[논문]Doenjang Extract Has Anticancer Activity and Induces Apoptosis in AGS Human Gastric Adenocarcinoma

Hwang, Kyung-Mi; Lee, Jeong-Min; Park, Kun-Young

doi:10.3746/jfn.2005.10.2.167

Doenjang Extract Has Anticancer Activity and Induces Apoptosis in AGS Human Gastric Adenocarcinoma 원문보기

Journal of food science and nutrition, v.10 no.2, 2005년, pp.167 - 171

Hwang, Kyung-Mi (Department of Food Science and Nutrition, Pusan National University) , Lee, Jeong-Min (Department of Food and Life Sciences, Nambu University) , Park, Kun-Young (Department of Food Science and Nutrition, Pusan National University)

Abstract ▼ AI-Helper

The anticancer and apoptotic effect of chloroform extract from 24 month-fermented doenjang were investigated in AGS human gastric adenocarcinoma cells. The chloroform extract of 24 month-fermented doenjang inhibited the AGS gastric cancer cell growth in a dose-dependent manner. It has been confirmed by observing the cell distribution under inverted microscope. Approximately, 48 hour treatment of $100\;{\mu}g/mL$ doenjang extract inhibited AGS cancer cell growth by $76.7\%$, respectively. The growth inhibition may be caused by apoptosis of AGS cancer cells after 48 hour treatment of 24 month-fermented doenjang extract. It has been demonstrated by cell cycle arrest that revealed the shift from $G_2+M\;to\;G_0+G_1$ phase and the formation of apoptotic bodies. The fermentation period playa critical role in cell cycle arrest, in which 24 month-fermented doenjang extract was more effective than 12 month-fermented doenjang extract. The treatment of 24 month-fermented doenjang extract for 48 hours has induced intercellular Bax and decreased Bcl-2 level, indicating that it may regulate the expression level of Bax/Bcl-2 proteins. Thus, 24 month-fermented doenjang extract seems to have anticancer effect via cancer cell growth inhibition induced by apoptosis process.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

DNA flow cytometric cell cycle analysis was performed comparing untreated controls with cells treated with non-fermented (non-ripen) doenjang extract. After treatment with doenjang extract for 48 hour, cells were collected and stained with PI, and then flow cytometric cell cycle analysis was performed.
Two year fermented doenjang showed higher antimutagenic, antitumor and antimetastatic activities compared to 3, 6 and 12 month fermented doenjangs. Thus, this study has mostly focused on growth inhibition of AGS cancer cells by treatment of doenjang extract fermented for 24 months. The chloroform extract of 24 month-fermented doenjang has significantly inhibited the growth rate of AGS cancer cells in a dosedependent manner (Fig.

대상 데이터

The AGS human gastric adenocarcinoma cell line (ATCC CRL-1739) wa옹 purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). The cells were cultured in DMEM (Whitaker, USA) supplemented with 10% heat-inactivated (55℃, 30 min) fetal bovine serum (FBS, Whitaker, USA), 2 mM L-glutamine (Sigma, USA), 100 U/mL penicillin and 100 mg/mL strptomycin (BioWhitaker, USA) at 37℃ in an atmosphere of 5% CO?.

데이터처리

For growth inhibition test, statistically significant differences between groups were determined with Student's Z-test. In all analyses, a value of p <0,05 was considered statistically significant.

성능/효과

In conclusion, the chloroform extract of 24 month-fermented doenjang inhibited the AGS gastric cancer cell growth in dose-dependent manner. The growth inhibition may be caused by apoptosis of AGS cancer cells after 48 hour treatment of 24 month-fermented doenjang extract.
These phenomena were induced via several physiological or pathological mediators such as oxidative stress, expression of ligands and receptors (TRAIL, TNF, Fas, FasL), activation of caspase pathway, and reduction of antiapoptotic proteins (Bcl-2, Bcl-xL, p35). In this study, 24 month-fermented doenjang extract shifted cell cycle from G2 + M to G0 + G1 phase, indicating that cell division or mitosis was blocked in AGS cancer cells (Fig. 3). Interestingly, 12 month-fermented doenjang extract did not show any shift of cell cycle, compared to control, supporting that fermentation period in manufacturing doenjang may play a critical role in anticancer activity.

참고문헌 (14)

Park KY, Lee KB. 1987. Degradation of aflatoxin during manufacturing of doenjang and kanjang by traditional method. J Colle Home Eco 13: 49-56
Park KY, Lee KB, Bullerman LB. 1988. Aflatoxin production by Aspergillus parasiticus and its stability during the manufacture of Korean soypaste (doenjang) and soysauce (kanjang) by traditional method. J Food Protect 51: 938-945

상세보기
Park KY, Lee ES, Moon SH, Cheigh HS. 1989. Effects of browning products and charcoal on the degradation of aflatoxin B1 in Korean soysauce (kanjang) and its model system. Korean J Food Sci Technol 21: 419-424

원문보기 상세보기
Jing Y, Waxman S. 1995. Structural requirements for differentiation-induction and growth-inhibition of mouse erythroleukemia cells by isoflavones. Anticancer Res 15: 1147-1152

상세보기
Rao AV, Sung MK. 1995. Saponins as anticarcinogens. J Nutr 125: 717s-724s
Yavelow J, Finlay TH, Kennedy AR, Troll W. 1983. Bowman-Birk soybean protease inhibitor as an anticarcinogen. Cancer Res 43: 2454s-2459s
Kurech T, Kikugawa K, Fukuda S, Hasunuma M. 1985. Inhibition of N-nitrosamine formation by soya products. Food Cosmet Toxicol 19: 425-428

상세보기
Park KY, Jung KO, Rhee SH, Choi YH. 2003. Antimutagenic effects of doenjang (Korean fermented soypaste) and its active compounds. Mut Res 523: 43-53
Kim MK, Moon SH, Choi JW, Park KY. 1999. The effect of doenjang (Korean soypaste) on the liver enzyme activities of the sarcoma-180 cell transplanted mice. J Food Sci Nutr 4: 260-264
Chandra J, Samali A, Orrenius S. 2000. Triggering and modulation of apoptosis by oxidative stress. Free Rad Biol Med 29: 323-333

상세보기
Green DR. 2000. Apoptotic pathways: paper wraps stone blunts scissors. Cell 102: 1-4

상세보기
Zou H, Li X, Wang X. 1999. An APAF-1 cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chern 274: 11549-11556

상세보기
Saleh A, Srinivasula SM, Acharya S, Fishel R, Alnemri ES. 1999. Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation. J Biol Chem 274: 17941-17945

상세보기
Paul-Samojedny M, Kokocinska D, Samojedny A, Mazurek U, Partyka R, Lorenz Z, Wilczak T. 2005. Expression of cell survival/death genes: Bcl-2 and Bax at the rate of colon cancer prognosis. Biochem Biophys Acta 1741: 25-29

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증