• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Characterization of the Pediocin Operon of Pediococcus acidilactici K10 and Expression of His-Tagged Recombinant Pediocin PA-1 in Escherichia coli


The relationship between plasmid (~9.5 kb) and pediocin PA-1 in P. acidilactici K10 was confirmed by plasmid curing. The pediocin operon of P. acidilactici K10 was amplified by PCR (polymerase chain reaction), and the nucleotide sequence was analyzed. The sequence of the pediocin operon of P. acidilactici K10 was similar to those of P. acidilactici strains producing pediocin PA-1/ AcH. For the expression of pediocin PA-1 in E. coli, a pQEPED (pQE-30 Xa::mature pedA) was constructed. His-tagged recombinant pediocin PA-1 (-6.5 kDa) was translated by cell-free in vitro transcription and translation using pQEPED as a DNA template. Theresult of slot blotting assay showed that transcription of recombinant pedA in E. coli M15 was induced by the addition of isopropyl-$\beta$-D-thiogalactopyranoside (IPTG) at the final concentration of 1 mM. Although the recombinant pediocin PA-1 inhibited the growth of E. coli, it was expressed in the host strain and purified by nickel-nitrilotriacetic acid (Ni-NTA) metal-affinity chromatography under denaturing condition. This is the first report for the production and one-step purification of biologically active recombinant pediocin PA-1 in E. coli.

저자의 다른 논문

참고문헌 (33)

  1. Klaenhammer, T. R. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12: 39- 86 
  2. Lee, H.-J., C.-S. Park, Y-J. Joo, S.-H. Kim, J.-H. Yoon, Y.-H. Park, I.-K. Hwang, J.-S. Anh, and T-I. Mheen. 1999. Identification and characterization of bacteriocin-producing lactic acid bacteria isolated from kimchi. J. Microbiol. Biotechnol. 9: 282- 291 
  3. Mah, J.-H., K.-S. Kim, J.-H. Park, M.-W. Byun, Y.-B.. Kim, and H.-J. Hwang. 2001. Bacteriocin with a broad antimicrobiol spectrum, produced by Bacillus sp. isolated from kimchi. J. Microbiol. Biotechnol. 11: 577- 584 
  4. Venema, K., J. Kok, J. D. Marugg, M. Y. Toonen, A. M. Ledeboer, G. Venema, and M. L. Chikindas. 1995. Functional analysis of the pediocin operon of Pediococcus acidilactici PAC1.0: PedB is the immunity protein and PedD is the precursor processing enzyme. Mol. Microbiol. 17: 515-522 
  5. Kim, W. J. 1993. Bacteriocins of lactic acid bacteria: Their potentials as food biopreservative. Food Rev. Int. 9: 299-313 
  6. Montville, T. J. and Y. Chen. 1998. Mechanistic action of pediocin and nisin: Recent progress and unresolved questions. Appl. Microbiol. Biotechnol. 50: 511- 519 
  7. Chen, Y., R. Shapira, M. Eisenstein, and T. J. Montville. 1997. Functional characterization of pediocin PA-1 binding to liposomes in the absence of a protein receptor and its relationship to a predicted tertiary structure. Appl. Environ. Microbiol. 63: 524- 531 
  8. Jack, R. W., J. R. Tagg, and B. Ray. 1995. Bacteriocins of Gram-positive bacteria. Microbiol. Rev. 59: 171- 200 
  9. Gibbs, G. M., B. E. Davidson, and A. J. Hiller. 2004. Novel expression system for large-scale production and purification of recombinant class IIa bacteriocins and its application to piscicolin 126. Appl. Environ. Microbiol. 70: 3292- 3297 
  10. Gratia, A. 1925. Sur un remarquable example dantagonisme entre souches de coli-bacille. CR. Soc. Biol. 93: 1040- 1041 
  11. Kwon, D. Y., M. Koo, C. R. Ryoo, C.-H. Kang, K.-H. Min, and W. J. Kim. 2002. Bacteriocin produced by Pediococcus sp, in kimchi and its characteristics. J. Microbiol. Biotechnol. 12: 96- 105 
  12. Motlagh, A., M. Bukhtiyarova, and B. Ray. 1994. Complete nucleotide sequence of pSMB 74, a plasmid encoding the production of pediocin AcH in Pediococcus acidilactici. Lett. Appl. Microbiol. 18: 305- 312 
  13. Tagg, J. R., A. S. Dajani, and L. W. Wannamaker. 1976. Bacteriocins of Gram-positive bacteria. Bacteriol. Rev. 40: 722-756 
  14. Lee, K. H., G. S. Moon. J. Y An, H. J. Lee, H. C. Chang, D. K. Chung, J. H. Lee, and J. H. Kim. 2002. Isolation of a nisin-producing Lactococcus lactis strain from kimchi and characterization of its nisZ gene. J. Microbiol. Biotechnol. 12: 389- 397 
  15. O'Sullivan, D. J. and T. R. Klaenhammer. 1993. Rapid miniprep isolation of high-quality plasmid DNA from Lactococcus and Lactobacillus spp. Appl. Environ. Microbiol. 59: 2730-2733 
  16. Richard, C., D. Drider, K. Elmorjani, D. Marion, and H. Prevost. 2004. Heterologous expression and purification of active divercin V41, a class IIa bacteriocin encoded by a synthetic gene in Escherichia coli. J. Bacteriol. 186: 4276-4284 
  17. Marugg, J. D., C. E Gonzalez, B. S. Kunka, A. M. Ledeboer, M. J. Pucci, M. Y. Toonen, S. A Walker, L. C. Zoetmulder, and P. A. Vandenbergh. 1992. Cloning, expression, and nucleotide sequence of genes involved in production of pediocin PA-1, and bacteriocin from Pediococcus acidilactici PAC1.0. Appl. Environ. Microbiol. 58: 2360- 2367 
  18. Rogers, L. A. 1928. The inhibitory effect of Streptococcus lactis on Lactobacillus bulgaricus. J. Bacteriol. 16: 321-325 
  19. Ray, B. and M. Daeschel, 1992. Food Biopreservatives or Microbiol Origin, pp. 64- 70. CRC Press, Boca Raton, Florida, U.S.A 
  20. Whitehead, H. R. 1933. A substance inhibiting bacterial growth produced by certain strains of lactis streptococci. Biochem. J. 27: 1793- 1800 
  21. Bukhtiyarova, M., R. Yang, and B. Ray. 1994. Analysis of the pediocin AcH gene cluster from plasmid pSMB74 and its expression in a pediocin-negative Pediococcus acidilactici strain. Appl. Environ. Microbiol. 60: 3405- 3408 
  22. Ray, B., R. Schamber, and K. W. Miller. 1999. The pediocin AcH precursor is biologically active. Appl. Environ. Microbiol. 65: 2281- 2286 
  23. Johnsen, L., G. Fimland, V. Eijsink, and J. Nissen-Meyer. 2000. Engineering increased stability in the antiMicrobiol peptide pediocin PA-1. Appl. Environ. Microbiol. 66: 4798-4802 
  24. Makrides, S. C. 1996. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60: 512- 538 
  25. Mattick, A. T. R. and A. Hirsch. 1947. Further observations on an inhibitory substance (nisin) from lactic streptococci. Lancet 2: 5- 7 
  26. Schagger, H. and G. V. Jagow. 1987. Tricine-sodiurn dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368- 379 
  27. Moon, G.-S., W. J. Kim, and M. Kim. 2002. Synergistic effects of .bacteriocin-producing Pediococcus acidilactici K10 and organic acids on inhibiting Escherichia coli O157:H7 and applications in ground beef. J. Microbiol. Biotechnol. 12: 936- 942 
  28. Hurst, A. 1981. Nisin. Adv. Appl. Microbiol. 27: 85- 123 
  29. Moon, G. S., C. H. Chang, Y. R. Pyun, and W. J. Kim. 2004. Isolation, identification, and characterization of a bacteriocinproducing Enterococcus sp. from kimchi and its application to kimchi fermentation. J. Microbiol. Biotechnol. 14: 924-931 
  30. Moon, G. S., J. J. Jeong. G. E. Ji, J. S. Kim, and J. H. Kim. 2000. Characterization of a bacteriocin produced by Enterococcus sp. T7 isolated from humans. J. Microbiol. Biotechnol. 10: 507-513 
  31. Nes. I. F., D. B. Diep, L. S. Havarstein, M. B. Brurberg, V. Eijsink, and H. Holo. 1996. Biosynthesis of bacteriocins in lactic acid bacteria. Antonie van Leeuwenhoek 70: 113- 128 
  32. Gonzalez, C. F. and B. S. Kunka. 1987. Plasmid-associated bacteriocin production and sucrose fermentation in Pediococcus acidilactici. Appl. Environ. Microbiol. 53: 2534- 2538 
  33. Nes, I. F. and H. Holo. 2000. Class II antimicrobiol peptides from lactic acid bacteria. Biopolymers (Peptide Science) 55: 50-61 

이 논문을 인용한 문헌 (5)

  1. 2005. "" Food science and biotechnology, 14(5): 581~585 
  2. 2007. "" Journal of microbiology and biotechnology, 17(4): 663~667 
  3. 2010. "" Journal of food science and nutrition, 15(4): 360~363 
  4. 2010. "" Journal of food science and nutrition, 15(1): 74~77 
  5. 2010. "" Journal of microbiology and biotechnology, 20(8): 1215~1218 


원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일