• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


The purpose of this study was to develop species-specific PCR primers for use in the identification and detection of Actinobacillus actinomycetemcomitans. These primers target variable regions of the 168 ribosomal RNA coding gene (rDNA). We assessed the specificity of the primers against 9 A. actinomycetemcomitans strains and 11 strains (3 species) of the Haemophilus genus. Primer sensitivity was determined by testing serial dilutions of the purified genomic DNAs of A. actinomycetemcomitans ATCC$ 33384^$T Our obtained data revealed that we had obtained species-specific amplicons for all of the tested A. actinomycetemcomitans strains, and that none of these amplicons occurred in any of the other species. Our PCR protocol proved able to detect as little as 4 fg of A. actinomycetemcomitans chromosomal DNA. Our findings suggest that these PCR primers are incredibly sensitive, and should prove suitable for application in epidemiological studies, as well as the diagnosis and monitoring of periodontal pathogens after treatment for periodontitis.

참고문헌 (21)

  1. Conrads, G., T.F. Flemmig, I. Seyfarth, F. Lampert, and R. Lutticken. 1999. Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR. J. Clin. Microbiol. 37, 1621-1624 
  2. Lally, E.T., I.R. Kieba, D.R. Demuth, J. Rosenbloom, E.E. Golub, N.S. Taichman, and C.W. Gibson. 1989. Identification and expression of the Actinobacillus actinomycetemcomitans leukotoxin gene. Biochem. Biophys. Res. Commun. 159, 256-262 
  3. Seong, J.H., D.K. Kim, K.Y. Shin, B.O. Kim, M.S. Kim, M.K. Kim, and J.K. Kook. 2003. Simultaneous detection of Prevotella intermedia and Prevotella nigrescens by 16S rDNA-directed multiplex PCR. J. Korean Acad. Dent. Health 26, 523-533 
  4. Tran, S.D. and J.D. Rudney. 1999. Improved multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis. J. Clin. Microbiol. 37, 3504-3508 
  5. Asikainen, S. 1986. Occurrence of Actinobacillus actinomycetemcomitans and spirochetes in relation to age in localized juvenile periodontitis. J. Periodontol. 57, 537-541 
  6. Kook, J.-K., M.-K. Kim, J.-H. Seong, D.-K. Kim, B.-O. Kim, J.-C. Park, K.-K. Kim, S.-J. Choe, and B.-M. Min. 2003. A new method for rapid screening of bacterial species- or subspeciesspecific DNA probes. FEMS Microbiol. Lett. 219, 121-127 
  7. Zambon, J.J. 1985. Actinobacillus actinomycetemcomitans in human periodontal disease. J. Clin. Periodontol. 12, 1-20 
  8. Robertson, P.B., M. Lantz, P.T. Marucha, K.S. Kornman, C.L. Trummel, and S.C. Holt. 1982. Collagenolytic activity associated with Bacteroides species and Actinobacillus actinomycetemcomitans. J. Periodontal Res. 17, 275-283 
  9. Ashimoto, A., C. Chen, I. Bakker, and J. Slots. 1996. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol. Immunol. 11, 266-273 
  10. Tonjum, T., and R. Haas. 1993. Identification of Actinobacillus actinomycetemcomitans by leukotoxin gene-specific hybridization and polymerase chain reaction assays. J. Clin. Microbiol. 31, 1856-1859 
  11. Darveau, R.P., A. Tanner, and R.C. Page. 1997. The microbial challenge in periodontitis. Periodontol. 2000 14, 12-32 
  12. Kiley, P. and S.C. Holt. 1980. Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27. Infect. Immun. 30, 862-873 
  13. Lippke, J.A., M.N. Strzempko, F.F. Raia, S.L. Simon, and C.K. French. 1987. Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate. Appl. Environ. Microbiol. 53, 2588-2589 
  14. Ashkenazi, M., R.R. White, and D.K. Dennison.1992. Neutrophil modulation by Actinobacillus actinomycetemcomitans. I. Chemotaxis, surface receptor expression and F-actin polymerization. J. Periodontal Res. 27, 264-273 
  15. Slots, J., A. Ashimoto, M.J. Flynn, G. Li, and C. Chen. 1995. Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction. Clin. Infect. Dis. 20(Suppl 2), S304-307 
  16. Mandell, R.L. 1984. A longitudinal microbiological investigation of Actinobacillus actinomycetemcomitans and Eikenella corrodens in juvenile periodontitis. Infect. Immun. 45, 778-780 
  17. Sugai, M., T. Kawamoto, S.Y. Peres, Y. Ueno, H. Komatsuzawa, T. Fujiwara, H. Kurihara, H. Suginaka, and E. Oswald. 1998. The cell cycle-specific growth-inhibitory factor produced by Actinobacillus actinomycetemcomitans is a cytolethal distending toxin. Infect. Immun. 66, 5008-5019 
  18. Woese, C.R. 1987. Bacterial evolution. Microbiol. Rev. 51, 221-271 
  19. van Winkelhoff, A.J. and J. Slots. 1999. Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in nonoral infections. Periodontol. 2000 20, 122-135 
  20. Tran, S.D. and J.D. Rudney. 1996. Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. J. Clin. Microbiol. 34, 2674-2678 
  21. Genco, R.J., J.J. Zambon, and P.A. Murray. 1985. Serum and gingival fluid antibodies as adjuncts in the diagnosis of Actinobacillus actinomycetemcomitans-associated periodontal disease. J. Periodontol. 56, 1-50 

이 논문을 인용한 문헌 (3)

  1. Choi, Min-Ho ; Yoo, So-Young ; Lim, Chae-Kwang ; Kang, Dong-Wan ; Kook, Joong-Ki 2006. "Nested PCR for the Detection of Streptococcus mutans" Korean journal of microbiology = 미생물학회지, 42(1): 19~25 
  2. 2007. "" The journal of microbiology, 45(3): 246~255 
  3. 2011. "" International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute, 36(1): 1~6 


원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일