$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"

과학기술 지식인프라 ScienceON

상세검색 다국어 입력
도움말도움말
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

MyON담기
내보내기

Molecular Taxonomy of a Soil Actinomycete Isolate, KCCM10454 Showing Neuroprotective Activity by 16S rRNA and rpoB Gene Analysis

The journal of microbiology v.43 no.2 , 2005년, pp.213 - 218  
Lee Bong Hee (Department of Anatomy & Neurobiology, Cheju National University ) ;  Kim Hong ( Department of Microbiology, and Liver Research Institute, College of Medicine, Seoul National University ) ;  Kim Hyun Ju ( Department of Microbiology, and Liver Research Institute, College of Medicine, Seoul National University ) ;  Lim Yoon Kyu ( Department of Veterinary Public Health, College of Veterinary Medicine, Cheju National University ) ;  Byun Kyung Hee ( Department of Anatomy & Neurobiology, Cheju National University ) ;  Hutchinson Brian ( Department of Anatomy & Neurobiology, Cheju National University ) ;  Kim Chang Jin ( Korea Research institute of Bioscience and Biotechnology ) ;  Ko Young Hwan ( Department of Food Science and Engineering, Cheju National University ) ;  Lee Keun Hwa ( Department of Microbiology, College of Medicine, Institute of Medical Science, Cheju National University ) ;  Cha Chang Yong ( Department of Microbiology, and Liver Research Institute, College of Medicine, Seoul National University ) ;  Kook Yoon Hoh ( Department of Microbiology, and Liver Research Institute, College of Medicine, Seoul National University ) ;  Kim Bum Joon ( Department of Microbiology, and Live)
Abstract

Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampinresistant genotype, Asn$(AAC)^$442, according to the results of 16S rRNA and rpoB gene analyses

저자의 다른 논문

김홍(2) 김창진(27) Ko, Young-Hwan(26) 차창용(25) 국윤호(20) 김범준(13)

참고문헌 (14)

  1. Kim, B.J., M. Cho, J.C. Kim, K.Y. Cho, G.J. Choi, C.H. Lee, and Y. Lim. 2001. Streptomyces showing antifungal activities against six plant pathogenic fungi. J. Microbiol. Biotechnol. 11, 1120-1123 
  2. Kim, B.J., Y.H. Koh, J. Chun, C.J. Kim, S.H. Lee, M. Cho, J.W. Hyun, K.H. Lee, C.Y. Cha, and Y.H. Kook. 2003. Differentiation of actinomycete genera based on partial rpoB gene sequences. J. Microbiol. Biotechnol. 13, 846-852 
  3. Kirschner, P., M. Kiekenbeck, D. Meissner, J. Wolters, and E.C. Bottger. 1992. Genetic heterogeneity within Mycobacterium fortuitum complex species: genotypic criteria for identification. J. Clin. Microbiol. 30, 2772-2775 
  4. Maher, P. and Schubert, D. 2000. Signaling by reactive oxygen species in the nervous system. Cell. Mol. Life Sci. 57, 1287-1305 
  5. Mattson, M.P. 2000. Apoptosis in neurodegenerative disorders. Nat. Rev. Mol. Cell. Biol. 1, 120-129 
  6. Bozzi, Y., D. Vallone, and E. Borrelli. 2000. Neuroprotective role of dopamine against hippocampal cell death. J. Neurosci. 20, 8643-8649 
  7. Kumar, S., K. Tamura, I.B. Jakobsen, and M. Nei. 2001. MEGA2: Molecular Evolutionary Genetics Analysis software, Arizona State University, Tempe, Arizona, USA 
  8. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783-791 
  9. Lobner, D. 2000. Comparison of the LDH and MTT assays for quantifying cell death: validity for neuronal apoptosis? J. Neurosci. Methods. 96, 147-152 
  10. Kim, B.J., G.J. Choi, K.Y. Cho, H. Yang, C. Shin, C.H. Lee, and Y. Lim. 2002. Antifungal activities against Plasmodiophora brassicae causing club root. J. Microbiol. Biotechnol. 12, 1122-1125 
  11. Morrison, R.S., H.J. Wenzel, Y. Kinoshita, C.A. Robbins, L.A. Donehower, and P.A. Schwartzkroin. 1996. Loss of the p53 tumor suppressor gene protects neurons from kainate-induced cell death. J. Neurosci. 16, 1337-1345 
  12. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425 
  13. Kim, B.J., C.J. Kim, J. Chun, Y.H. Koh, S.H. Lee, J.W. Hyun, C.Y. Cha, and Y.H. Kook. 2004. Phylogenetic analysis of the genera Streptomyces and Kitasatospora based on partial RNA polymerase $\beta$-subunit gene (rpoB) sequences. Int. J. Syst. Evol. Microbiol. 54, 593-598 
  14. Pitkanen, A. and Kubova, H. 2004. Antiepileptic drugs in neuroprotection. Expert. Opin. Pharmacother. 5, 777-798 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일