$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

The effects of sonication and vacuum infiltration on transformation efficiency was investigated by using immature embryos of Korean wheat as explants. Two Agrobacterium tumefaciens strains, KYRT1 and EHA105, carrying pCAMBIA 1305.1 were used. Transformation efficiency was demonstrated by the detection of $\beta-glucu-ronidase$ (GUS) activity. GUS expression showed clear difference among Korean wheat cultivars. Geurumil showed higher GUS expression efficiency $79.1\%$ compared with other cultivars. The effects of the duration of vacuum infiltration and sonication treatment showed a tendency high GUS expression efficiency by their combination. In comparison with other Agrobacterium strains, KYRT1 showed high efficiency in most Korean cultivars.

저자의 다른 논문

참고문헌 (20)

  1. Mysore, K. S., C T R. Kumar, and S. B. Gelvin. 2000. Arabidopsis ecotypes and mutants that are recalcitrant to Agrobacterium root transformation are susceptible to germ-line transformation The Plant J. 21 : 9-16 
  2. Trick, H N and J. J. Finer. 1998 Sonication-assited Agrobacterium- mediated transformation of soybean 'Glycine max (L.) Merrill' embryonic suspension culture tissue Plant Cell Rep. 17 : 482-488 
  3. Weir, B., X. Gu, M. B. Wang, N Upadhyaya, A. R. Elliott, and R. I S. Brettell. 2001 Agrobacterium tumefaciens-mediated transformation of wheat using suspension cells as a model system and green fluorescent protein as a visual marker Aust J. Plant Physiol, 28 807-818 
  4. Ye, G N , D Stone, S. Z Pang, W. Creely, K. Gonzalez, and M. Hinchee. 1999. Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation The Plant J 19 . 249-257 
  5. Lee, B. M. 2001. Effect of sonication and vir genes on transient gene expression in Agrobacterium-mediated transformation. Korean J of LIfe Sci 11 : 316-320 
  6. Torisky, R. S., L. Kovacs, S. Avdiushko, J. D. Newman, A. G. Hunt, and G. B. Collins 1997. Development of a binary vector system for plant transformation based on the supervirulent Agrobacterium tumefaciens strain Chry5 Plant Cell Rep. 17. 102-108 
  7. Joersbo, M. and J. Brunstedt. 1992 Sonication. A new method for gene transfer to plants. Physiology Plantarum 85:230-234 
  8. Tingay, S., D. McElroy, R Kalla, S. Fieg, M. B. Wang, S. Thornton, and R. Brettell. 1997. Agrobacterium tumefaciens-mediated barley transformation The Plant J 11 . 1369-1376 
  9. Clough, S. J. and A. F. Bent. 1998 Floral dip. a Simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant J. 16 : 735-743 
  10. Trick, H N. and J J Finer. 1999 Induction of Somatic embryogenesis and genetic transformation of Ohio buckeye (Aesculus glabra Willd.). In Vitro Cell Dev. BioI. 35 : 57-60 
  11. Cheng, M, J. E. Fry, S. Pang, H. Zhou, C, M. Hironaka, D R. Duncan, T W. Conner, Y. Wan 1997 Genetic transformation of wheat mediated by Agrobacterium tumefaciens Plant Physiol. 115 : 971-980 
  12. Wang, M , D. C. Abbott, N. M Upadhyaya, J. V. Jacobsen, and P M. Waterhouse 2001. Agrobacterium tumefaciens-mediated transformation of an elite Australian barley cultivar with virus resistance and reporter genes Aust. J. Plant Physiol. 28 . 149-156 
  13. Trick, H N and J. J Finer. 1997. SAAT: sonication-assisted Agrobacterium- mediated transformation Transgenic Res. 6 . 329-336 
  14. Amoah, B. K., H Wu, C. Sparks, and H. D. Jones 2001 Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue. Journal of Experimental Botany 52. 1135-1142 
  15. Hood, E. E., S. B. Gelvin, L. S. Melchers, and A. Hoekema. 1993. New Agrobacterium helper plasmids for gene transfer to plants Transgenic Res. 2 208-218 
  16. Jefferson, R A 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. BioI Rep. 5 : 387-405 
  17. Wu, H., C. Sparks, B. Amoah, H. D Jones. 2003 Factors influencing successful Agrobacterium-mediated genetic transformation of wheat. Plant Cell Rep 21 : 695-668 
  18. Joersbo, M. and J. Brunstedt 1990. Direct gene transfer to plant protoplasts by mild sonication Plant Cell Rep. 9 : 207-210 
  19. Wu, H., A C. McCormac, M. C. Elliott, and D. F. Chen 1998 Agrobacterium-mediated stable transformation of suspension cultures of barley (Hordeum vulgare L.). Plant Cell Tissue Organ Cult. 54 : 161-167 
  20. Chang, S. S., S. K Park, B. C. Kim, B J. Kang, and D. U. Kim. 1994 Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta The Plant J 5 : 551-558 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

이 논문과 연관된 기능

DOI 인용 스타일