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We have conducted in vitro reconstitution study of ferritin from its subunits FerH and FerL. For the reconstitution, FerH was produced from an expression vector construct in Escherichia coli and was purified from a heat treated cell extract by using one-step column chromatography. FerL was expressed as inclusion bodies. The denatured form of FerL was obtained by a simple washing step of the inclusion bodies with 3 M urea. The reconstitution experiment was conducted with various molar ratios of urea-denatured FerH and FerL to make the ferritin nanoparticle with a controlled composition of FerH and FerL. SDS-PAGE analysis of the reconstituted ferritins revealed that the reconstitution required the presence of more than 40 molar$\%$ of FerH in the reconstitution mixture. The assembly of the subunits into the ferritin nanoparticle was confmned by the presence of spherical particles with diameter of 10 nm by the atomic force microscopic image. Further analysis of the particles by using a transmission electron microscope revealed that the reconstituted particles exhibited different percentages of population with dense iron core. The reconstituted ferritin nanoparticles made with molar ratios of [FerH]/[FerL]=l00/0 and 60/40 showed that 80 to $90\%$ of the particles were apoferritin, devoid of iron core. On the contrary, all the particles formed with [FerH]/[FerL]=85/ 15 were found to contain the iron core. This suggests that although FerH can uptake iron, a minor portion of FerL, not exceeding $40\%$ at most, is required to deposit iron inside the particle.

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참고문헌 (19)

  1. Grace, J. E., M. E. Van Eden, and S. D. Aust. 2000. Production of recombinant human apoferritin heteromers. Arch. Biochem. Biophys. 384: 116- 122 
  2. Hempstead, P. D., S. J. Yewdall, A. R. Fernie, D. M. Lawson, P. J. Artymiuk, D. W. Rice, G. C. Ford, and P. M. Harrison. 1997. Comparison of the three-dimensional structures of recombinant human H and horse L ferritins at high resolution. J. Mol. Bol. 268: 424- 448 
  3. Hudson, A. J., S. C. Andrews, C. Hawkins. J. M. Williams, M. Izuhara, F. C. Meldrum, S. Mann, P. M. Harrison. and J. R. Guest. 1993. Overproduction, purification and characterization of the Escherichia coli ferritin. Eur. J. Biochem. 218: 985- 995 
  4. Kim, S. W., Y. H. Kim, and J. W. Lee. 2001. Thermal stability of human ferritin: Concentration dependence and enhanced stability of an N-terminal fusion mutant. Biochem. Biophys. Res. Commun. 289: 125-129 
  5. Meldrum, F. C, B. R. Heywood, and S. Mann. 1992. Magnetoferritin: In vitro synthesis of a novel magnetic protein. Science 257: 522- 523 
  6. Santambrogio, P., S. Levi, A. Cozzi, E. Rovida, A. Albertin, and P. Arosio. 1993. Production and characterization of recombinant heteropolymers of human ferritin H and L chains. J. Biol. Chem. 268: 12744- 12748 
  7. Von Darl, M., P. M. Harrison, and W. Bottke. 1994. Expression in Escherichia coli of a secreted invertebrate ferritin. Eur. J. Biochem. 222: 367- 376 
  8. Levi, S., A. Luzzago, G. Cesareni, A. Cozzi, F. Franceschinelli, A. Albertini, and P. Arosio. 1988. Mechanism of ferritin iron uptake: Activity of the H-chain and deletion mapping of the ferro-oxidase site. J. Biol. Chem. 263: 18086- 18092 
  9. Oh, K. S., Y. S. Park, and H. J. Sung. 2002. Expression and purification of an ACE-inhibitory peptide multimer from synthetic DNA in Escherichia coli. J. Microbiol. Biotechnol. 12: 59- 64 
  10. Lee, J., H. Y. Seo, E. S. Jeon, O. S. Park, K. M. Lee, C. U. Park, and K. S. Kim. 2001. Cooperative activity of subunits of human ferritin heteropolymers in Escherichia coli. J. Biochem. Mol. Biol. 34: 365- 370 
  11. Wong, K. K. W., T. Douglas, S. Gider, D. D. Awschalom, and S. Mann. 1998. Biomimetic synthesis and characterization of magnetic proteins magnetoferritin. Chem. Mater. 10: 279- 285 
  12. Wong, K. K. W., H. Colfen, N. T. Whilton, T. Dougals, and S. Mann. 1999. Synthesis and characterization of hydrophobic ferritin proteins. J. Inorg, Biochem. 76: 187- 195 
  13. Grady. J. K., Z. Zang, T. M. Laue, P. Arosio, and N. D. Chasteen. 2002. Characterization of the H- and L-subunit ratios of ferritins by sodium dodecyl sulfate-capillary gel electrophoresis. Anal. Biochem. 302: 263- 268 
  14. Oh, B. K., Y. K. Kim, Y. M. Bae, W. H. Lee, and J. W. Choi. 2002. Detection of Escherichia coli O157:H7 using imrnunosensor based on surface plasmon resonance. J. Microbiol. Biotechnol. 12: 780- 786 
  15. Levi, S., J. Salfeld, F. Franceschinelli, A. Cozzi, M. H. Dorner, and P. Arosio. 1989. Expression and structural and functional properties of human ferritin L-chain from Escherichia coli. Biochemistry 28: 5179- 5184 
  16. Levi, S., S. J. Yewdall, P. M. Harrison, P. Santambrogio, A. Cozzi, E. Rovida, A. Albertini, and P Arosio, 1992. Evidence that H- and L-chains have co-operative roles in the iron-uptake mechanism of human ferritin. Biochem. J. 288: 591- 596 
  17. Feng, L. and S. Damodaran. 2000. Two dimensional array of magnetoferritin on quarts surface. Thin Solid Films 365: 99-103 
  18. Harrison, P. M. and P. Arosio. 1996. The ferritins: Molecular properties, iron storage function and cellular regulation. Biochim. Biophys. Acta 1275: 161-203 
  19. Rucker, P., F. M. Torti, and S. V. Torti. 1997. Recombinant ferritin: Modulation of subunit stoichiometry in bacterial expression systems. Protein Eng. 10: 967- 973 

이 논문을 인용한 문헌 (7)

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