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Secretory Production of Rahnella aquatilis ATCC 33071 Levansucrase Expressed in Escherichia coli 원문보기

Journal of microbiology and biotechnology, v.14 no.6, 2004년, pp.1232 - 1238  

KANG , SOON-AH (Department of Molecular Biotechnology, Bio) ,  LEE, JAE-CHEOL (Department of Food and Nutrition, Samcheok National University) ,  PARK, YOUNG-MIN (Department of Dental Hygiene, YeoJoo Institute of Technology) ,  LEE, CHAN (Department of Food and Biotechnology, Hanseo University) ,  KIM, SEUNG-HWAN (Korea Food and Drug Administration) ,  CHANG, BYUNG-IL (RealBiotech Co. Ltd.) ,  KIM, CHUL-HO (Biotechnology Research Division, KRIBB) ,  SEO, JEONG-WOO (Biotechnology Research Division, KRIBB) ,  RHEE, SANG-KI (Biotechnology Research Division, KRIBB) ,  JUNG, SUNG-JE (Department of Food Science and Technology, Kyung Hee University) ,  KIM, SANG-MOO (Faculty of Marine Bioscience and Technology, Kangnung National University) ,  PARK, SEONG-KYU (Department of Prescriptionary, College of Oriental Medicine) ,  JANG, KI-HYO (Department of Food and Nutrition, Samcheok National University)

Abstract AI-Helper 아이콘AI-Helper

To investigate the production and characteristics of thermostable levan sucrase from Rahnella aquatilis ATCC 33071, the levan sucrase gene from R. aquatilis was cloned and expressed in Escherichia coli without induction system. Expression of levansucrase gene in E. coli had no notable or detrimental...

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참고문헌 (34)

  1. Blight, M. A., C. Chervaux, and I. B. Holland. 1994. Protein secretion pathways in Escherichia coli. Curr. Opin. Biotech. 5: 468-474 

  2. Bradford, M. M. 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein of protein-dye binding. Anal. Biochem. 72: 248-254 

  3. Calazans, G. M. T., R. C. Lima, F. P. Francisca, and C. E. Lopes. 2000. Molecular weight and antitumor activity of Zymomonas mobilis. Int. J. Biol. Macromol. 27: 245-247 

  4. Chalmers, J. J., E. Kim, J. N. Telford, E. Y. Wong, W. C. Tacon, M. L. Shuler, and D. B. Wilson. 1990. Effects of temperature on Escherichia coli overproducing $\beta$ -lactamase or human epidermal growth factor. Appl. Environ. Microbiol. 56: 104-111 

  5. Chun U. H. and P. L. Rogers. 1988. The simultaneous production of sorbitol from fructose and gluconic acid from glucose using an oxidoreductase of Zymomonas mobilis. App. Microbiol. Biotechnol. 29: 19-24 

  6. Goebel, W. and J. Hedgpeth. 1982. Cloning and functional characterization of the plasmid-encoded hemolysin determinant of Escherichia coli. J. Bacteriol. 151: 290-298 

  7. Hammes, W., K. H. Scheifer, and O. Kandler. 1973. Mode of action of glycine on the biosynthesis of peptidoglycan. J. Bacteriol. 116: 1029-1053 

  8. Han Y. W. 1990. Microbial levan. Adv. Appl. Microbiol. 35: 171-194 

  9. Hendry, G. A. F. and R. K. Wallace. 1993. The origin, distribution, and evolutionary significance of fructans. In M. Suzuki and N. J. Chatterton (eds.), Science and Technology of Fructans. CRC Press 

  10. Ikura, Y. 1986. Effect of glycine and its derivatives on production and release of beta-galactosidase by Escherichia coli. Agric. Biol. Chem. 50: 2747-2753 

  11. Jang, E. K., K. H. Jang, I. Koh, I. H. Kim, S. H. Kim, S. A. Kang, C. H. Kim, S. D. Ha, and S. K. Rhee. 2002. Molecular characterization of the levansucrase gene from Pseudomonas aurantiaca S-4380 and its expression in Escherchia coli. J. Microbiol. Biotechnol. 12: 603-609 

  12. Jang, K. H., J. W. Seo, K. B. Song, C. H. Kim, and S. K. Rhee. 1999. Extracellular secretion of levansucrase from Zymomonas mobilis in Escherichia coli. Bioprocess Eng. 21: 453-458 

  13. Jang, K. H., K. B. Song, C. H. Kim, B. H. Chung, S. A. Kang, U. H. Chun, R. W. Choue, and S. K. Rhee. 2001. Comparison of characteristics of levan produced by different preparations of levansucrase from Zymomonas mobilis. Biotechnol. Lett. 23: 339-344 

  14. John E. Van W. and A. D. Johannis. 1990. How big is the periplasmic space? Trends Biochem. Sci. 15: 136-137 

  15. Kim, H. J., H. E. Park, M. J. Kim, H. G. Lee, J. Y. Yang, and J. H. Cha. 2003. Enzymatic characterization of a recombinant levansucrase from Rahnella aquatilis ATCC 15552. J. Microbiol. Biotechnol. 13: 230-235 

  16. Kim, S. H., E. K. Jang, I. H. Kim, K. H. Jang, S. A. Kang, and B. I. Chang. 2003. Effect of glycine supplement on extracellular secretion of levansucrase from seudomonas aurantiaca S-4380 in recombinant Escherichia coli. Korean J. Biotechnol. Bioeng. 18: 312-317 

  17. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 

  18. Leibovici J., S. Kopel, A. Siegel, and O. Gal-Mor. 1986. Effect of tumor inhibitory and stimulatory doses of levan, alone and in combination with cyclophosphamide, on spleen and lymph nodes. Int. J. Immunopharmacol. 8: 391-403 

  19. Loo, J. V., J. Cummings, N. Delzenne, H. Englyst, A. Frank, M. Hopkins, N. Kok, G. Macfalane, D. Newton, M. Quigley, M. Roberfroid, T. van Vliet, and E. van den Heuvel. 1999. Functional food properties of non-digestible oligosaccharides: A consensus report from the ENDO project (DGXII AIRIICT94- 1095). Br. J. Nutr. 81: 121-132 

  20. Lyness, E. W. and H. W. Doelle. 1983. Levansucrase from Zymomonas mobilis. Biotechnol. Lett. 5: 345-350 

  21. Ohtsuka, K., S. Hino, T. Fukushima, O. Ozawa, T. Kanematsu, and T. Uchida. 1992. Characterization of levansucrase from Rahnella aquatilis JCM-1683. Biosci. Biotech. Biochem. 56: 1373-1377 

  22. Osborn, M. J., J. E. Gander, E. Parisi, and J. Carson. 1972. Mechanism of assembly of the outer membrane of Salmonella typhimurium; isolation and characterization of cytoplasmic and outer membranes. J. Biol. Chem. 247: 3962-3972 

  23. Patil, N. K., U. Sharanagouda, J. H. Niazi, C. K. Kim, and T. B. Karegoudar. 2003. Degradation of salicylic acid by free and immobilized cells of Pseudomonas sp. strain NGK1. J. Microbiol. Biotechnol. 13: 29-34 

  24. Pelicic, V., J. M. Reyrat, and B. Gicquel. 1996. Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria. J. Bacteriol. 178: 1197-1199 

  25. Rhee, S. K., K. B. Song, C. H. Kim, B. S. Park, E. K. Jang, and K. H. Jang. 2002. Levan, pp 351-377. In S. Alexander, E. Vandamme, and S. De. Baets (eds.), Biopolymers, vol. 5. Wiley-VCH, Weinheim, Germany 

  26. Salmond, G. P. C. and P. J. Reeves. 1993. Membrane traffic wardens and protein secretion in Gram-negative bacteria. Trends Biochem. Sci. 18: 7-12 

  27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A 

  28. Seo, J. W., K. B. Song, K. H. Jang, C. H. Kim, B. H. Jung, and S. K. Rhee. 2000. Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli. J. Biotechnol. 81: 63-72 

  29. Seo, J. W., K. H. Jang, S. A. Kang, K. B. Song, E. K. Jang, B. S. Park, C. H. Kim, and S. K. Rhee. 2002. Molecular cloning of the growth phase-dependent expression of the lsrA gene, encoding levansucrase of Rahnella aquatilis. J. Bacteriol. 184: 5862-5870 

  30. Singh, Y. 2003. Photosynthetic activity, and lipid and hydrocarbon production by alginate immobilized cells of Botrycoccus in relation to growth phase. J. Microbiol. Biotechnol. 13: 687-691 

  31. Song, K. B., H. Belghith, and S. K. Rhee. 1996. Production of levan, a fructose polymer, using an overexpressed recombinant levansucrase. Ann. N. Y. Acad. Sci. 799: 601- 607 

  32. Sunita, K., B. H. Chung, K. H. Jang, K. B. Song, C. H. Kim, and S. K. Rhee. 2000. Refolding and purification of Zymomonas mobilis levansucrase produced as inclusion bodies in fed-batch culture of recombinant Escherichia coli. Protein Expres. Purif. 18: 388-393 

  33. Tanaka, T., S. Oi, and T. Yamamoto. 1979. Synthesis of levan by levansucrase. J. Biochem. 85: 287-293 

  34. Yanase, H., M. Iwata, R. Nakahigashi, K. Kita, N. Kato, and K. Tonomura. 1992. Purification, crystallization and properties of the extracellular levansucrase from Zymomonas mobilis. Biosci. Biotech. Biochem. 56: 1335-1337 

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