[논문]Lack of O-Polysaccharide Renders Bradyrhizobium japonicum More Resistant to Organic Acid Stress

OH , EUN-TAEX; JU, YOUNG-JUN; KOH, SUNG-CHEOL; KIM, YONG-HWI; KIM, JONG-SUL; SO, JAE-SEONG

Lack of O-Polysaccharide Renders Bradyrhizobium japonicum More Resistant to Organic Acid Stress 원문보기

Journal of microbiology and biotechnology, v.14 no.6, 2004년, pp.1324 - 1326

OH , EUN-TAEX (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University) , JU, YOUNG-JUN (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University) , KOH, SUNG-CHEOL (Division of Civil and Environmental Engineering, Korea Maritime University) , KIM, YONG-HWI (Department of Food Science and Technology, Sejong University) , KIM, JONG-SUL (Division of Life Science, University of Ulsan) , SO, JAE-SEONG (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University)

Abstract ▼ AI-Helper

In previous studies, we isolated an isogenic LPS mutant of Bradyrhizobium japonicum 61A101C, which was completely devoid of O-polysaccharide and had altered cell surface characteristics. Subsequently, the mutated gene was identified, cloned, and used to complement the LPS mutant strain JS314 to restore the phenotype. Since it has been reported that in Escherichia coli LPS O-polysaccharide is involved in resistance to an organic acid such as acetic acid under low pH (Barna et al., Molecular Microbiology 43: 629-640, 2002), we compared the organic acid resistance of the three B. japonicum strains; wild-type 61A101C, the LPS mutant JS314, and the complemented strain to determine whether the role of O-polysaccharide in the resistance to organic acid could be generalized. Growth of all three strains was inhibited by the presence of 3 mM acetic acid under acidic condition (pH 5.5). To our surprise, however, in the presence of 2 mM acetic acid, wild-type and the complemented strains did not grow while the $LPS^-$ mutant showed a significant growth. Therefore, unlike in E. coli, the lack of Opolysaccharide of LPS appears to render B. japonicum more resistant to organic acid.

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참고문헌 (15)

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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