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논문 상세정보

Abstract

In previous studies, we isolated an isogenic LPS mutant of Bradyrhizobium japonicum 61A101C, which was completely devoid of O-polysaccharide and had altered cell surface characteristics. Subsequently, the mutated gene was identified, cloned, and used to complement the LPS mutant strain JS314 to restore the phenotype. Since it has been reported that in Escherichia coli LPS O-polysaccharide is involved in resistance to an organic acid such as acetic acid under low pH (Barna et al., Molecular Microbiology 43: 629-640, 2002), we compared the organic acid resistance of the three B. japonicum strains; wild-type 61A101C, the LPS mutant JS314, and the complemented strain to determine whether the role of O-polysaccharide in the resistance to organic acid could be generalized. Growth of all three strains was inhibited by the presence of 3 mM acetic acid under acidic condition (pH 5.5). To our surprise, however, in the presence of 2 mM acetic acid, wild-type and the complemented strains did not grow while the $LPS^-$ mutant showed a significant growth. Therefore, unlike in E. coli, the lack of O­polysaccharide of LPS appears to render B. japonicum more resistant to organic acid.

참고문헌 (15)

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  2. Oh, E. T., H. S. Yun, T.-R. Heo, S.-C. Koh, K.-H. Oh, and J.-S. So. 2002. Involvement of lipopolysaccharide of Bradyrhizobium japonicum in metal binding. J. Microbiol. Biotechnol. 12: 296-300 
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  6. So, J.-S., W.-S. Kim, and G. Stacey. 2000. Molecular characterization of a gene region involved in lipopolysaccharide biosynthesis in Bradyrhizobium japonicum: Cloning, sequencing and expression of rfaf gene. FEMS Microbiol. Lett. 190: 109-114 
  7. Noh, J., D. H. Kim, E. T. Oh, and J.-S. So. 2002. Characterization of the rfaD gene region of Bradyrhizobium japonicum 61A101C. J. Microbiol. Biotechnol. 12: 826- 828 
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  12. Chung, T.-W., U.-H. Jin, and C.-H. Kim. 2003. Salmonella typhimurium LPS confers its resistance to antibacterial agents of baicalin of Scutellaria baicalensis george and novobiocin: Complementation of the rfaE gene required for ADP-L-glycero-D-manno-heptose biosynthesis of lipopolysaccharide. J. Microbiol. Biotechnol. 13: 564-570 
  13. Foster, J. W. 1999. When protons attack: Microbial strategies of acid adaptation. Curr. Opin. Microbiol. 2: 170-174 
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  15. Park, K.-M. and J.-S. So. 2000. Altered cell surface hydrophobicity of lipopolysaccharide-deficient mutant of Bradyrhizobium japonicum. J. Microbiol. Methods 41: 219- 226 

이 논문을 인용한 문헌 (1)

  1. 2006. "" Journal of microbiology and biotechnology, 16(9): 1416~1421 

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