[논문]Staphylococcus aureus Siderophore-Mediated Iron-Acquisition System Plays a Dominant and Essential Role in the Utilization of Transferrin-Bound Iron

Park Ra Young; Sun Hui Yu; Choi Mi Hwa; Bai Young Hoon; Shin Sung Heui

Staphylococcus aureus Siderophore-Mediated Iron-Acquisition System Plays a Dominant and Essential Role in the Utilization of Transferrin-Bound Iron 원문보기

The journal of microbiology, v.43 no.2, 2005년, pp.183 - 190

Park Ra Young (Research Center for Resistant Cells) , Sun Hui Yu (Research Center for Resistant Cells) , Choi Mi Hwa (Research Center for Resistant Cells) , Bai Young Hoon (Department of Biology, Chosun University) , Shin Sung Heui (Research Center for Resistant Cells)

Abstract ▼ AI-Helper

Staphylococcus aureus is known to be capable of utilizing transferrin-bound iron, via both siderophoreand transferrin-binding protein (named IsdA)-mediated iron-acquisition systems. This study was designed in order to determine which iron-acquisition system plays the essential or dominant role with respect to the acquisition of iron from human transfenin, in the growth of S. aureus. Holotransferrin (HT) and partially iron-saturated transferrin (PT), but not apotransferrin (AT), were found to stimulate the growth of S. aureus. S. aureus consumed most of the transferrin-bound iron during the exponential growth phase. Extracellular proteases were not, however, involved in the liberation of iron from transferrin. Transferrin-binding to the washed whole cells via IsdA was not observed during the culture. The expression of IsdA was observed only in the deferrated media with AT, but not in the media supplemented with PT or HT. In contrast, siderophores were definitely produced in the deferrated media with PT and HT, as well as in the media supplemented with AT. The siderophores proved to have the ability to remove iron directly from transferrin, but the washed whole cells expressing IsdA did not. In the bioassay, the growth of S. aureus on transferrin-bound iron was stimulated by the siderophores alone. These results demonstrate that the siderophore-mediated iron-acquisition system plays a dominant and essential role in the uptake of iron from transferrin, whereas the IsdA-mediated iron-acquisition system may play only an ancillary role in the uptake of iron from transferrin.

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가설 설정

5 mg/ml of holotransferrin at 37°C for 0, 10, 30 and 60 min. D: The culture ultrafiltrate with siderophore activity (B) was allowed to react with holotransferrin under the similar conditions. After the completion of the reaction, 6 M urea-gel electrophoresis was performed.

제안 방법

About 1 x 106 cfu/ml of this preconditioned bacteri_니m was inoculated into the test media, and cultured with vigorous shaking (220 rpm) at 37°C for 24 h. At appro priate intervals, culture fluids were withdrawn and used to monitor bacterial growth, via the measurement of optical densities at a wavelength of 600 nm (OD600), and the culture supernatants, which were obtained by centrifugation (10, 000 rpm, 5 min) at the same intervals, were used in several assays performed in this study.
In order to observe whether the growth of S. aureus could be stimulated using only siderophores, bioassays using the ultrafiltrates of the cultures exhibiting siderophore activity were performed. According to oulr observations, the growth of S.
No significant differences were noted between PT and HT in the DF-BHI, and this held true for _나le MM9 as well. Therefore, further experiments in this study were carried out in DF-BHI, as the growth of S. aureus in the MM9 containing AT was so severely retarded that the produc tion of siderophores and the expression of transferrin- binding protein were below our experimental threshold of detection. In order to observe the utilization of transferrin- bound iron, eq_니al volumes (20 #1) of the culture super natants were electrophoresed on 6 M urea gel (Fig.

이론/모형

The supernatants (20 #1) were elec trophoresed on 6 M urea-gel. The siderophore activity of the culture supernatant was verified by CAS agar diffii- sion assay (Fig. 5B). The culture supernatant was then ultrafiltrated (Vvaspin, MWCO 10, 000, Sartorius, Ger many) in order to obtain the crude fraction of the low- molecular-weight siderophores.

성능/효과

They reported that the removal of iron from transferrin occurred more rapidly when completed by sta- phyloferrin A, a siderophore produced by staphylococci, than by the washed whole cells. Overall, these results dem onstrated that the siderophore-mediated iron-acquisition system plays a dominant or essential role in the uptake of iron from transferrin, whereas the IsdA-mediated iron- acqulisition system, if it is involved at all, plays only an ancillary role in the uptake of iron from transferrin.
2004), it was demonstrated that both the expression of IsdA and the production of siderophores were iron-repressible, indicating Fur regulation (Xiong et 4 2000). Therefore, our results indicated that concen trations of intracellular iron increased as a result of the siderophore-mediated iron acquisition system, which played an essential and dominant role in iron-uptake from transferrin, and the increased intracellular iron concentra tions repressed the expression of IsdA via Fur regulation in the DF-BHI containing PT and HT.
Similar results were observed, even in the MM9 containing AT as well as HT (data not shown). These results indicated that S. aureus possesses the ability to utilize transferrin-bound iron for growth via only the siderophore-mediated iron-acquisition system, and completely independently of the IsdA-mediated iron- acquisition system.

참고문헌 (43)

Andrews, S.C., A.K. Robinson, and F. Rodriguez-Quinones. 2003. Bacterial iron homeostasis. FEMS Microbiol. Rev. 27, 215-237

상세보기
Braun, V. and M. Barun. 2002. Active transport of iron and siderophore antibiotics. Curr. Opin. Microbiol. 5, 194-201

상세보기
Brock, J.H., P.H. Williams, J. Liceaga, and K.G. Woolridge. 1991. Relative availability of transferrin-bound iron and cell-driven iron to aerobactin-producing and enterochelin-producing strains of Escherichia coli and to other microorganisms. Infect. Immun. 59, 3185-3190
Brown, J.S. and D.W. Holden. 2002. Iron acquisition by Gram-positive bacterial pathogens. Microbes Infect. 4, 1149-1156

상세보기
Bullen, J., E. Griffiths, H. Rogers, and G. Ward. 2002. Sepsis: the critical role of iron. Microbes Infect. 2, 409-415

상세보기
Cabrera, G., A. Xiong, M. Uebel, V.K. Singh, and R.K. Jayaswal. 2001. Molecular characterization of the iron-hydroxamate uptake system in Staphylococcus aureus. Appl. Environ. Microbiol. 67, 1001-1003

상세보기
Clarke, S.R., M.D. Wiltshire, and S.J. Foster. 2004. IsdA of Staphylococcus aureus is a broad spectrum, iron-regulated adhesion. Mol. Microbiol. 51, 1509-1519

상세보기
Conrad, M.E. 2001. Iron: seminal publications of the twentieth century-transferrin. J. Tra. Ele. Exp. Med. 14, 115-117

상세보기
Courcol, R.J., D. Trivier, M.C. Bissinger, G.R. Martin, and M.R. Brown. 1997. Siderophore production by Staphylococcus aureus and identification of iron-regulated proteins. Infect. Immun. 65, 1944-1948

상세보기
Crosa, J .H. 1997. Signal transduction and transcriptional and posttranscriptional control of iron-regulated genes in bacteria. Microbiol. Mol. Biol. Rev. 61, 319-336

상세보기
Dale, S.E., A. Doherty-Kirby, G. Lajoie and D.E. Heinrichs. 2004. Role of siderophore biosynthesis in virulence of Staphylococcus aureus: Identification and characterization of genes involved in production of a siderophore. Infect. Immun. 72, 29-37

상세보기
Drechsel, H., S. Freund, G. Nicholson, H. Haag, O. Jung, H. Zahner, and G. Jung. 1993. Purification and chemical characterization of staphyloferrin B, a hydrophilic siderophore from staphylococci. Biometals 6, 185-192

상세보기
Dryla, A., D. Gelbmann, A. Von Gabain, and E. Nagy. 2003. Identification of a novel iron regulated staphylococcal surface protein with haptoglobulin-hemoglobin binding activity. Mol. Microbiol. 49, 37-53

상세보기
Emori, T.G and R.P. Gaynes. 1993. An overview of nosocomial infections, including the role of the microbiology laboratory. Clin. Microbiol. Rev. 6, 428-442

상세보기
Konetschny-Rapp, S., G. Jung, J. Meiwes, and H. Zahner. 1990. Staphyloferrin A: a structurally new siderophore from staphylococci. Eur. J. Biochem. 191, 65-74

상세보기
Leong, S.A. and J.B. Neilands. 1982. Siderophore production by phytopathogenic microbial species. Arch. Biochem. Biophys. 218, 351-359

상세보기
Lim, Y., S.H. Shin, I.Y. Jang, J.H. Rhee, and I.S. Kim. 1998. A human transferrin-binding protein of Staphylococcus aureus is immunogenic in vivo and has an epitope in common with human transferrin receptor. FEMS Microbiol. Lett. 166, 225-230

상세보기
Lim, Y., S.H. Shin, S.I. Lee, I.S. Kim, and J.H. Rhee. 1998. Ironrepressibility of siderophore and transferrin-binding protein in Staphylococccus aureus. FEMS Microbiol. Lett. 163, 19-24

상세보기
Lim, Y., S.Y. Cho, N.S. Cho, S.I. Lee, J.Y. Chung, C.H. Chung, and S.H. Shin. 2004. Oxygen stimulates the expression of ironrepressible high-affinity iron-uptake systems of Staphylococcus aureus-Application of CAS agar diffusion assay. Infection and Chemotherapy 36, 32-39
Lindsay, J.A. and T.V. Riley. 1994. Staphylococcal iron requirements, siderophore production, and iron-regulated protein expression. Infect. Immun. 62, 2309-2314
Lindsay, J.A., T.V. Riley, and B.J. Mee. 1995. Staphylococcus aureus but not Staphylococcus epidermidis can acquire iron from transferrin. Microbiology 141, 197-203

상세보기
Makey, D.G. and U.S. Seal. 1988. The detection of four molecular forms of human transferrin during the iron binding process. Biochem. Biophys. Acta 453, 250-256
Martinaho, S., L. von Bonsdorff, A. Rouhiainen, M. Lonnroth and J. Parkkinen. 2001. Dependence of Staphylococcus epidermidis on non transferrin-bound iron for growth. FEMS Microbiol. Lett. 196, 177-182

상세보기
Mazmanian, S.K., E.P. Skaar, A.H. Gaspar, M. Humayun, P. Gornicki, J. Jelenska, A. Joachmiak, D.M. Missiakas, and O. Schneewind. 2003. Passage of heme-iron across the envelope of Staphylococcus aureus. Science 299, 906-909

상세보기
Modun, B.J., A. Cockayne, R. Finch, and P. Williams. 1998. The Staphylococcus aureus and Staphylococcus epidermidis transferrin-binding proteins are expressed in vivo during infection. Microbiology 144, 1005-1012

상세보기
Modun, B., D. Kendall, and P. Williams. 1994. Staphylococci express a receptor for human transferrin: Identification of a 42-kilodalton cell wall transferrin-binding protein. Infect. Immun. 62, 3850-3858

상세보기
Modun, B. and P. Williams. 1999. The staphylococcal transferrinbinding protein is a cell wall glyceraldehydes-3-phosphate dehydrogenase. Infect. Immun. 67, 1086-1092
Modun, B., R.W. Evans, C.L. Joannou, and P. Williams. 1998. Receptor-mediated recognition and uptake of iron from human transferrin by Staphylococcus aureus and Staphylococcus epidermidis. Infect. Immun. 66, 3591-3596

상세보기
Morrissey, J.A., A. Cockayne, P.J. Hill, and P. Williams. 2000. Molecular cloning and analysis of a putative siderophore ABC transporter from Staphylococcus aureus. Infect. Immun. 68, 6281-6288

상세보기
Okuzo, N., T. Akiyama, S. Miyoshi, S. Shinoda, and S. Yamamoto. 1996. Involvement of vulnibactin and exocellular protease in utilization of transferrin-and lactoferrin-bound iron by Vibrio vulnificus. Microbiol. Immunol. 40, 595-598

상세보기
Park, R.Y., H.Y. Sun, M.H. Choi, Y.H. Bae, and S.H. Shin. 2005. Growth of Staphylococcus aureus with defective siderophore production in human peritoneal dialysate solution. J. Microbiol. 43, 54-61

원문보기 상세보기
Potempa, J., A. Dubin, G. Korzus, and J. Travis. 1988. Degradation of elastin by a cysteine proteinase from Staphylococcus aureus. J. Biol. Chem. 263, 2664-2667

상세보기
Ratledge, C. and L. Dover. 2000. Iron metabolism in pathogenic bacteria, Ann. Rev. Microbiol. 54, 881-941

상세보기
Rybak, M.J. and R.L. Akins. 2001. Emergence of methicillin-resistant Staphylococcus aureus with intermediate glycopeptide resistance: clinical significance and treatment options. Drugs 61, 1-7
Schwyn, B. and J.B. Neilands. 1987. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160, 47-56

상세보기
Sebulsky, M.T. and D.E. Heinrichs. 2001. Identification and characterization of fhuD1 and fhuD2, two genes involved in ironhydroxamate uptake in Staphylococcus aureus. J. Bacteriol. 183, 4994-5000

상세보기
Sebulsky, M.T., D. Hohnstein, M.D. Hunter, and D.E. Heinrichs. 2000. Identification and characterization of a membrane permease involved in iron-hydroxamate transport in Staphylococcus aureus. J. Bacteriol. 182, 4394-4400

상세보기
Shin, S.H., Y. Lim, S.E. Lee, N.W. Yang, and J.H. Rhee. 2001. CAS agar diffusion assay for the measurement of siderophores in biological fluids. J. Microbiol. Methods 44, 89-95

상세보기
Sieradzki, K., R.B. Roberts, S.W. Haber, and A.Tomasz. 1999. The development of vancomycin resistance in a patient with methicillin- resistant Staphylococcus aureus infection. N. Engl. J. Med. 340, 517-523

상세보기
Skaar, E.P. and O. Schneewind. 2004. Iron-regulated surface determinants (Isd) of Staphylococcus aureus: stealing iron from heme. Microbes Infect. 6, 390-397

상세보기
Taylor, J.M. and Heinrichs, D.E. (2002) Transferrin binding in Staphylococcus aureus: involvement of a cell wall-anchored protein. Mol. Microbiol. 43, 1603-1614

상세보기
Trivier, D. and R.J. Courcol. 1996. Iron depletion and virulence in Staphylococcus aureus. FEMS Microbiol. Lett. 141, 117-127

상세보기
Xiong, A., V.K. Singh, G. Cabrera, and R.K. Jayaswal. 2000. Molecular characterization of the ferric-uptake regulator, Fur, from Staphylococcus aureus. Microbiology 146, 659-668

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