$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

A single recessive gene, rxp, controls the bacterial leaf pustule (BLP) resistance in soybean and in our previous article, it has been mapped on linkage group (LG) D2 of molecular genetic map of soybean. A total of 130 recombinant inbred lines (RILs) from a cross between BLP-resistant SS2-2 and BLP-susceptible Jangyeobkong were used to identify molecular markers linked to rxp. Fifteen simple sequence repeat (SSR) markers on LG D2 were screened to construct a genetic map of rxp locus. Only four SSR markers, Satt135, Satt372, Satt448, and Satt486, showed parental polymorphisms. Using these markers, genetic scaffold map was constructed covering 26.2cM. Based on the single analysis of variance, Satt372 among these four SSR markers was the most significantly associated with the resistance to BLP. To develop new amplified fragment length polymorphism (AFLP) marker linked to the resistance gene, bulked segregant analysis (BSA) was employed. Resistance and susceptible bulks were made by pooling equal amount of genomic DNAs from ten of each in the segregating population. A total of 192 primer combinations were used to identify specific bands to the resistance, selecting three putative AFLP markers. These AFLP markers produced the fragment present in SS2-2 and the resistant bulk, and not in Jangyeobkong and the susceptible bulk. Linkage analysis revealed that McctEact97 $(P=0.0004,\;R^2=14.67\%)$ was more significant than Satt372, previously reported as the most closely linked marker.

참고문헌 (18)

  1. Bernard, R. L. and M G Weiss 1973 Qualitative genetics. In B E. Caldwell. ed p 117-154. Soybean improvement, production and uses. ASA and Madison, WI 
  2. Keim, P., T. C. Olson, and R. C. Shoemaker. 1988. A rapid protocol forisolating soybean DNA. Soybean Genet Newsl 15 150-154 
  3. Lander E S , P. Green, J Abrahamson, A. Barlow, M. J. Daly, S E Lincoln, and L. Newburg. 1987. Mapmaker. an interactive computer package for constructing primary genetic maps of experimental and natural populations Genomics 1 : 174-181 
  4. Palmer, R. G., S. M. Lim, and B. R. Hedges 1992 Testing for linkage between the Rxp locus and nine isozyme loci in soybean Crop Sci 32 681-683 
  5. Tanksley, S. D , N. D Young, A. H. Paterson, and M W Bionierbale 1989 RFLP mapping in plant breeding: new tool for an old science Bio/technology 7 257-264 
  6. Tsunematsu, H , A Yoshimura, M Yano, T Sasaki, and N. Iwata. 1996 RFLP framework map using recombinant lines in rice. Breeding Sci 46 279-284 
  7. Van, K., B -K Ha, E -Y Hwang, M Y. Kim, N.-C. Pack, S. Heu, and S.-H. Lee. 2004 SSR Mapping of genes conditioning soybean resistance to six isolate of Xanthomonas axonopodis pv glycines. Korean J. Genet. 26 . 47-54 
  8. Cregan, P B , T Jarvik, A L. Bush, R. C Shoemaker, K. G Lark, A L. Kahler, T. T. Van Toai, D. G. Lohnes, J Chung, and J. E. Specht 1999 An integrated linkage map of the soybean genome. Crop Sci 39 . 1464-1490 
  9. Narvel, J. M., L. R. Jakkula, D. V Philips, T Wang, S-H Lee, and H. R. Boerma. 2000. Molecular mapping and pedigree analysis of the Rxp locus controlling bacterial pustule resistance in soybean. J. Hered. 92 . 267-270 
  10. Hartwig, E E and S. G Lehman 1951. Inheritance of resistance to the bacterial pustule disease in soybean Agron. J 43 22-23 
  11. Michelmore, R W, I Paran, and R. V Kessel. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad Sci 88 9828-9832 
  12. Ziegle, J. S , Y Su, K. P. Corcoran, L. Nie, P E Mayrand, L B. Hoff, L. J. McBride, M. N. Kronick, and S. R. Kiehl 1992 Application of automated DNA sizing technology for genotyping microsatellite loci Genomics 14 . 1026-1031 
  13. Diwan, N and P B Cregan 1997 Automated sizing of fluorescent-labeled simple sequence repeat (SSR) marker to assay genetic variation soybean. Theor. Appl. Genet. 95 . 723-733 
  14. Feaster, C. V. 1951. Bacterial pustule disease on soybean. article inoculation, varietal response, and inheritance of resistance Missouri Agricultural Experiment Station Bulletin p 487 
  15. Vos, P., R. Hogers, M Bleeker, and M. Zabeau. 1995. AFLP. A new technique for DNA fingerprinting. Nucl. Acids Res 23 4407-4414 
  16. Wang S., C J. Basten, and Z.-B. Zeng 2002. Windows QTL Cartographer 2.0. Department of Statistics, North Carolina State University, Raleigh, NC. 
  17. Weber, C. R., J. M Dunleavy, and W. R. Fehr 1996 Effect of bacterial pustule on closely related soybean lines Agron. J 58 : 544-545 
  18. Kosambi, D. D. 1944. The estimation of map distances from recombination values. Ann. Eugen. 12 : 172-175 

이 논문을 인용한 문헌 (1)

  1. 2011. "" Journal of crop science and biotechnology, 14(2): 119~123 

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일