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Soybean is a major source of protein meal in the world. Kunitz trypsin inhibitor (KTI) protein is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to identify RAPD markers linked to KTI protein allele using bulked segregant analysis. Cultivar Jinpumkong2 (TiTi) was crossed with C242 (titi, absence of KTI protein) and F. seeds were planted. The $F_1$. plants were grown in the greenhouse to produce $F_2$ seeds. Each $F_2$ seed from $F_1$. plants was analysed electrophoretically to determine the presence of the KTI protein band. The present and absent bulks contained twenty individuals each, which were selected on the basis of the KTI protein electrophoresis, respectively. Total 94 $F_2$ individuals were constructed and 1,000 Operon random primers were used to identify RAPD primers linked to the Ti locus. The presence of KTI protein is dominant to the lack of a KTI protein and Kunitz trypsin inhibit protein band is controlled by a single locus. Four RAPD primers (OPAC12, OPAR15, OPO12, and OPC08) were linked to the Ti locus. RAPD primer OPO12 was linked to Ti locus, controlling kunitz trypsin inhibitor protein at a distance of 16.0 cM. This results may assist in study of developing fine map including Ti locus in soybean.

참고문헌 (16)

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  8. Rackis, J. J., H. A. Sasame, R. R. Mann, R L Anderson, and A K. Smith. 1962 Soybean trypsin inhibitors: Isolation, purification and physical properties Arch Biochem. Biophys. 98 : 471-478 
  9. Michelmore, R W., J Paran, and R V Kesseli. 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregation populations. Proc. Natl. Acad. Sci. 88 : 9828-9832 
  10. Hymowitz, T. and H. H Hadley. 1972 Inheritance of a trypsin inhibitor variant in seed protein of soybeans. Crop Sci. 12 . 197-198 
  11. Singh, L., C. M. Wilson, and H H Hadley 1969. Genetic differences in soybean trypsin inhibitors separated by disc electrophoresis Crop Sci 9 : 489-491 
  12. Lander, E., P Green, J Abrahamson, A. Barlow, M J. Daly, S E. Lincoln, and L Newburg 1987 MAPMAKER. An interactive computer package for constructing primary genetic linkage maps of experiment and natural populations. Genomics 1 . 174-181 
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  14. Saghai Maroof, M. A., K M Soliman, R. A. Jorgensen, and R. W. Allard 1984. Ribosomal DNA spacer-length polyrmorphisms in barley. Mendelian inheritance, chromosomal Iocation and population dynamics Proc Natl. Acad Sci. 81 . 8014-8018 
  15. Birk, Y. 1961.Purification and some properties of a highly active inhibitor of trypsin and chymotrypsin from soybeans Biochem. Biophys. Acta 54 : 378-381 
  16. Cregan,P. B., T. Jarvik, A L. Bush, R. C. Shoemaker, K. G Lark, A L Kahler, N. Kaya, T. T. VanToai, D. G Lohnes, Jongil Chung, and J E. Specht. 1999 An integrated genetic linkage map of the soybean genome. Crop Sci. 39 . 1464-1490 

이 논문을 인용한 문헌 (1)

  1. 2007. "" Korean journal of crop science = 韓國作物學會誌, 52(1): 51~54 


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