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Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium

The journal of microbiology, v.43 no.2, 2005년, pp.204 - 208  

Yoo So Young (Department of Oral Biochemistry, College of Dentistry, Chosun University) ,  Kim Pyung Sik (Department of Conservative Dentistry, College of Dentistry, Chosun University) ,  Hwan Ho Keel (Department of Conservative Dentistry, College of Dentistry, Chosun University) ,  Lim Seong Hoon (Department of Orthodontics, College of Dentistry, Chosun University) ,  Kim Kwang Won (Department of Orthodontics, College of Dentistry, Chosun University) ,  Choe Son Jin (Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University) ,  Min Byung Moo (Department of Oral Biochemistry, College of Dentistry, Seoul National University) ,  Kook Joong Ki (Department of Oral Biochemistry, College of Dentistry, Seoul National University)

Abstract AI-Helper 아이콘AI-Helper

The objective of this study was to both isolate and identify non-mutans streptococci organisms (non­MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on MSB....

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제안 방법

  • The development of an orange-yellow color indicated the generation of ammonia. These biochemical tests were repeated with cultures of the reference strains, in order to determine reproducibility and reliability.

대상 데이터

  • Ethical approval for this study was granted by the Insti­ tutional Research Board of Chosun University. 63 patients, aged 14 to 34 years (average age was 16 years) were enrolled in this study. None of the participants had received antibiotic therapy in the prior 3 months, and none had any history of systemic disease.

이론/모형

  • The PCR product was then purified with an AccuPrep® PCR purification kit (Bioneer, Korea), and was directly ligated with pEZ-T Easy vector (RNA, Korea). 16S rDNA nucle­ otide sequences were determined using the dideoxy chain termination method, with a Big Dye Terminator Cycle Sequencing Kit (Applied Biosystems, USA) and an ABI PRISM 310 Genetic Analyzer (Applied Biosystems, USA). The primers used for nucleotide sequencing were as fol­ lows: ChDC-F (5'-AAT ACG ACT CAC TAT AGG GCG AA-3'), ChDC-R (5'-CCT CAC TAA AGG GAA CAA AAG C-3‘), Seq-Fl (5'-CCT ACG GGA GGC AGC AG­ S'), Seq-R2 (5'-GAC TAC CAG GGT ATC TAA TCC-3'), and F16 (5'-TAG ATA CCC YGG TAG TCC-3').
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참고문헌 (16)

  1. Emilson, C.G. 1983. Prevalence of Streptococcus mutans with different colonial morphologies in human plaque and saliva. Scand. J. Dent. Res. 91, 26-32 

  2. Farmer III, J.J. 1999. Enterobacteriaceae: Introduction and identification, p. 442-458. In P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (eds.), Manual of clinical microbiology. American Society for Microbiology, Washington, D.C 

  3. Gold, O.G., H.V. Jordan, and J. van Houte. 1973. A selective medium for the isolation of Streptococcus mutans. Arch. Oral Biol. 18, 1357-1364 

  4. Hirasawa, M. and K. Takada. 2002. Susceptibility of Streptococcus mutans and Streptococcus sobrinus to cell wall inhibitors and development of a novel selective medium for S. sobrinus. Caries Res. 36, 155-160 

  5. Hirasawa, M. and K. Takada. 2003. A new selective medium for Streptococcus mutans and the distribution of S. mutans and S. sobrinus and their serotypes in dental plaque. Caries Res. 37, 212-217 

  6. Jensen, B. and D. Bratthall. 1989. A new method for the estimation of mutans streptococci in human saliva. J. Dent. Res. 68, 468-471 

  7. Kimmel, L. and N. Tinanoff. 1991. A modified mitis salivarius medium for a caries diagnostic test. Oral Microbiol. Immunol. 6, 275-279 

  8. Krasse, B. 1966. Human streptococci and experimental caries in hamsters. Arch. Oral Biol. 11, 429-436 

  9. Lane, D.J. 1991. 16S/23S rRNA sequencing, p. 115-175. In E. Stackebrandt, and M. Goodfellow (eds.), Nucleic acid techniques in bacterial systematics. John Wiley & Sons, Inc., New York 

  10. Loesche, W.J. 1986. Role of Streptococcus mutans in human dental decay. Microbiol. Rev. 50, 353-380 

  11. Niven, Jr., C.F., K.L. Smiley, and J.M. Sherman. 1942. The hydrolysis of arginine by streptococci. J. Bacteriol. 43, 651-660 

  12. Shklair, I.L. and H.J. Keene. 1974. A biochemical scheme for the separation of the five varieties of Streptococcus mutans. Arch. Oral Biol. 19, 1079-1081 

  13. Shklair, IL. and H.J. Keene. 1976. Biochemical characterization and distribution of Streptococcus mutans in three diverse populations, p. 201-210. In H.M. Stiles, W.J. Loesche, and T.C. O'Brien (eds.), Microbial aspects of dental caries. Information Retrieval Inc., Washington, D.C 

  14. Tanzer, J.M., A.C. Borjesson, L. Laskowski, A.B. Kurasz, and M. Testa. 1984. Glucose-sucrose-potassium tellurite-bacitracin agar, an alternative to mitis salivarius-bacitracin agar for enumeration of Streptococcus mutans. J. Clin. Microbiol. 20, 653-659 

  15. Van Palenstein Helderman, W.H., M. Ijsseldijk, and J.H. Huis in 't Veld. 1983. A selective medium for the two major subgroups of the bacterium Streptococcus mutans isolated from human dental plaque and saliva. Arch. Oral Biol. 28, 599-603 

  16. Whiley, R.A. and D. Beighton. 1998. Current classification of the oral streptococci. Oral Microbiol. Immunol. 13, 195-216 

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