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Introduction of Saxicolous Lichens Distributed in Coastal Rocks of U-do Islet in Jeju, Korea

The journal of microbiology, v.42 no.4, 2004년, pp.292 - 298  

Kahng Hyung-Yeel (Department of Environmental Education, Sunchon National University, Korean Lichen Research Institute) ,  Yoon Byoung-Jun (Korean Lichen Research Institute) ,  Kim Sung-Hyun (Department of Environmental Education, Sunchon National University) ,  Shin Duck-Ja (Department of Environmental Education, Sunchon National University) ,  Hur Jae-Seoun (Department of Environmental Education, Sunchon National University, Korean Lichen Research Institute) ,  Kim Hyun-Woo (Department of Environmental Education, Sunchon National University, Korean Lichen Research Institute) ,  Kang Eui-Sung (Korean Lichen Research Institute, Department of Computer Education, Sunchon National University) ,  Oh Kye-Heon (Department of Applied Biology, Sunchon National University) ,  Koh Young Jin (Korean Lichen Research Institute, Department of Life Science, Soonchunhyang University)

Abstract AI-Helper 아이콘AI-Helper

This study reports, for the first time, the ivestigation of the distribution of Korean saxicolous lichens in the coastal rocks of U-do islet, which is known as an unpolluted zone in Jeju. More than thirty lichens were obtained and investigated from the coastal rocks frequently contacted by seawater....

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  • 3, were inspected, and specimens taken that could be removed using a screwdriver, mallet or utensils. All specimens were labelled with the collection date and site, and taken to the laboratory for identification of the level of species (or genus) using a molecular approach based on the intergenic spaces (ITS) of the 5.8S-18S rRNA sequence region along with lichen morphology such as thallus color, thallus classification (crustose, foliose, fruticose or squamulose), apothecia, either irregular or round and cuplike, with or without exciple, and spore characteristics, including color and size. The lichen samples used in this study were chosen due to their different geographic origins.
  • The tree showing the phylogenetic relationship between the lichens found in U-do Islet and their identities. Approx 400-450 bp 5.8S rRNA sequences, containing the ITS region, of the lichens living on the coastal rocks in the U-do islet, were analyzed and used to construct the dendrogram. The rDNA ITS sequences from genera Graphis and Ochrolechia were not used to make the tree due to lack of the rDNA ITS sequences in the analysed data.
  • 8S- ITS2). Information on the length heterogeneity of the ITS1-5.8S-ITS2 region was examined by searching the GenBank database to assess the extent of variability within the main fungal taxonomic groups.
  • 1 with sterile water. PCR was performed in a PCR machine (Perkin Elmer, USA) with the following thermo-cycling program: 5 min denaturation at 95℃, followed by 33 cycles of 1 min denaturation at 95℃, 1 min annealing at 55℃, 1 min extension at 72℃, and a final extension step of 10 min at 72℃. Ten microliters of PCR products were visualized by electrophoresis in 2% (w/v) agarose gels, with ethidium bromide (0.
  • , 2004), but very limited knowledge or information on saxicolous lichens, especially distributed in coastal rocks, is available. Therefore, this study aimed at creating an inventory of the more common saxicolous lichens in coastal rocks of U-do, which is located in 33°3O'N, 126°56'E (Fig. 2). This study reports for the first time on the saxicolous lichens distributed in the coastal rocks of U-do frequently contacted by seawater using both genetic and morphological analyses.
  • 2). This study reports for the first time on the saxicolous lichens distributed in the coastal rocks of U-do frequently contacted by seawater using both genetic and morphological analyses.
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참고문헌 (22)

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  18. Normand, P., C. Ponsonnet, X. Nesme, M. Neyra, and P. Simonet. 1996. ITS analysis of prokaryotes, p. 1-12. In D.L. Akkermans, J.D. van Elsas, and E.I. de Bruijn (ed.), Molecular microbial ecology manual. Kluwer Academic Publishers, Amsterdam, The Netherlands 

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