[논문]Determination of Catechin Compounds in Korean Green Tea Infusions under Various Extraction Conditions by High Performance Liquid Chromatography

Cheong, Won-Jo; Park, Moon-Hee; Kang, Gyoung-Won; Ko, Joung-Ho; Seo, You-Jin

doi:10.5012/bkcs.2005.26.5.747

Determination of Catechin Compounds in Korean Green Tea Infusions under Various Extraction Conditions by High Performance Liquid Chromatography 원문보기

Bulletin of the Korean chemical society, v.26 no.5, 2005년, pp.747 - 754

Cheong, Won-Jo (Department of Chemistry and Institute of Basic Research, Inha University) , Park, Moon-Hee (Department of Chemistry and Institute of Basic Research, Inha University) , Kang, Gyoung-Won (Department of Chemistry and Institute of Basic Research, Inha University) , Ko, Joung-Ho (Department of Chemistry and Institute of Basic Research, Inha University) , Seo, You-Jin (Department of Chemistry and Institute of Basic Research, Inha University)

Abstract ▼ AI-Helper

Liquid chromatographic methods with UV and fluorescence detection have been used to determine the levels of (+)-catechin, (-)-epicatechin, (-)-epicatechin gallate, and (-)-epigallocatechin gallate in Korean green tea infusions. The extracts of Korean green tea leaves or powders in water at various temperatures (100 ${^{\circ}C}$, 80 ${^{\circ}C}$, 60 ${^{\circ}C}$) and time, were washed with chloroform and re-extracted to ethyl acetate. The ethyl acetate phase was dried and re-dissolved in methanol and analyzed. Five catechin compounds were separated by gradient elution. The flavonoids were found decomposed on prolonged extraction, thus exhaustive extraction by a Soxhlet apparatus was found useless for green tea. Some unknown components were found in the extracts at 100 ${^{\circ}C}$. When the green tea was filtered and re-extracted with new fresh water, still some flavonoids were extracted. However, the contents of flavonoids in the third extract were found negligible. The flavonoid extraction rate of green tea powders was higher than that of green tea leaves, but flavonoid decomposition of green tea powders was also faster than that of green tea leaves. The traditional way of drinking green tea was found appropriate in view of flavonoids intake.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

The purpose of this study is to determine types and amounts of flavonoids in Korean green tea by both UV and fluorescence detection, and to examine the extraction efficiency of flavonoids by hot water with respect to extraction time and temperature.

가설 설정

The calibration curves of standard flavonoids are given in Figure 4. Good linear relationships were observed for all the flavonoids. The variations of concentrations of epicatechin, epicatechin gallate, epigallocatechin gallate in the extracts of green tea leaves with respect to extraction time and temperature are shown in Figure 5.
Figure 6. The Effects of time on extraction of unknown compounds.

제안 방법

In this study, epicatechin, epicatechin gallate, epigallocatechin gallate were determined by UV detection at 280 nm, and catechin and epicatechin, by fluorescence detection (excitation 235 nm, emission 315 nm). Epigallocatechin was, however, unable to determine by either method.
The concentration range of each flavonoid in the green tea extracts was roughly measured in the preliminary experiments, and the concentrations of individual components were determined based on the chromatographic data of a standard mixture. The calibration curves (peak area count vs.
The purpose of Soxhlet extraction was to determine the total amount of each flavonoid in the green tea. As described earlier, however, the flavonoids decomposed on prolonged extraction, thus the concentrations of Soxhlet extraction were even lower than those of the second extraction.

대상 데이터

A chromatographic system composed of two Shimadzu (Tokyo, Japan) 10AD pumps, a Rheodyne (Cotati, CA, USA) 7520 injector with a 0.5 µL internal sample loop, a Samsung (Suwon, Korea) SLC-200 UV/VIS detector, and a Shimadzu DGU-14A degasser was used. An Alltima C18 microcolumn (0.
The structure of flavonoids is based on a C6-C3-C6 skeletal where two phenyl rings are connected via a unit composed of three carbons. Two or more hydroxyl groups are attached to each phenyl ring, thus flavonoids are classified as polyphenolic compounds.

참고문헌 (39)

Smith, H. Flavonoids in Phytochrome; Mitrakos, K, Shropshire, W., Eds.; Academic Press: New York and London, 1972; p 433
Robards, K.; Antolovich, M. Analyst 1997, 122, 11R

상세보기
Markham, K. R. Techniques of Flavonoid Identification; Academic Press: New York and London, 1981
Singleton, V. L.; Esau, P. Phenolic Substances in Grapes and Wine and Their Significance; Acaedmic Press: New York, 1969
Afanas'ev, I. B.; Dorozhko, A. I.; Brodskii, A. V.; Kostyuk, A.; Potapovitch, A. J. Biochem. Pharmacol. 1989, 38, 1763

상세보기
Mora, A.; Paya, M.; Rios, J. L.; Alcaraz, M. J. Biochem. Pharmacol. 1990, 40, 793

상세보기
Rice-Evans, C. A.; Miller, N. J.; Pnganga, G. Free Radical Biology and Medicine 1993, 20, 933
Siemann, E. H.; Creasy, L. L. Amer. J. Enol. Vitic. 1992, 43, 49
Jang, M.; Cai, L.; Udeani, G. O.; Slowing, K. V.; Thomas, C. F.; Beecher, C. W. W.; Fong, H. H. S.; Farnsworth, M. R.; Kinghorn, A. D.; Mehta, R. G.; Moon, R. C.; Pezzuto, J. M. Science 1997, 275, 218

상세보기
Middleton, E. Int. J. Pharmacog. 1996, 34, 344

상세보기
Steinmetz, K. A.; Porter, J. D. Cancer Causes Control 1991, 2, 427

상세보기
Law, M. R.; Morris, J. K. Eur. J. Clin. Nutr. 1998, 52, 549

상세보기
Tijburg, L. B. M.; Mattern, T.; Folts, J. D.; Weisgerber, U. M.; Katan, M. B. Crit. Rev. Food Sci. Nutr. 1997, 37, 771

상세보기
Rimm, E. B.; Katan, M. B.; Ascherio, A.; Stanpfer, M. J.; Willett, W. C. Ann. Intern. Med. 1996, 125, 384

상세보기
Ferry, D. R.; Smith, A.; Malkhandi, J.; Fyfe, D. W.; De Takat, P. G.; Anderson, D.; Baker, J.; Kerr, D. J. Clin. Cancer Res. 1996, 2, 659

상세보기
Lotito, S. B.; Fraga, C. G. Free Radical Biology and Medicine 1998, 24, 435

상세보기
Morgan, J. F.; Klucas, R. V.; Grayer, R. J.; Abian, J.; Becana, M. Free Radical Biology and Medicine 1997, 22, 861

상세보기
Ranabahu, P.; Harborne, J. B. Biochemical Systematics and Ecology 1993, 21, 715

상세보기
Choi, B. H. Korean J. Plant Taxonomy 1994, 24, 1
Viguera, G. C.; Barberan, T. F.; Ferreres, F.; Artes, F.; Lorentes, T. F. Z. Lebensm. Unters. Forsch. 1993, 197, 255

상세보기
Mouly, P. P.; Gaydou, E. M.; Estienne, J. M. J. Chromatogr. 1993, 634, 1529
Bronner, W. E.; Beecher, G. R. J. Chromatogr. A 1995, 705, 247

상세보기
Mouly, P. P.; Gaydou, E. M.; Faure, R.; Estinne, J. M. J. Agric. Food Chem. 1997, 45, 373

상세보기
Rodriguez, M. A.; Mnalovana, S.; Perez, J. P.; Borges, T.; Gracia- Montelongo, F. J. J. Chromatogr. A 2001, 912, 249

상세보기
Peinado, J.; Florindo, J. Analyst 1988, 113, 555

상세보기
Kartnig, T.; Gobel, I. J. Chromatogr. A 1996, 740, 99

상세보기
Schmidt, T. J.; Merfort, I. J. Chromatogr. 1993, 634, 350

상세보기
Schmidt, T. J.; Merfort, I.; Willuhn, G. J. Chromatogr. A 1994, 669, 236

상세보기
Galletti, G. C.; Reeves, J. B. Organic Mass Spectometry 1992, 27, 226

상세보기
Stecher, G.; Huck, C. W.; Popp, M.; Bonn, G. K. Fresenius J. Anal. Chem. 2001, 371, 73

상세보기
Tolonen, A.; Uusitalo, J. Rapid Comm. Mass Spectrom. 2004, 18, 3113

상세보기
He, X. G. J. Chromatogr. A 2000, 880, 203

상세보기
Lee, M.-J.; Prabhu, S.; Meng, X.; Li, C.; Yang, C. S. Anal. Biochem. 2000, 279, 164

상세보기
Wright, L. P.; Aucamp, J. P.; Apostolides, Z. J. Chromatogr. A 2001, 919, 205

상세보기
Lee, B.-L.; Ong, C.-N. J. Chromatogr. A 2000, 881, 439

상세보기
Sano, M.; Tabata, M.; Suzuki, M.; Degawa, M.; Miyase, T.; Maeda-Yamamoto, M. Analyst 2001, 126, 816

상세보기
Tsao, R.; Deng, Z. Y. J. Chromatogr. B 2004, 812, 85

상세보기
Zuo, Y. G.; Chen, H.; Deng, Y. W. Talanta 2002, 57, 307

상세보기
Fernandez, P. L.; Pablos, F.; Martin, M.; Gonzalez, A. G. J. Agric. Food Chem. 2002, 50, 1833

상세보기

저자의 다른 논문 :

원문 URL 링크

DOI : 10.5012/bkcs.2005.26.5.747 [무료]
대한화학회 : 저널> 권호
Korea Open Access Journals : 저널
AccessON : 저널

*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.

오픈액세스(OA) 유형

GOLD

오픈액세스 학술지에 출판된 논문

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관

저장형식

Text(ASCII format)
Excel format
RefWorks Direct Export
RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

연합인증