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Abstract

To investigate the production and characteristics of thermostable levan sucrase from Rahnella aquatilis ATCC 33071, the levan sucrase gene from R. aquatilis was cloned and expressed in Escherichia coli without induction system. Expression of levansucrase gene in E. coli had no notable or detrimental effect on the growth of host strain, and the recombinant levan sucrase exhibited levan synthesis activity. Levansucrase was secreted to the periplasm in E. coli, and addition of $0.5\%$ glycine yielded further secretion of levansucrase to the growth medium and resulted in an increase of total levansucrase activity. Furthermore, the cellular levansucrase was evaluated for the production of levan by using toluene­permeabilized whole-cells. The levansucrase was thermostable at $37^{\circ}C$. The molecular size oflevan was $1{\times}\;10^{6}$ Da, as determined by HPLC, and the degree of polymerization of levan varied with incubation temperatures: Low incubation temperature was preferable for the production of high-molecular size levan. The present study demonstrated that the mass production of levan and levan oligosaccharides can be achieved by glycine supplementation to the growth medium or by toluene­permeabilized whole-cells.

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참고문헌 (34)

  1. Ikura, Y. 1986. Effect of glycine and its derivatives on production and release of beta-galactosidase by Escherichia coli. Agric. Biol. Chem. 50: 2747-2753 
  2. Jang, K. H., J. W. Seo, K. B. Song, C. H. Kim, and S. K. Rhee. 1999. Extracellular secretion of levansucrase from Zymomonas mobilis in Escherichia coli. Bioprocess Eng. 21: 453-458 
  3. Jang, K. H., K. B. Song, C. H. Kim, B. H. Chung, S. A. Kang, U. H. Chun, R. W. Choue, and S. K. Rhee. 2001. Comparison of characteristics of levan produced by different preparations of levansucrase from Zymomonas mobilis. Biotechnol. Lett. 23: 339-344 
  4. Kim, S. H., E. K. Jang, I. H. Kim, K. H. Jang, S. A. Kang, and B. I. Chang. 2003. Effect of glycine supplement on extracellular secretion of levansucrase from seudomonas aurantiaca S-4380 in recombinant Escherichia coli. Korean J. Biotechnol. Bioeng. 18: 312-317 
  5. Leibovici J., S. Kopel, A. Siegel, and O. Gal-Mor. 1986. Effect of tumor inhibitory and stimulatory doses of levan, alone and in combination with cyclophosphamide, on spleen and lymph nodes. Int. J. Immunopharmacol. 8: 391-403 
  6. Ohtsuka, K., S. Hino, T. Fukushima, O. Ozawa, T. Kanematsu, and T. Uchida. 1992. Characterization of levansucrase from Rahnella aquatilis JCM-1683. Biosci. Biotech. Biochem. 56: 1373-1377 
  7. Osborn, M. J., J. E. Gander, E. Parisi, and J. Carson. 1972. Mechanism of assembly of the outer membrane of Salmonella typhimurium; isolation and characterization of cytoplasmic and outer membranes. J. Biol. Chem. 247: 3962-3972 
  8. Rhee, S. K., K. B. Song, C. H. Kim, B. S. Park, E. K. Jang, and K. H. Jang. 2002. Levan, pp 351-377. In S. Alexander, E. Vandamme, and S. De. Baets (eds.), Biopolymers, vol. 5. Wiley-VCH, Weinheim, Germany 
  9. Jang, E. K., K. H. Jang, I. Koh, I. H. Kim, S. H. Kim, S. A. Kang, C. H. Kim, S. D. Ha, and S. K. Rhee. 2002. Molecular characterization of the levansucrase gene from Pseudomonas aurantiaca S-4380 and its expression in Escherchia coli. J. Microbiol. Biotechnol. 12: 603-609 
  10. Tanaka, T., S. Oi, and T. Yamamoto. 1979. Synthesis of levan by levansucrase. J. Biochem. 85: 287-293 
  11. Blight, M. A., C. Chervaux, and I. B. Holland. 1994. Protein secretion pathways in Escherichia coli. Curr. Opin. Biotech. 5: 468-474 
  12. Yanase, H., M. Iwata, R. Nakahigashi, K. Kita, N. Kato, and K. Tonomura. 1992. Purification, crystallization and properties of the extracellular levansucrase from Zymomonas mobilis. Biosci. Biotech. Biochem. 56: 1335-1337 
  13. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 
  14. Loo, J. V., J. Cummings, N. Delzenne, H. Englyst, A. Frank, M. Hopkins, N. Kok, G. Macfalane, D. Newton, M. Quigley, M. Roberfroid, T. van Vliet, and E. van den Heuvel. 1999. Functional food properties of non-digestible oligosaccharides: A consensus report from the ENDO project (DGXII AIRIICT94- 1095). Br. J. Nutr. 81: 121-132 
  15. Sunita, K., B. H. Chung, K. H. Jang, K. B. Song, C. H. Kim, and S. K. Rhee. 2000. Refolding and purification of Zymomonas mobilis levansucrase produced as inclusion bodies in fed-batch culture of recombinant Escherichia coli. Protein Expres. Purif. 18: 388-393 
  16. Kim, H. J., H. E. Park, M. J. Kim, H. G. Lee, J. Y. Yang, and J. H. Cha. 2003. Enzymatic characterization of a recombinant levansucrase from Rahnella aquatilis ATCC 15552. J. Microbiol. Biotechnol. 13: 230-235 
  17. Chalmers, J. J., E. Kim, J. N. Telford, E. Y. Wong, W. C. Tacon, M. L. Shuler, and D. B. Wilson. 1990. Effects of temperature on Escherichia coli overproducing $\beta$-lactamase or human epidermal growth factor. Appl. Environ. Microbiol. 56: 104-111 
  18. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A 
  19. Singh, Y. 2003. Photosynthetic activity, and lipid and hydrocarbon production by alginate immobilized cells of Botrycoccus in relation to growth phase. J. Microbiol. Biotechnol. 13: 687-691 
  20. Hammes, W., K. H. Scheifer, and O. Kandler. 1973. Mode of action of glycine on the biosynthesis of peptidoglycan. J. Bacteriol. 116: 1029-1053 
  21. Bradford, M. M. 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein of protein-dye binding. Anal. Biochem. 72: 248-254 
  22. Patil, N. K., U. Sharanagouda, J. H. Niazi, C. K. Kim, and T. B. Karegoudar. 2003. Degradation of salicylic acid by free and immobilized cells of Pseudomonas sp. strain NGK1. J. Microbiol. Biotechnol. 13: 29-34 
  23. Seo, J. W., K. B. Song, K. H. Jang, C. H. Kim, B. H. Jung, and S. K. Rhee. 2000. Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli. J. Biotechnol. 81: 63-72 
  24. Seo, J. W., K. H. Jang, S. A. Kang, K. B. Song, E. K. Jang, B. S. Park, C. H. Kim, and S. K. Rhee. 2002. Molecular cloning of the growth phase-dependent expression of the lsrA gene, encoding levansucrase of Rahnella aquatilis. J. Bacteriol. 184: 5862-5870 
  25. Chun U. H. and P. L. Rogers. 1988. The simultaneous production of sorbitol from fructose and gluconic acid from glucose using an oxidoreductase of Zymomonas mobilis. App. Microbiol. Biotechnol. 29: 19-24 
  26. John E. Van W. and A. D. Johannis. 1990. How big is the periplasmic space? Trends Biochem. Sci. 15: 136-137 
  27. Pelicic, V., J. M. Reyrat, and B. Gicquel. 1996. Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria. J. Bacteriol. 178: 1197-1199 
  28. Salmond, G. P. C. and P. J. Reeves. 1993. Membrane traffic wardens and protein secretion in Gram-negative bacteria. Trends Biochem. Sci. 18: 7-12 
  29. Calazans, G. M. T., R. C. Lima, F. P. Francisca, and C. E. Lopes. 2000. Molecular weight and antitumor activity of Zymomonas mobilis. Int. J. Biol. Macromol. 27: 245-247 
  30. Goebel, W. and J. Hedgpeth. 1982. Cloning and functional characterization of the plasmid-encoded hemolysin determinant of Escherichia coli. J. Bacteriol. 151: 290-298 
  31. Han Y. W. 1990. Microbial levan. Adv. Appl. Microbiol. 35: 171-194 
  32. Hendry, G. A. F. and R. K. Wallace. 1993. The origin, distribution, and evolutionary significance of fructans. In M. Suzuki and N. J. Chatterton (eds.), Science and Technology of Fructans. CRC Press 
  33. Lyness, E. W. and H. W. Doelle. 1983. Levansucrase from Zymomonas mobilis. Biotechnol. Lett. 5: 345-350 
  34. Song, K. B., H. Belghith, and S. K. Rhee. 1996. Production of levan, a fructose polymer, using an overexpressed recombinant levansucrase. Ann. N. Y. Acad. Sci. 799: 601- 607 

이 논문을 인용한 문헌 (2)

  1. 2006. "" Journal of microbiology and biotechnology, 16(1): 102~108 
  2. 2006. "" Journal of microbiology and biotechnology, 16(5): 683~688 

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