$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

Abstract AI-Helper 아이콘AI-Helper

Oxidative stress has been reported to elevate ceramide level during cell death. The purpose of the present study was to modulate cell death in relation to cellular glutathione (GSH) level and GST (glutathione S-transferase) expression by regulating the sphingolipid metabolism. LLC­PK1 cells were tre...

AI 본문요약
AI-Helper 아이콘 AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

  • The medium was changed 36 h after seeding, and then the cells were pretreated with desipramine, /V-acetylcysteine and ISP서 for 1 h, and were then treated with H2O2 for 3 h. The cultured cells were harvested with either 0.25 M trypsin-EDTA treatment or rubber scraper followed by a brief centrifugation, and cell pellets were saved for further experiments.

데이터처리

  • All values were expressed as a mean ± SD. Differences between treatments were analyzed statistically by unpaired Student's f-test. Differences with p < 0.
본문요약 정보가 도움이 되었나요?

참고문헌 (29)

  1. Cui, J., Engelman, R. M., Maulik, N., and Das, D. K., Role of ceramide in ischemic preconditioning. J. Am. Coll. Surg., 198, 770-777 (2004) 

  2. Cumming, R. C., Lightfood, J., Beard, K., Youssoufian, H., OBrien, P. J., and Buchwald, M., Fanconi anemia group C protein prevents apoptosis in hematopoietic cells through redox regulation of GSTP1. Nat. Med., 7, 814-820 (2001) 

  3. Cutler, R. G., Kelly, J., Storie, K., Pedersen, W. A., Tammara, A., Hatanpaa, K., Troncoso, J. C., and Mattson, M. P., Involvement of oxidative stress-induced abnormalities in ceramide and cholesterol metabolism in brain aging and Alzheimers disease. Proc. Natl. Acad. Sci. U.S.A., 101, 2070-2075 (2004) 

  4. Dbaibo, G. S., Pushkareva, M. Y., Rachid, R. A., Alter, N., Smyth, M. J., Obeid, L. M. and Hannun, Y. A., p53-dependent ceramide response to genotoxic stress. J. Clin. Invest., 102, 329-339 (1998) 

  5. Dobrowsky, R. T., Kamibayashi, C., Mumby, M. C., and Hannun, Y. A., Ceramide activates heterotrimeric protein phosphatase 2A. J. Biol. Chem., 268, 15523-15530 (1993) 

  6. Goldkorn, T., Ravid, T., and Khan, E. M., Life and death decisions: ceramide generation and EGF receptor trafficking are modulated by oxidative stress. Antioxid. Redox. Signal., 7, 119-128 (2005) 

  7. Grassme, H., Jendrossek, V., Bock, J., Riehle, A., and Gulbins E., Ceramide-rich membrane rafts mediate CD40 clustering. J. Immunol., 168, 298-307 (2002) 

  8. Hannun, Y. A. and Luberto, C., Ceramide in the eukaryotic stress response. Cell Biol., 10, 73-80 (2000) 

  9. Hannun, Y. A., Luberto, C., and Argraves, K. M., Enzymes of sphingolipid metabolism: from modular to integrative signaling. Biochemistry, 40, 4893-4903 (2001) 

  10. Jaffrezou, J. P., Bruno, A. P., Moisand, A., Levade, T., and Laurent, G., Activation of a nuclear sphingomyelinase in radiation-induced apoptosis. FASEB J., 15, 123-133 (2001) 

  11. Jensen, J.-M., Folster-Holst, R., Baranowsky, A., Schuck, M., Winoto-Morbach, S., Neumann, C., Schutze, S., and Proksch, E., Impaired sphingomyelinase activity and epidermal differentiation in atopic dermatitis. J. Invest. Dermatol., 122, 1423-1431 (2004) 

  12. Lavrentiadou, S. N., Chan, C., Kawcak, T. N., Ravid, T., Tsaba, A., van der Vliet, A., Rasooly, R., and Goldkorn, T., Ceramidemediated apoptosis in lung epithelial cells is regulated by glutathione. Am. J. Respir. Cell. Mol. Biol., 25, 676-684 (2001) 

  13. Lee, J. T., Xu, J., Lee, J. M., Ku, G., Han, X., Yang, D.I., Chen, S., and Hsu, C. Y., Amyloid- $\beta$ induces oligodendrocyte death by activating the neutral sphingomyelinase-ceramide pathway. J. Cell. Biol., 164, 123-131 (2004) 

  14. Mathias, S., Dressler, K. A., and Kolesnick, R. N., Characterization of a ceramide-activated protein kinase: stimulation by tumor necrosis factor $\alpha$ . Proc. Natl. Acad. Sci. U.S.A., 88, 10009-10013 (1991) 

  15. Mathias, S., Pena, L. A., and Kolesnick, R. N., Signal transduction of stress via ceramide. Biochem. J., 335, 465-480 (1998) 

  16. Mihm, S., Ennen, J., Pessara, U., Kurth, R., and Dorge, W., Inhibition of HIV-1 replication and NF-kappa B activity by cysteine and cysteine derivatives. AIDS, 5, 497-503 (1991) 

  17. Morceau, F., Duvoix A., Delhalle, S., Schnekenburger, M., Dicato, M., and Diederich, M., Regulation of glutathione Stransferase P1-1 gene expression by NF-kappaB in tumor necrosis factor alpha-treated K562 leukemia cells. Biochem. Pharmacol., 67, 1227-1238 (2004) 

  18. Papp, S., Robaire, B., and Hermo, L., Immunocytochemical localization of the Ya, Yc, Yb1, and Yb2 subunits of glutathione S-transferases in the testis and epididymis of adult rats. Microsc. Res. Tech., 30, 1-23 (1995) 

  19. Pickett, C. B. and Lu, A. Y., Glutathione S-transferases: gene structure, regulation, and biological function. Annu. Rev. Biochem., 58, 743-764. (1989) 

  20. Rao, A. V. and Shaha, C., Role of glutathione S-transferases in oxidative stress-induced male germ cell apoptosis. Free Radic. Biol. Med., 29, 1015-1027 (2000) 

  21. Salinas, A. E. and Wong, M. G., Glutathione S-transferases - a review. Curr. Med. Chem., 6, 279-309 (1999) 

  22. Santana, P., Pena, L. A., Haimovitz-Friedman, A., Martin, S., Green, D., McLoughlin, M., Cordon-Cardo, C., Schuchman, E. H., Fuks, Z., and Kolesnick, R., Acid sphingomyelinasedeficient human lymphoblasts and mice are defective in radiation-induced apoptosis. Cell, 86, 189-199 (1996) 

  23. Schreck, R., Rieber, P., and Baeuerle, P. A., Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1. EMBO J., 10, 2247-2258 (1991) 

  24. Schulze-Osthoff, K., Beyaert, R., Vandevoorde, V., Haegeman, G., and Fiers, W., Depletion of the mitochondrial electron transport abrogates the cytotoxic and gene-inductive effects of TNF. EMBO J., 12, 3095-3104 (1993) 

  25. Sugiura, M., Kono, K., Liu, H., Shimizugawa, T., Minekura, H., Spiegel, S., and Kohama, T., Ceramide kinase, a novel lipid kinase. J. Biol. Chem., 277, 23294-23300 (2002) 

  26. Wasserman, W. W. and Fahl, W. E., Functional antioxidant responsive elements. Proc. Natl. Acad. Sci. U.S.A., 94, 5361- 5366 (1997) 

  27. Yang, Y., Cheng, J. Z., Singhal, S. S., Saini, M., Pandya, U., Awasthi, S., and Awasthi Y. C., Role of glutathione Stransferases in protection against lipid peroxidation. Overexpression of hGST A2-2 in k562 cells protects against hydrogen peroxide-induced apoptosis and inhibits JNK and caspase 3 activation. J. Biol. Chem., 276, 19220-19230 (2001) 

  28. Yin, Z., Ivanov, V. N., Habelhah, H., Tew, K., and Ronai, Z., Glutathione S-transferase P elicits protection against $H_2O_2$ -induced cell death via coordinated regulation of stress kinases. Cancer Res., 60, 4053-4057 (2000) 

  29. Yu, M. U., Yoo, J. M., Lee, Y. M., Hong, J. T., Oh, K. W., Song, S. K., Yun, Y. P., Yoo, H. S., and Oh, S., Altered de novo sphingolipid biosynthesis is involved in the serumdeprivation- induced cell death in LLC-PK1 cells. J. Toxicol. Environ. Health A, 67, 2085-2094 (2004) 

저자의 다른 논문 :

관련 콘텐츠

오픈액세스(OA) 유형

FREE

Free Access. 출판사/학술단체 등이 허락한 무료 공개 사이트를 통해 자유로운 이용이 가능한 논문

섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트