5 mM 2-bromooctanoic acid to the growth medium, containing 70 mM fructose, was found to be a useful means to profile the composition of membrane lipid by gas chromatography. As the growth temperature changed from 35 to $50^{\circ}C$, the total content of two UFA, 3-hydroxy-cis-5­dodecenoic acid ($C_{12:1}$) and 3-hydroxy-cis-7-tetradecenoic acid ($C_{14:1}$), in PHA increased from 31 to 44 $mol\%$. The growth at lower temperatures also led to an increase in the level of two major UFA, palmitoleic acid (C16:1 cis9) and cis-vaccenic acid (C18:1 cis11), in membrane lipid. A fraction of these membrane-lipid UFA was converted to their corresponding cyclopropane fatty acids (CFA). The CFA conversion was a function of culture time, exhibiting biphasic increase before and after entering the stationary phase. However, pH changes in growth media had no effect on the CFA conversion, which is contrary to the case of E. coli reported. The cells grown at $30^{\circ}C$ responded to a cold shock (lowering the medium temperature down to $10^{\circ}C$) by increasing the level of C16:1 cis9 and C 18: I cis II up to that of $10^{\circ}C$-grown control cells and concomitantly decreasing the relative level of cis-9,10­methylenehexadecanoic acid (the CFA converted from C16:1 cis9) from 14 to 8 $mol\%$, whereas the 10-grown cells exhibited little change in the lipid composition when exposed to a warmer environment of $30^{\circ}C$ for 12 h. Based on this one- way response, we suggest that this psychrotrophic strain responds more efficiently and sensitively to a cold shock than to a hot shock. It is also suggested that BM07 strain is a good producer of two unsaturated 3-hydroxyacids, $C_{12:1}\;and\;C_{141:1}$.

참고문헌 (20)

  1. Alvarez, H. M., O. H. Pucci, and A. Steinbüchel. 1997. Lipid storage compounds in marine bacteria. Appl. Microbiol. Biotechnol. 47: 132-139 
  2. de Waard, P., H. van der Wal, G. N. M. Huijberts, and G. Eggink. 1993. Heteronuclear NMR analysis of unsaturated fatty acids in poly(3- hydroxyalkanoates). J. Biol. Chem. 268: 315-319 
  3. Sundheim, G., A. Sletten, and R. H. Dainty. 1998. Identification of pseudomonads from fresh and chill-stored chicken carcasses. Int. J. Food Microbiol. 39: 185-194 
  4. Dionisi, F., P.-A. Golay, M. Elli, and L. B. Fay. 1999. Stability of cyclopropane and conjugated linoleic acids during fatty acid quantification in lactic acid bacteria. Lipids 34: 1107-1115 
  5. Rehm, B. H. A., N. Kroger, and A. Steinbüchel. 1998. A new metabolic link between fatty acid synthesis and polyhydroxyalkanoic acid synthesis. J. Biol. Chem. 273: 24044-24051 
  6. Chang, Y.-Y., J. Eichel, and J. E. Cronan, Jr. 2000. Metabolic instability of Escherichia coli cyclopropane fatty acid synthase is due to RpoH-dependent proteolysis. J. Bacteriol. 182: 4288-4294 
  7. Huijberts, G. N. M., G. Eggink, P. de Waard, G. W. Huisman, and B. Witholt. 1992. Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers. Appl. Environ. Microbiol. 58: 536-544 
  8. Guillou, C. and J. F. Guespin-Michel. 1996. Evidence for two domains of growth temperature for the psychrotrophic bacterium Pseudomonas fluorescens MF0. Appl. Environ. Microbiol. 62: 3319-3324 
  9. Grogan, D. W. and J. E. Cronan, Jr. 1997. Cyclopropane ring formation in membrane lipids of bacteria. Microbiol. Mol. Biol. Rev. 61: 429-441 
  10. Sudesh, K., K. Taguchi, and Y. Doi. 2002. Effect of increased PHA synthase activity on polyhydroxyalkanoates biosynthesis in Synechocystis sp. PCC6803. Int. J. Biol. Macromol. 30: 97-104 
  11. Chang, Y.-Y. and J. E. Cronan, Jr. 1997. Membrane cyclopropane fatty acid content is a major factor in acid resistance of Escherichia coli. Mol. Microbiol. 33: 249-259 
  12. Lee, H.-J., M. H. Choi, T.-U. Kim, and S. C. Yoon. 2001. Accumulation of polyhydroxyalkanoic acid containing large amounts of unsaturated monomers in Pseudomonas fluorescens BM07 utilizing saccharides and its inhibition by 2-bromooctanoic acid. Appl. Environ. Microbiol. 67: 4963- 4974 
  13. Kim, J. S., H. Y. Weon, S. W. Kwon, and J. C. Ryu. 2003. Bacterial community variations in hot pepper-sown soil using FAME analysis as an indicator of soil quality. J. Microbiol. Biotechnol. 13: 251-255 
  14. Choi, M. H. and S. C. Yoon. 1994. Polyester biosynthesis characteristics of Pseudomonas citronellolis grown on various carbon sources, including 3-methyl-branched substrates. Appl. Environ. Microbiol. 60: 3245-3254 
  15. Kim, J. S., J. B. Joo, H. Y. Weon, C. S. Kang, S.-K. Lee, and C. S. Yahng. 2002. FAME analysis to monitor impact of organic matter on soil bacterial populations. J. Microbiol. Biotechnol. 12: 382-388 
  16. Magnuson, K., S. Jackowski, C. O. Rock, and J. E. Cronan, Jr. 1993. Regulation of fatty acid biosynthesis in Escherichia coli. Microbiol. Rev. 57: 522-542 
  17. Beney, L. and P. Gervais. 2001. Influence of the fluidity of the membrane on the response of microorganisms to environmental stresses. Appl. Microbiol. Biotechnol. 57: 34-42 
  18. Raaka, B. M. and J. M. Lowenstein. 1979. Inhibition of fatty acid oxidation by 2-bromooctanoate. J. Biol. Chem. 254: 6755-6762 
  19. Fritzsche, K., R. W. Lenz, and R. C. Fuller. 1990. Production of unsaturated polyesters by Pseudomonas oleovorans. Int. J. Biol. Macromol. 12: 85-91 
  20. Shokri, A., A. M. Sandén, and G. Larsson. 2002. Growth rate-dependent changes in Escherichia coli membrane structure and protein leakage. Appl. Microbiol. Biotechnol. 58: 386-392 

이 논문을 인용한 문헌 (2)

  1. 2007. "" Journal of microbiology and biotechnology, 17(2): 373~377 
  2. Kim, Hyun-Do ; Choi, Jong-Il 2014. "Effect of Temperature on Growth Rate and Protease Activity of Antarctic Microorganisms" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 42(3): 293~296 

원문보기

원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일