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논문 상세정보

Abstract

The extracellular production of 5-aminolevulinic acid (ALA) by recombinant E. coli BL21 harboring a fusion gene hemA was investigated in a fermenter. For this purpose, the effects of various physiological factors, such as isopropylthio­$\beta$-D-galactopyranoside (IPTG) concentrations and the time of induction, on enzyme activity were studied. Optimum concentrations of glycine and succinic acid were found to be 30 mM and 90 mM, respectively. When the cells were permitted to grow for 2 h prior to the addition of 0.1 mM IPTG, the activity of ALA synthase was higher than when IPTG was initially added. A 36-fold increase in the activity was observed with only 0.1 mM IPTG added. The pH of the medium also influenced the ALA synthase activity with the maximal activity occurring at pH 6.5. In recombinant E. coli extracts, the repeated addition of glycine and D-glucose increased the production of ALA and the inhibited intracellular ALA dehydratase activity, with up to 32 mM ALA being produced in the cultivation.

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이 논문을 인용한 문헌 (2)

  1. 2006. "" Journal of microbiology and biotechnology, 16(9): 1448~1452 
  2. Yang, Dong-Soo ; Park, Moon-Won ; Lim, Soo-Jin ; Kim, Min-Jeong ; Shin, Yu-Ri ; Park, Chan-Soo ; Hyun, Young ; Kang, Dae-Kyung 2009. "Optimizing the Production of 5-Aminolevulinic Acid by Recombinant Escherichia coli Containing the Rhodobacter capsulatus hemA Gene" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 37(2): 153~159 

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