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선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- Seeds were taken out from the aging box daily and stored in a freezer at -12 °C after dry ing at room temperature A germination test was con ducted in moist paper towels according to the Association of Official Seed Analyst (AOSA, 1990). Aged seeds of which germination rate was about half of the normal seeds were made by aging dent and sweet coms for 10 days and super sweet com for 3 days according to the results of the preliminary experiment.
- In this study, we investigated the optimum WP for the seed priming of normal and aged seeds of three com types and the effects of priming on the emergence, a-amylase activity, leakage of total sugars and electrolytes m soaking solution, and changes m DNA and sohible protein content dunng the seed priming.
대상 데이터
- Seeds were ground in a cyclone mill (1093 Cyclotec Sam ple Mill, Foss Tecator, Sweden) to pass a 100-mesh screen. For both DNA and protein analyses, 0.
데이터처리
- 'Means within a column for a given com type followed by the same letter are not significantly different at the 5% level by Duncan's New Multiple Range Test (DNMRT).
이론/모형
- The a-amylase activity of the seeds was measured by the method designed by Reiss (1994). Three seeds were soaked m distilled water at 25 °C for 7 days, dipped in liquid nitro gen, and ground in a mortar with a pestle.
- 5-mL e-tube, 2 |1L ofRNase (M610A, Promega, USA) was added, reacted at 37 °C for 30 minutes, and washed with 99 and 70% ethanol in order. The residue was dissolved in 200 uL TE buffer (10 mM Tri-HCl, 1 mM EDTA in 1,000 mL water) and the absorbance ofsample was measured at 260 nm for DNA and at 280 nm for protein with a spectrophotometer (UVIKON Spectrophotometer, Konton, Italy) according to the method of Nam & Ahn (1999).
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