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The Schizosaccharomyces pombe Gene Encoding ${gamma}-Glutamyl$ Transpeptidase I Is Regulated by Non-fermentable Carbon Sources and Nitrogen Starvation

The journal of microbiology, v.43 no.1, 2005년, pp.44 - 48  

Kim, Hong-Gyun (Division of Life Sciences, Kangwon National University) ,  Park, Hey-Jung (Division of Life Sciences, Kangwon National University) ,  Kang, Hyun-Jung (Division of Life Sciences, Kangwon National University) ,  Lim, Hye-Won (Division of Life Sciences, Kangwon National University) ,  Kim, Kyung-Hoon (Division of Life Sciences, Kangwon National University) ,  Park, Eun-Hee (College of Pharmacy, Sookmyung Women's University) ,  Ahn, Ki-Sup (Department of Health and Environment, Baekseok College) ,  Lim, Chang-Jin (Division of Life Sciences, Kangwon National University)

Abstract AI-Helper 아이콘AI-Helper

In our previous study, the first structural gene (GGTI) encoding ${\gamma}-glutamyl$ transpeptidase was cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its transcription, using the GGTI-lacZ fusion gene, containing the 1,085 bp upstream region from the trans...

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제안 방법

  • To identify the upstream region responsible for the induction of the GGTI gene by non-fermentable carbon sources and nitrogen starvation, the upstream region was serially deleted from the original fusion plasmid pGT98 by PCR (Fig. 4). Appropriately synthesized primers were used for the PCR amplification by Ex Taq DNA poly­ merase (TaKaRa, Shuzo Co.

이론/모형

  • The yeast cells were grown at 30℃, with shaking, and the cell growth was monitored by the absorbance at 600 nm. The P-galactosidase activity in the extracts was measured by a spectrophotometric method, using o-nitrophenyl P-D- galactopyranoside (ONPG) as a substrate (Guarente, 1983). The protein content of the extracts was measured by the Bradford method (Bradford, 1976), using bovine serum albumin as a standard.
  • The P-galactosidase activity in the extracts was measured by a spectrophotometric method, using o-nitrophenyl P-D- galactopyranoside (ONPG) as a substrate (Guarente, 1983). The protein content of the extracts was measured by the Bradford method (Bradford, 1976), using bovine serum albumin as a standard.
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참고문헌 (22)

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  6. Hiesinger, M., S. Roth, E. Meissner, and H.-J. Schuller. 2001. Contribution of Cat8 and Sip4 to the transcriptional activation of yeast gluconeogenic genes by carbon source-responsive elements. Curr. Genet. 39, 68-76 

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  9. Lee, Y.-Y., S.-J. Kim, E.-H. Park, and C.-J. Lim. 2003. Glutathione content and the activities of glutathione synthesizing enzymes in fission yeast are modulated by oxidative stress. J. Microbiol. 41, 248-251 

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  14. Nakajima, T., S. Ohta, N. Murayama, and A. Sato. 1994. Carbohydrate- related regulation of the ethanol-induced increase in serum $\gamma$ -glutamyl transpeptidase activity in adult men. Am. J. Clin. Nutr. 60, 87-92 

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  16. Park, H.-J., H.-W. Lim, K. Kim, I.-H. Kim, E.-H. Park, and C.-J. Lim. 2004. Characterization and regulation of the $\gamma$ -glutamyl transpeptidase gene from the fission yeast Schizosaccharomyces pombe. Can. J. Microbiol. 50, 61-66 

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  19. Springael, J.-Y. and M.J. Penninckx. 2003. Nitrogen-source regulation of yeast $\gamma$ -glutamyl transpeptidase synthesis involves the regulatory network including the GATA zinc-finger factor Gln3, Nil1/Gat1 and Gzf3. Biochem. J. 371, 589-595 

  20. Kim, S.-J., H.-G. Kim, B.-C. Kim, K. Kim, E.-H. Park, and C.-J. Lim. 2004. Transcriptional regulation of the Gene encoding $\gamma$ -glutamylcysteine synthetase from the fission yeast Schizosaccharomyces pombe. J. Microbiol. 42, 233-238 

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