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Transformation of Medicago truncatula with rip1-GUS Gene

Korean journal of crop science = 韓國作物學會誌, v.49 no.5, 2004년, pp.434 - 439  

Nam Young-Woo (Department of Life Science, Sogang University) ,  Song Dae-Hae (Department of Life Science, Sogang University)

Abstract AI-Helper 아이콘AI-Helper

Medicago truncatula is a model plant for molecular genetic studies of legumes and plant-microbe interactions. To accelerate finding of genes that play roles in the early stages of nodulation and stress responses, a trans-genic plant was developed that contains a promoter­reporter fusion. The promote...

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제안 방법

  • To enrich the pool of mutagenizable legumes and increase the possibility for screening early nodulation genes, reporter gene fusions were constructed and introduced into M. trun- catula in this study. The transgenic plants prepared by infiltration of Agrobacterium provide resources for isolating genes involved in legume-speciec processes.

대상 데이터

  • M. truncatula Gaertn Jemalong (A17) was used. Plants were grown in 7 cm diameter pots, one plant per pot.
  • The pSLJ525YN vector served as a vehicle to carry the ripl fusions. Restriction fragments of pRG-FR and pRL-rN, which contained GUS and luciferase genes, respectively (Fig. 1C), were inserted into the vector. The resulting fusion constructs, pSLJ525YN-GUS and pSLJ525YN-luc, were subsequently used for plant transformation.
  • The seeds were then washed 3 times in sterile distilled water and spread on filter paper in petri plates (10 x 100 mm2), approximately 20 seeds per plate. The plates were wrapped with parafilm and aluminum foil. The seeds were vernalized by incubation at 4℃ for 12-18 days.
  • Activities of luciferase (/wc; pRL-rN) and p- glucuronidase (GUS; pRG-FR) were measured quantitatively and their ratios were plotted as a function of test plasmid concentration. Three to five replicates were used per measurement. Bars indicate standard errors
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참고문헌 (24)

  1. Barker, D. G., S. Bianchi, F Blondon, Y. Dattee, G. Duc, S. Essad, P. Flament, P. Gallusci, G Genier, P. Guy, X. Muel, J Tourneur, J. Denarie, and T Huguet 1990 Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobiumlegume symbiosis. Plant Mol. Biol Rep 8 : 40-49 

  2. Bell, C J., R A Dixon, A D. Farmer, R. Flores, J. Inman, R. A Gonzales, M. J. Harrison, N. L. Paiva, A D Scott, J. W. Weller, and G D May. 2001. The Medicago genome initiative: a model legume database. Nucleic Acids Res. 29 : 114-117 

  3. Blondon, F, D. Marie, S. Brown, and A. Kondorosi 1994. Genome size and base composition in Medicago sativa and Medicago truncatula species. Genome 37 264-270 

  4. Catoira, R , C Galera, F de Billy, R. V. Penmetsa, E P Journet, F Maillet, C. Rosenberg, D. Cook, C. Gough, and J. Denarie. 2000. Four genes of Medicago truncatula controlling components of a nod factor transduction pathway. Plant Cell 12 1647-1666 

  5. Chen, D -H. and P C Ronald. 1999. A rapid DNA minipreparation method suitable for AFLP and other PCR applications Plant Mol Biol Rep 17 . 53-57 

  6. Cook, D. R. 1999. Medicago truncatula a model in the making! Curr Opin. Plant Biol. 2 301-304 

  7. Cook, D., D. Dreyer, D. Bonnet, M. Howell, E Nony, and K. VandenBosch 1995 Transient induction of a peroxidase gene in Medicago truncatula precedes infection by Rhizobium meliloti Plant Cell 7 43-55 

  8. Gallagher, S R 1992 Quantitation of GUS activity by fluorometry In GUS Protocols, Gallagher SR ed, Academic Press, New York 

  9. Handberg, K. and J. Stougaard 1992 Lotus japonicus, an autogamous, diploid legume species for classical and molecular genetics. Plant J. 2 . 487-496 

  10. Harrison, M. J 2000 Molecular genetics of model legumes. Trends Plant Sci 5 414-415 

  11. Jones, J D. G., L. Shlumukov, F. Carland, J English, S. R. Scofield, G. J. Bishop, and K Harrison. 1992 Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Res 1 : 285-297 

  12. Journet, E. P., M. Pichon, A Dedieu, F de Billy, G. Truchet, and D. G Barker 1994 Rhizobium meliloti Nod factors elicit cell-specific transcription of the Enod12 gene in transgenic alfalfa. Plant J 6 . 241-249 

  13. Li, X., Y Zhang, J . D. Clarke, Y Li, and X Dong 1999. Identification and cloning of a negative regulator of systemic acquired resistance, SNI1, through a screen for suppressors of nprl-1. Cell 98 329-339 

  14. Luehrsen, K R., J. R. de Wet, and V. Walbot 1992. Transient expression analysis in plants using firefly luciferase reporter gene. Methods Enzymol. 216 : 397-414 

  15. Mylona, P., K. Pawlowski, and T. Bisseling. 1995. Symbiotic nitrogen fixation Plant Cell 7 : 869-885 

  16. Nam, Y-W, R. V. Penmetsa, G Endre, P Uribe, D. Kim, and D. R. Cook 1999. Construction of a bacterial artificial chromosome Iibrary of Medicago truncatula and identification of clones for ethylene responsive genes Theor Appl. Genet 98 . 638-646 

  17. Penmetsa, R. V. and D R. Cook. 1997. A legume ethylene-insensitive mutant hyperinfected by Its rhizobial symbiont. Science 275 : 527-530 

  18. Penmetsa, R. V and D. R. Cook 2000 Production and characterization of diverse developmental mutants of Medicago truncatula. Plant Physiol. 123 . 1387-1397 

  19. Scheres, B., F van Engelen, E van der Knaap, C. van de Weil, A van Kammen, and T. Bisseling. 1990. Sequential induction of nodulin gene expression in the developing pea nodule Plant Cell 2 : 687-700 

  20. Thoquet, P., M. Gherardi, E.-P. Journet, A. Kereszt, J.-M. Ane, J M. Prosperi, and T Huguet 2002. The molecular genetic linkage map of the model legume Medicago truncatula: an essential tool for comparative legume genomics and the isolation of agronomically important genes. BMC Plant BioI 2 : 1-13 

  21. Trieu, A T. and M J. Harrison. 1996. Rapid transformation of Medicago truncatula: regeneration via shoot organogenesis. Plant Cell Rep. 16 6-11 

  22. Trieu, A. T, S H. Burleigh, I V. Kardailsky, I E. MaldonadoMendoza, W. K. Versaw, L. A. Blaylock, H Shin, T. J. Chiou, H. Katagi, G R. Dewbre, D. Weigel, and M J Harrison 2000. Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium Plant J. 22 : 531-541 

  23. VandenBosch, K. A , G. Endre, J. I Hur, J. Moore, P Beremand, H. M. Peng, L ElliS, and C D. Town 2000. Functional genomic analysis of early symbiotic development in Medicago truncatula. Molecular Genetics of Model Legumes, John Innes Institute, England, July 24-28. 

  24. van de Sande, K., K. Pawlowski, I. Czaja, U Wieneke, J. Schell, J Schmidt, R. Walden, M. Matvienko, J Wellink, A van Kammen, H. Franssen, and T. Bisseling. 1996. Modification of phytohormone response by a peptide encoded by ENOD40 of legumes and a nonlegume Science 273 . 370-373 

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