The objective of this study was to detect and compare the presence of periodontopathogens in the subgingival plaques of gingivitis lesions in adults who wore fixed orthodontic appliances, as opposed to adults who did not wear any orthodontic appliances. Thirty-six individuals participated in this st...
The objective of this study was to detect and compare the presence of periodontopathogens in the subgingival plaques of gingivitis lesions in adults who wore fixed orthodontic appliances, as opposed to adults who did not wear any orthodontic appliances. Thirty-six individuals participated in this study. Ninteen of these subjects did not wear any orthodontic appliances, and these subjects comprised the control group. The other 17 individuals had been wearing fixed orthodontic appliances for at least 3 months each. After a periodontal examination, we collected subgingival plaque samples from the gingivitis lesions of each patient. Using PCR based on 168 rDNA, we detected the presence of 6 putative periodontopathogenic species, Treponema denticola, Porphyromonas gingivalis, Tannerella forsythia (formerly Bacteroides forsythus), Prevotella nigrescens, Prevotella intermedia, and Actinobacillus actinomycetemcomitans. With regard to the presence of individual periodontopathogens, we found that T. forsythia, T. denticola, and P. nigrescens were significantly more common in the samples obtained from the orthodontic patients than in the samples obtained from the non-orthodontic patient controls. Our results indicate that the local changes associated with the wearing of fixed orthodontic appliances may affect the prevalence of periodontopathogens in subgingival dental plaques.
The objective of this study was to detect and compare the presence of periodontopathogens in the subgingival plaques of gingivitis lesions in adults who wore fixed orthodontic appliances, as opposed to adults who did not wear any orthodontic appliances. Thirty-six individuals participated in this study. Ninteen of these subjects did not wear any orthodontic appliances, and these subjects comprised the control group. The other 17 individuals had been wearing fixed orthodontic appliances for at least 3 months each. After a periodontal examination, we collected subgingival plaque samples from the gingivitis lesions of each patient. Using PCR based on 168 rDNA, we detected the presence of 6 putative periodontopathogenic species, Treponema denticola, Porphyromonas gingivalis, Tannerella forsythia (formerly Bacteroides forsythus), Prevotella nigrescens, Prevotella intermedia, and Actinobacillus actinomycetemcomitans. With regard to the presence of individual periodontopathogens, we found that T. forsythia, T. denticola, and P. nigrescens were significantly more common in the samples obtained from the orthodontic patients than in the samples obtained from the non-orthodontic patient controls. Our results indicate that the local changes associated with the wearing of fixed orthodontic appliances may affect the prevalence of periodontopathogens in subgingival dental plaques.
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제안 방법
The 16S rDNA can be used effectively for PCR assays because 16S rDNA is universally found in prokaryotic organisms, and a comparative analysis of 16S rDNA has shown that the variable sequence regions are interspersed with highly conserved regions (Woese, 1987). Therefore, in this study, we performed PCR for the detection of putative periodontal pathogens from the subgingival plaques.
, 1997; Teanpaisan et al, , 1998). These studies were performed on periodontal lesions, endodontic lesions, and healthy periodontium. According to the results of these studies, the prevalence of P.
대상 데이터
A total of 36 individuals participated in this study. Nineteen of these subjects wore no orthodontic appliances, thus comprising the control group.
The other 17 individuals had worn fixed orthodontic appliances for at least 3 months each. All of the participants in the study were recent referrals to the Department of Orthodontics at the Chosun University Dental Hospital, Gwangju, Korea. Our selection criteria were as follows: (a) no al.
PDs were measured at 2 - 9 sites on each tooth (mesiobuccal, buccal, distobuccal, distolingual, lingual, and mesiodistal). One hundred twenty-eight sites from 32 participants were assigned as study sites. The clinical parameters assessed included the following: plaque index (PI) (Silness and Loee, 1964), gingival index (GI) (Loee and Silness, 1963), PD, clinical attachment level (CAL), and bleeding on probing (BOP).
데이터처리
The differences in the prevalence of each bacterial strain were also analyzed using the Chi square test. Differences in BOP, PD, GI, and PI values were analyzed using the Fisher's exact test and trend test. All statistical analyses were carried out with a statistical software package (SPSS for Windows 10.
The differences in the prevalence of each bacterial strain were also analyzed using the Chi square test. Differences in BOP, PD, GI, and PI values were analyzed using the Fisher's exact test and trend test.
성능/효과
These studies were performed on periodontal lesions, endodontic lesions, and healthy periodontium. According to the results of these studies, the prevalence of P. intermedia was elevated in the periodontal lesions, but the prevalence of P. nigrescens was elevated in the endodontic lesions. In this study, we determined the prevalence of P.
nigrescens was elevated in the endodontic lesions. In this study, we determined the prevalence of P. nigrescens in the experimental groups was triple that of P. intermedia, but that the reverse was true in the control group (Table 2). This implies that local environmental changes in the experimental group may have disrupted the normal microorgan- ismic balance inherent to the oral cavities of the orthodontic patients in this study.
denticola was significantly more prevalent in the BOP-positive samples than in the BOP-negative samples from the experimental group. T. denticola, P. gingivalis, T. forsythia, P. intermedia, and A. actinomycetemcomitans were found to be significantly more prevalent in the BOP-positive samples from the control group (p < 0.05) (Table 3). This suggests that a greater variety of periodontopathogens exist in the experimental group, even in the BOP-negative samples.
In the experimental group, which consisted of 17 gingivitis patients with fixed orthodontic appliances, and the control group, which consisted of 19 gingivitis patients without any orthodontic appliances, the prevalence of seven putative periodontopathogens in the subgingival plaque was detected, and is shown in Table 2. T. denticola, T. forsythia, C. rectus, and P. nigrescens were significantly more prevalent in the experimental group than in the controls (p < 0.05) (Table 2). In terms of their prevalence, P.
후속연구
Further inquiries into the specific local environmental changes caused by orthodontic brackets and their effect on the attachment and growth of specific periodontopathogens are clearly warranted. Also, future studies should figs on the effects of these specific periodontopathogens with regard to the progress of periodontal inflammation.
참고문헌 (30)
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