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The objective of this study was to both isolate and identify non-mutans streptococci organisms (non­MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects, 12 colonies, derived from 10 subjects, were identified as non-MSO. These 12 colonies were determined to be Streptococcus anginosus (8 colonies), S. sanguinis (1 colony), and Pantoea agglomerans (3 colonies). These results strongly suggest that a new selective medium will be required for the reliable isolation of mutans streptococci.

참고문헌 (16)

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  4. Van Palenstein Helderman, W.H., M. Ijsseldijk, and J.H. Huis in 't Veld. 1983. A selective medium for the two major subgroups of the bacterium Streptococcus mutans isolated from human dental plaque and saliva. Arch. Oral Biol. 28, 599-603 
  5. Farmer III, J.J. 1999. Enterobacteriaceae: Introduction and identification, p. 442-458. In P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (eds.), Manual of clinical microbiology. American Society for Microbiology, Washington, D.C 
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  11. Shklair, IL. and H.J. Keene. 1976. Biochemical characterization and distribution of Streptococcus mutans in three diverse populations, p. 201-210. In H.M. Stiles, W.J. Loesche, and T.C. O'Brien (eds.), Microbial aspects of dental caries. Information Retrieval Inc., Washington, D.C 
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  13. Krasse, B. 1966. Human streptococci and experimental caries in hamsters. Arch. Oral Biol. 11, 429-436 
  14. Hirasawa, M. and K. Takada. 2003. A new selective medium for Streptococcus mutans and the distribution of S. mutans and S. sobrinus and their serotypes in dental plaque. Caries Res. 37, 212-217 
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이 논문을 인용한 문헌 (5)

  1. 2006. "" International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute, 31(1): 7~10 
  2. Choi, Min-Ho ; Yoo, So-Young ; Lim, Chae-Kwang ; Kang, Dong-Wan ; Kook, Joong-Ki 2006. "Nested PCR for the Detection of Streptococcus mutans" Korean journal of microbiology = 미생물학회지, 42(1): 19~25 
  3. 2007. "" The journal of microbiology, 45(3): 246~255 
  4. Jeong, Hyun-Ja ; Kim, Su-Jung 2010. "Distribution and Antimicrobial Susceptibility of Bacteria in the Oral Cavity of Smokers or Non-Smokers" Korean journal of microbiology = 미생물학회지, 46(4): 334~340 
  5. 2010. "" International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute, 35(1): 21~25 


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