[논문]Determination of Total Phenols in Environmental Waters by Capillary-HPLC with U.S.E.P.A. Classified Eleven Priority Pollutant Phenols after Nitrosation and Their Visible Spectrophotometric Detection

Chung, Yong-Soon

doi:10.5012/bkcs.2005.26.2.297

Determination of Total Phenols in Environmental Waters by Capillary-HPLC with U.S.E.P.A. Classified Eleven Priority Pollutant Phenols after Nitrosation and Their Visible Spectrophotometric Detection 원문보기

Bulletin of the Korean chemical society, v.26 no.2, 2005년, pp.297 - 302

Chung, Yong-Soon (Department of Chemistry, Chungbuk National University)

Abstract ▼ AI-Helper

The determination of total phenols was accomplished by capillary-high performance liquid chromatography (capillary-HPLC) after nitrosation of the U.S.E.P.A. classified 11 priority pollutant phenols, using the nitrosated parent phenol (POHNO) as a reference for calibration. The optimum mobile phase composition for this analysis was found by examining the effect of changing the percentage of acetonitrile (MeCN) in the mobile phase on retention factors (k values) and peak intensities. As MeCN percentage was increased, k values were reduced and peak intensities were generally increased. From the results obtained, it was found that the optimum mobile phase was 90%(v/v) MeCN solution at pH 8.0, the detection wavelength of 400 nm, and a detection limit (D.L., concentration at signal to noise ratio (S/N) of 3.0) of 4.5 ${\times}$ $10^{-7}$ M. In addition, 10 of the 11 phenols present in mineral or waste water were separated after the nitrosation by capillary-HPLC. The optimum mobile phase for separation was a 40%(v/v) MeCN solution at pH 5.0.

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문제 정의

This work describes a sensitive capillary-HPLC method for the continuous determination of phenols in environmental mineral and waste-water. The major advantage of capillary-HPLC was consumption of a small quantity of mobile phase (flow rate: 4.

제안 방법

Mineral water from the Chojeong area near Cheongju-city and waste water from the Cheongju Industrial Environmental Facilities Management Corporation spiked with the 11 priority pollutant phenols, nitrosated by the same method described in the preparation of the standard calibration reference solution, were used as sample solutions in this study. The sample solutions were prepared as follows; 0.
The objective of this work was to find optimum capillary-HPLC mobile phase composition for the determination of total phenols in a nitrosated phenol solution, in which the retention times of most nitrosated and non-nitrosated phenols in capillary-HPLC are same.
0% relative error. The tolerance of the developed method to these chemical species was studied using the mineral and waste water spiked with the POHNO solution in this study. As shown in Table 2, aniline, fomaldehyde, and most of the ions tested did not interfere with the determination of total phenols.

성능/효과

The optimum mobile phase for the determination of the total phenols was found by examining the effect of changing the percentage of MeCN in the mobile phase on k values and peak intensities of the nitrosated 11 phenols. As MeCN percentage was increased, k values were decreased and peak intensities were generally increased. The optimum mobile phase was found to be 90%(v/v) MeCN at pH 8.
The results are shown in Figure 6 and Table 3. It was apparent that the percentage recovery of total phenols in spiked mineral and waste-water samples was sufficiently accurate. The standard deviation of the recovery was 2.

참고문헌 (18)

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DOI : 10.5012/bkcs.2005.26.2.297 [무료]
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