$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Axenic Culture of Gyrodinium impudicum Strain KG03, a Marine Red-tide Microalga that Produces Exopolysaccharide

The journal of microbiology v.42 no.4 , 2004년, pp.305 - 314  
Abstract

An exopolysaccharide-producing microalgal dinoflagellate was isolated from a red-tide bloom and des­ignated strain KG03. A bacteria-free culture of strain KG03 was achieved using a modified wash with phototaxis and antibiotic treatment. Combined treatment with neomycin and cephalosporin was the most effective for eliminating the bacteria associated with the microalgae. Strain KG03 was identified as Gyrodinium impudicum by analyzing the ITS regions of the 5.8S rDNA, 18S rDNA, morphological phenotype and fatty acid composition. The exopolysaccharide production and cell growth in a 300-ml photobioreactor were increased 2.7- and 2.4-fold, respectively, compared with that in a flask culture at the first isolation step.

참고문헌 (22)

  1. Hanson, G., N. Daugbjerg, and P. Henriken. 2000. Comparative study of Gymnodinium mikimoto and Gymnodinium aureolum, comb. nov. (=Gymnodinium aureolum) based on morphology, pigment composition, and molecular data. J. Phycol. 36, 394-410 
  2. Park, J.G. and Y.S. Park. 1999. Comparison of morphological characteristics and the 24S rRNA sequences of Cochlodinium polykrikoides and Gyrodinium impudicum. J. Kor. Soc. Oce. 4, 363-370 
  3. Sako, Y., C.H. Kim, and Y. Ishida. 1992. Mendelian inheritance of paralytic shellfish poisoning in the marine dinoflagellate Alexandrium catenella. Biosci. Biotechnol. Biochem. 56, 692-694 
  4. Imai, I. and M. Yamaguchi. 1994. A simple technique for establishing axenic cultures of phytoflagellates. Bull. Jpn. Soc. Microb. Ecol. 9, 15-17 
  5. Adachi, M., Y. Sako, and Y. Ishida. 1996. Analysis of Alexandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions. J. Phycol. 32, 424-432 
  6. Droop, M.R. 1967. A procedure for routine purification of algal cultures with antibiotics. Br. Phycol. Bull. 3, 295-297 
  7. Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers, and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 38, 350-356 
  8. Robert R. and L. Guillard. 1973 In. Stein J. R. (eds.) Handbook of Phycological Methods, p. 289-311. Cambridge University Press, Cambridge, UK 
  9. Thompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680 
  10. Cho, E.S., G.Y. Kim, B.D. Choi, L.L. Rhodes, T.J. Kim, G.H. Kim, and J.D. Lee. 2001. A comparative study of harmful dinoflagellates Cochlodinium polykrikoides and Gyrodinium impudicum using transmission electron microscopy, fatty acid composition, carotenoid content, DNA quantification and gene sequences. Botanica Marina 44, 57-66 
  11. Lewin, R.A. 1959. The isolation of algae. Res. Algol. 3, 181-19 
  12. Pringsheim, E.G. 1951. Handbook of Biological Methods, AbtXI (II), p. 377-406. AlgenKultur 
  13. Vincenzini, M., R. De Philippis, C. Sili, and D. Materassi. (1990). A novel exopolysaccharide from a filamentous cyanobacterium: production, chemical characterization and rheological properties, p. 295-310. In E. A. Dawes (ed.), Novel biodegradable microbial polymers, Kluwer Academic Publishers, Dordrecht. 
  14. Guillard, R.R.L. 1975. Culture of phytoplankton for feeding marine invertebrates, p. 29-60. In Smith, W.L. and M.H. Chanley (eds.), Culture of Marine Invertebrate Animals. Plenum, New York 
  15. Singh, H.T., Y. Oshima, and T. Yasumoto. 1982. Growth and toxicity of Protogonyaulax tamarensis in axenic culture. Bull. Jpn. Soc. Sci. Fish 48, 1341-1343 
  16. Doucette, G.J., and C.L. Powell. 1998. Algal-bacterial interactions: Can they determine the PSP-related toxicity of dinoflagellates? p. 406-409. In Reguera, B. (ed.), Harmful Algae. Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO 
  17. Fogg, G., and B. Thake. 1987. Algal Cultures and Phytoplankton Ecology, p. 19-20. The University of Wisconsin Press, Madison 
  18. Chun, J. 1995. Computer-assisted classification and identification of actinomycetes. Ph.D. Thesis. Univ. Newcastle, Newcastleupon-Tyne, UK 
  19. Linton, J.D. 1990. The relationship between metabolite production and the growth efficiency of the producing organisms. FEMS Microbiol Rev. 75, 1-18 
  20. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425 
  21. Sutherland, I.W. 1990. Biotechnology of microbial exopolysaccharides. Cambridge studies in Biotechnology, vol. 9. Cambridge University Press, Cambridge, UK 
  22. Yim, J.H., S.J. Kim, S.H. Ahn, J.H. Lee, K.T. Rhie, and H.G. Lee. 2004. Antiviral effects of sulfated exopolysaccharide from the marine microalga Gyrodinium impudicum strain KG03. Mar. Biotechnol. 6, 17-25 

이 논문을 인용한 문헌 (1)

  1. Youn, Joo-Yeon ; Hur, Sung-Bum 2007. "Antibiotics and Their Optimum Concentration for Axenic Culture of Marine Microalgae" Algae, 22(3): 229~234 

DOI 인용 스타일