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Abstract

Oxidants such as hydrogen peroxide are powerful inducers of cell damage, ageing, and apoptosis. Since clusterin, a 75-80 kDa mammalian glycoprotein, is frequently found to be inducible in apoptotic cells and tissues, this study inquired into whether this would be a protective mechanism against further cell death. The aim was to find out whether overexpression of clusterin could protect cells from oxidant­induced stress and apoptosis. To clarify this issue, we generated and analyzed stable cell lines expressing fusion proteins of a rat clusterin with an enhanced green fluorescent protein (EGFP). When treated with varying concentrations of hydrogen peroxides, clusterin transfectants indeed showed increased resistance to apoptosis and exhibited a much higher survival rate than mock-transfected cells. On the other hand, neither intracellular re-distribution nor local concentration of clusterin-EGFP was observed, which leaves the question open about its anti-apoptotic mechanism. In conclusion, the overexpression of clusterin provides a means for protecting cells against oxidative stress and subsequent cell death.

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참고문헌 (22)

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이 논문을 인용한 문헌 (1)

  1. You, Young-Hyun ; Nam, Joo-Hyeung ; Kim, Bieong-Kil ; Kim, Soon-Bok ; Moon, Ik-Jae ; Kim, Jong-Pil ; Seu, Young-Bae 2009. "Biosafety of the New Soft Contact Lens Materials in the Fibroblast L-929 Cell Line" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 37(1): 75~79 

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