[논문]Effects of Sonication and Vacuum Infiltration on Agrobacterium-Mediated Transformation in Immature Embryos of Korean Wheat Genotypes

Moon Jung-Hun; Kang Moon-Suk; Heo Hwa-Young; Kwon Young-Up; Lee Sang-Kyu; Lee Kyung-Hee; Lee Byung-Moo

Effects of Sonication and Vacuum Infiltration on Agrobacterium-Mediated Transformation in Immature Embryos of Korean Wheat Genotypes 원문보기

Korean journal of crop science = 韓國作物學會誌, v.49 no.5, 2004년, pp.415 - 418

Moon Jung-Hun (Dept. of Plant Resources, Dongguk Univ.) , Kang Moon-Suk (National Institute of Crop Science, RDA) , Heo Hwa-Young (National Institute of Crop Science, RDA) , Kwon Young-Up (National Institute of Crop Science, RDA) , Lee Sang-Kyu (Dept. of Plant Resources, Dongguk Univ.) , Lee Kyung-Hee (Dept. of Plant Resources, Dongguk Univ.) , Lee Byung-Moo (Dept. of Plant Resources, Dongguk Univ.)

Abstract ▼ AI-Helper

The effects of sonication and vacuum infiltration on transformation efficiency was investigated by using immature embryos of Korean wheat as explants. Two Agrobacterium tumefaciens strains, KYRT1 and EHA105, carrying pCAMBIA 1305.1 were used. Transformation efficiency was demonstrated by the detection of $\beta-glucu-ronidase$ (GUS) activity. GUS expression showed clear difference among Korean wheat cultivars. Geurumil showed higher GUS expression efficiency $79.1\%$ compared with other cultivars. The effects of the duration of vacuum infiltration and sonication treatment showed a tendency high GUS expression efficiency by their combination. In comparison with other Agrobacterium strains, KYRT1 showed high efficiency in most Korean cultivars.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

This experiment was thus designed to investigate the effects of the duration of vacuum and sonication treatments on GUS expression in Korean wheat cultivars. There treatments significantly increased the number of explants producing GUS expression (Table 1).

제안 방법

The purpose of this paper was to determine the effects of sonication and vacuum infiltration on transformation and to develop an efficient method of Agrobacterium-medi2d.ed transformation by using immature embryos of Korean wheat cultivars.

대상 데이터

Alchanmil, Geurumil, Gobunmil, Keumkangmil, Tapdongmil, Unmil) were used as the target matenals for transformation efficiencies. The cultivars were planted in the field during the normal wheat growing season (2002 - 2003) at the National Institute of Crop Science, Suwon, Korea. Immature embiyos measuring L0 ~ 2.

성능/효과

In conclusion, optimal T-DNA delivery as measured by GUS activity was obtained in KYRT1 treatment and combination of sonication and vacuum infiltration treatments. Especially the frequency of GUS expression showed clearly difference among the wheat cultivars.

참고문헌 (20)

Amoah, B. K., H Wu, C. Sparks, and H. D. Jones 2001 Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue. Journal of Experimental Botany 52. 1135-1142

상세보기
Chang, S. S., S. K Park, B. C. Kim, B J. Kang, and D. U. Kim. 1994 Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta The Plant J 5 : 551-558

상세보기
Cheng, M, J. E. Fry, S. Pang, H. Zhou, C, M. Hironaka, D R. Duncan, T W. Conner, Y. Wan 1997 Genetic transformation of wheat mediated by Agrobacterium tumefaciens Plant Physiol. 115 : 971-980

상세보기
Clough, S. J. and A. F. Bent. 1998 Floral dip. a Simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant J. 16 : 735-743

상세보기
Hood, E. E., S. B. Gelvin, L. S. Melchers, and A. Hoekema. 1993. New Agrobacterium helper plasmids for gene transfer to plants Transgenic Res. 2 208-218

상세보기
Jefferson, R A 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. BioI Rep. 5 : 387-405

상세보기
Joersbo, M. and J. Brunstedt 1990. Direct gene transfer to plant protoplasts by mild sonication Plant Cell Rep. 9 : 207-210

상세보기
Joersbo, M. and J. Brunstedt. 1992 Sonication. A new method for gene transfer to plants. Physiology Plantarum 85:230-234

상세보기
Lee, B. M. 2001. Effect of sonication and vir genes on transient gene expression in Agrobacterium-mediated transformation. Korean J of LIfe Sci 11 : 316-320
Mysore, K. S., C T R. Kumar, and S. B. Gelvin. 2000. Arabidopsis ecotypes and mutants that are recalcitrant to Agrobacterium root transformation are susceptible to germ-line transformation The Plant J. 21 : 9-16

상세보기
Tingay, S., D. McElroy, R Kalla, S. Fieg, M. B. Wang, S. Thornton, and R. Brettell. 1997. Agrobacterium tumefaciens-mediated barley transformation The Plant J 11 . 1369-1376

상세보기
Trick, H N and J. J Finer. 1997. SAAT: sonication-assisted Agrobacterium- mediated transformation Transgenic Res. 6 . 329-336

상세보기
Trick, H N and J. J. Finer. 1998 Sonication-assited Agrobacterium- mediated transformation of soybean 'Glycine max (L.) Merrill' embryonic suspension culture tissue Plant Cell Rep. 17 : 482-488

상세보기
Trick, H N. and J J Finer. 1999 Induction of Somatic embryogenesis and genetic transformation of Ohio buckeye (Aesculus glabra Willd.). In Vitro Cell Dev. BioI. 35 : 57-60

상세보기
Torisky, R. S., L. Kovacs, S. Avdiushko, J. D. Newman, A. G. Hunt, and G. B. Collins 1997. Development of a binary vector system for plant transformation based on the supervirulent Agrobacterium tumefaciens strain Chry5 Plant Cell Rep. 17. 102-108

상세보기
Wang, M , D. C. Abbott, N. M Upadhyaya, J. V. Jacobsen, and P M. Waterhouse 2001. Agrobacterium tumefaciens-mediated transformation of an elite Australian barley cultivar with virus resistance and reporter genes Aust. J. Plant Physiol. 28 . 149-156

상세보기
Weir, B., X. Gu, M. B. Wang, N Upadhyaya, A. R. Elliott, and R. I S. Brettell. 2001 Agrobacterium tumefaciens-mediated transformation of wheat using suspension cells as a model system and green fluorescent protein as a visual marker Aust J. Plant Physiol, 28 807-818

상세보기
Wu, H., A C. McCormac, M. C. Elliott, and D. F. Chen 1998 Agrobacterium-mediated stable transformation of suspension cultures of barley (Hordeum vulgare L.). Plant Cell Tissue Organ Cult. 54 : 161-167
Wu, H., C. Sparks, B. Amoah, H. D Jones. 2003 Factors influencing successful Agrobacterium-mediated genetic transformation of wheat. Plant Cell Rep 21 : 695-668
Ye, G N , D Stone, S. Z Pang, W. Creely, K. Gonzalez, and M. Hinchee. 1999. Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation The Plant J 19 . 249-257

상세보기

저자의 다른 논문 :

원문 보기

ScienceON 원문보기

원문 URL 링크

한국작물학회 : 저널
코리아스칼라 : 저널

*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.

ScienceON TREND 유전자 발현 비교

연관된 기능

코센리포트

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관

저장형식

Text(ASCII format)
Excel format
RefWorks Direct Export
RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

연합인증