[논문]Identification of Molecular Markers Linked to Ti Locus in Soybean

Kim Myung Sik; Park Min Jung; Hwang Jung Gyu; Jo Soo Ho; Ko Mi Suk; Chung Jong Il

Identification of Molecular Markers Linked to Ti Locus in Soybean 원문보기

Korean journal of crop science = 韓國作物學會誌, v.49 no.5, 2004년, pp.419 - 422

Kim Myung Sik (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) , Park Min Jung (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) , Hwang Jung Gyu (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) , Jo Soo Ho (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) , Ko Mi Suk (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) , Chung Jong Il (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University)

Abstract ▼ AI-Helper

Soybean is a major source of protein meal in the world. Kunitz trypsin inhibitor (KTI) protein is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to identify RAPD markers linked to KTI protein allele using bulked segregant analysis. Cultivar Jinpumkong2 (TiTi) was crossed with C242 (titi, absence of KTI protein) and F. seeds were planted. The $F_1$. plants were grown in the greenhouse to produce $F_2$ seeds. Each $F_2$ seed from $F_1$. plants was analysed electrophoretically to determine the presence of the KTI protein band. The present and absent bulks contained twenty individuals each, which were selected on the basis of the KTI protein electrophoresis, respectively. Total 94 $F_2$ individuals were constructed and 1,000 Operon random primers were used to identify RAPD primers linked to the Ti locus. The presence of KTI protein is dominant to the lack of a KTI protein and Kunitz trypsin inhibit protein band is controlled by a single locus. Four RAPD primers (OPAC12, OPAR15, OPO12, and OPC08) were linked to the Ti locus. RAPD primer OPO12 was linked to Ti locus, controlling kunitz trypsin inhibitor protein at a distance of 16.0 cM. This results may assist in study of developing fine map including Ti locus in soybean.

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문제 정의

Molecular markers linked to SKTI allele were not published. The objective of this research was to use bulked segregant analysis to identify RAPD markers linked to SKTI allele.

대상 데이터

Of the 1,000 RAPD primers tested on the two parents of cultivar Jinpumkong2 (7777) and C₂₄2 (titi), 124 polymorphic primers were identified. Approximately 12 % primers produced polymorphic DNA fragment differences between the parents.
(1991) was used. The present and absent bulks contained twenty individuals each, which were selected on the basis of the Kunitz trypsin inhibitor protein electrophoresis, respectively.

이론/모형

2 % TBE agarose gels and were stained with EtBr to reveal DNA segments of varying sizes Gels were photographed under transmitted UV light. For the construction of present and absent Kumtz trypsin inhibitor protein band bulks, the methodology of bulked segregant analysis (BSA), described by Mi사lelmore et al. (1991) was used. The present and absent bulks contained twenty individuals each, which were selected on the basis of the Kunitz trypsin inhibitor protein electrophoresis, respectively.

참고문헌 (16)

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Orf, J H. and T Hymowitz 1979. Inheritance of the absence of the Kunitz trypsin Inhibitor in seed protein of soybeans. Crop Sci. 19 : 107-109

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Rackis, J. J., H. A. Sasame, R. R. Mann, R L Anderson, and A K. Smith. 1962 Soybean trypsin inhibitors: Isolation, purification and physical properties Arch Biochem. Biophys. 98 : 471-478

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Singh, L., C. M. Wilson, and H H Hadley 1969. Genetic differences in soybean trypsin inhibitors separated by disc electrophoresis Crop Sci 9 : 489-491

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Saghai Maroof, M. A., K M Soliman, R. A. Jorgensen, and R. W. Allard 1984. Ribosomal DNA spacer-length polyrmorphisms in barley. Mendelian inheritance, chromosomal Iocation and population dynamics Proc Natl. Acad Sci. 81 . 8014-8018

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Westfall, R. L. and S. M. Hauge 1948 The nutritive quality and the trypsin inhibitor content of soybean flour heated at various temperatures J Nutr 35 379-389

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Williams J. G. K., A. R Kubelik, K J Livak, J. A Rafalski, and S V. Tingey 1990 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers Nucleic Acid Rec 18 : 6531-6535

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