$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

A Putative Early Response of Antifungal Bacillus lentimorbus WJ5 Against the Plant Pathogenic Fungus, Colletotrichum gloeosporioides, Analyzed by a DNA Microarray

The journal of microbiology, v.43 no.3, 2005년, pp.308 - 312  

Lee Young-Keun (Radiation Application Research Division, Korea Atomic Energy Research Institute) ,  Jang Yu-Sin (Radiation Application Research Division, Korea Atomic Energy Research Institute) ,  Chang Hwa-Hyoung (Radiation Application Research Division, Korea Atomic Energy Research Institute) ,  Hyung Seok Won (Radiation Application Research Division, Korea Atomic Energy Research Institute) ,  Chung Hye-Young (Radiation Application Research Division, Korea Atomic Energy Research Institute)

Abstract AI-Helper 아이콘AI-Helper

The global RNA transcription profiles of Bacillus lentimorbus WJ5 under an in vitro co-culture with Colletotrichum gloeosporioides were analyzed in order to study the antagonistic bacteria-fungi interactions. Using a filter membrane system, B. lentimorhus WJ5 was exposed to the spores of C. gloeospo...

주제어

AI 본문요약
AI-Helper 아이콘 AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

  • In this work, custom DNA microarrays of the antago­ nistic bacterium, B. lentimorbus WJ5, were constructed and the RNA transcription profiles from the in vitro co­ culture of B. lentimorbus WJ5 against C. gloeosporioides observed, in order to study the antagonist-fungus interaction.
  • Poor quality spots (sum of median < 300) were filtered from the raw data before analysis. The microarray experiments were repeated twice, and the average of the two gene expres- sion values for each gene used for the analysis. For each hybridized spot, the gene expression value (ratio of the median in the GenePix Pro 3.

대상 데이터

  • The RNA of the B. lentimorbus WJ5 was labeled with cyanine 3-dUTP (Cy3-dUTP, NEN Life Science Products, USA) and sed as the control probe. The RNA of the B.

이론/모형

  • This study was carried out under the Nuclear R&D Pro­ gram of the Ministry of Science and Technology (MOST), Republic of Korea.
본문요약 정보가 도움이 되었나요?

참고문헌 (18)

  1. Bonsall, R.F., D.M. Weller, and L.S. Tomashow. 1999. Quantification of 2,4-diacetylphloroglucinol produced by fluorescent Pseudomonas spp. in vitro and in the rhizosphere of wheat. Appl. Environ. Microbiol. 63, 951-955 

  2. Deepika, M. and G. Anil. 1999. Transcript levels of genes encoding various glycolytic and fermentation enzymes changes in response to abiotic stresses. Plant Sci. 146, 41-51 

  3. Howell, C.R. 1998. The role of antibiosis in biocontrol, p. 173-184. In G.E. Harman and C.P. Kubicek (eds.), Trichoderma and Gliocladium, Vol. 2. Enzymes, biological control and commercial applications, Tayler & Francis, London 

  4. Kunst, F., N. Ogasawara, I. Moszer, A.M. Albertini, G. Alloni, V. Azevedo, M.G. Bertero, P. Bessieres, A. Bolotin, S. Borchert, R. Borriss, L. Boursier, A. Brans, M. Braun, S.C. Brignell, S. Bron, S. Brouillet, C.V. Bruschi, B. Caldwell, V. Capuano, et al. 1997. The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390, 249-256 

  5. Lee, Y.-K., J.-S. Kim, B.I. Jang, Y.-S. Jang, and H.Y. Lee. 2003a. Biological control of Pythium root rot by radiation induced mutant of Bacillus lentimorbus WJ5a17. Korean J. Environ. Biol. 21, 276-285 

  6. Lee, Y.-K., J.-S. Kim, H.-Y. Chung, Y.-S. Jang, and B.I. Jang. 2001a. Two-dimensional electrophoresis analysis of antifungal activity related proteins in Bacillus licheniformis DM3. Korean. J. Environ. Agric. 22, 203-209 

  7. Lee, Y.-K., J.-S. Kim, I.-G. Song, H.-Y. Chung, and H.-H. Chang. 2001b. Characteristics of antifungal bacterium, Bacillus subtilis YS1 and its mutant induced by gamma radiation. Kor. J. Microbiol. 37, 305-311 

  8. Lee, Y.-K., J.-S. Kim, Y.-S. Jang, K.S. Cho, and H.-H. Chang. 2003b. DNA microarray analysis of gene expression in antifungal bacterium of Bacillus lentimorbus WJ5. Kor. J. Microbiol. 39, 141-147 

  9. Leskela, S., E. Wahlstrom, H.-L. Hyyrylainen, M. Jacobs, A. Palva, M. Sarvas, and V.P. Kontinen. 1999. Ecs, an ABC transporter of Bacillus subtilis: dual signal transduction functions affecting expression of secreted proteins, as well as their secretion. Mol. Microbiol. 31, 533-543 

  10. Leverrier, P., J.P. Vissers, A. Rouault, P. Boyaval, and G. Jan. 2004. Mass spectrometry proteomic analysis of stress adaptation reveals both common and distinct response pathways in Propionibacterium freudenreichii. Arch. Microbiol. 181, 215-230 

  11. Meyer, V. and U. Stahl. 2003. The influence of co-cultivation on expression of the antifungal protein in Aspergillus giganteus. J. Basic Microbiol. 43, 68-74 

  12. Mondal, S.N. and M. Hyakumachi. 2000. Soil factors affecting carbon loss and pathogenicity of oospores of Pythium aphanidermatum. Soil Biol. Biochem. 32, 111-118 

  13. Raaijmakers, J.M., M. Vlami, and J.T. de Souza. 2002. Antibiotic production by bacterial biocontrol agents. Antonie Van Leeuwenhoek 81, 537-547 

  14. Steijl, H., G.J. Niemann, and J.J. Boon. 1999. Changes in chemical composition related to fungal infection and induced resistance in carnation and radish investigated by pyrolysis mass spectrometry. Physiol. Mol. Plant Pathol. 55, 297-311 

  15. Trowsdale, J., S.M.H. Chen, and J.A. Hoch. 1978. Genetic analysis of phenotype revertants of spo0A mutants in Bacillus subtilis: a new cluster of ribosomal genes, p. 131-135. In G. Chambliss and J.C. Vary (eds.), Spores VII. American Society for Microbiology, Washington, D.C 

  16. Whipps, J.M. 1997. Developments in the biological control of soilborne plant pathogens. Adv. Bot. Res. 26, 1-134 

  17. Whipps, J.M. 2001. Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot. 52, 487-511 

  18. Ye, R.W., T. Wang, L. Bedzyk, and K.M. Croker. 2001. Applications of DNA microarrays in microbial systems. J. Microbiol. Meth. 47, 257-272 

저자의 다른 논문 :

관련 콘텐츠

섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트