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논문 상세정보


TRPM7, a cation channel protein permeable to various metal ions such as $Mg^{2+}$, is ubiquitously expressed in variety of cells including lymphocytes. The activity of TRPM7 is tightly regulated by intracellular $Mg^{2+}$, thus named $Mg^{2+}$-inhibited cation (MIC) current, and its expression is known to be critical for the viability and proliferation of B lymphocytes. In this study, the level of MIC current was compared between immature (WEHI-231) and mature (Bal-17) B lymphocytes. In both cell types, an intracellular dialysis with $Mg^{2+}$-free solution (140 mM CsCl) induced an outwardly-rectifying MIC current. The peak amplitude of MIC current and the permeability to divalent cation ($Mn^{2+}$) were several fold higher in Bal-17 than WEHI-231. Also, the level of mRNAs for TRPM7, a molecular correspondence of the MIC channel, was significantly higher in Bal-17 cells. The amplitude of MIC was further increased, and the relation between current and voltage became linear under divalent cation-free conditions, demonstrating typical properties of the TRPM7. The stimulation of B cell receptors (BCR) by ligation with antibodies did not change the amplitude of MIC current. Also, increase of extracellular $[Mg^{2+}]_c$ to enhance the $Mg^{2+}$ influx did not affect the BCR ligation-induced death of WEHI-231 cells. Although the level of TRPM7 was not directly related with the cell death of immature B cells, the remarkable difference of TRPM7 might indicate a fundamental change in the permeability to divalent cations during the development of B cells.

참고문헌 (23)

  1. Igarashi H, Kuwahara K, Nomura J, Matsuda A, Kikuchi K, Inui S, Sakaguchi N. B cell Ag receptor mediates different types of signals in the protein kinase activity between immature B cell and mature B cell. J Immunol 153: 2381-2389, 1994 
  2. Nadler MJ, Hermosura MC, Inabe K, Poerraud AL, Zhu Q, Stokes AJ, Kurosaki T, Kinet J Ph, Penner R, Scharenberg AM, Fleig A. LTRPC7 is a MgATP-regulated divalent cation channel required for cell viability. Nature 411: 590-595, 2001 
  3. Nam JH, Yun SS, Kim TJ, Uhm D-Y, Kim SJ. Slow and persistent increase of $[Ca^{2+}]_c$ in response to ligation of surface IgM in WEHI-231 cells. FEBS Lett 535: 113-118, 2003 
  4. Prakriya M, Lewis RS. Separation and characterization of currents through store-operated CRAC channels and $Mg^{2+}-inhibited$ cation (MIC) channels. J Gen Physiol 119: 487-507, 2002 
  5. Schlingmann KP, Weber S, Peters M, Niemann-Nejsum L, Vitzthum H, Klingel K, Kratz M, Haddad E, Ristoff E, Dinour D, Syrrou M, Nielsen S, Sassen M, Waldegger S, Seyberth HW, Konrad M. Hypomagnesemia with secondary hypocalcemia is caused by mutations in TRPM6, a new member of the TRPM gene family. Nat Genet 31: 166-170, 2002 
  6. Tasker L, Marshall-Clarke S. Antigen receptor signaling in apoptosis-resistant mutants of WEHI-231 cells. Immunology 99: 385-393, 2000 
  7. Nam JH, Woo J-E, Uhm D-Y, Kim SJ. Membrane-delimited regulation of background K+ channels by MgATP in murine immature B lymphocytes. J Biol Chem 279: 20643-20654, 2004 
  8. Runnels LW, Yue L, Clapham DE. The TRPM7 channel is inactivated by $PIP_2$ hydrolysis. Nat Cell Biol 4: 329-336 
  9. Saito K, Tolias KF, Saci A, Koon HB, Humphries LA, Scharenberg A, Rawlings DJ, Kinet J-P, Carpenter CL. BTK regulates PtdIns-4,5-P2 synthesis: importance for calcium signaling and PI3K activity. Immunity 19: 669-678, 2003 
  10. Monteilh-Zoller M, Hermosura MC, Nadler MJS, Scharenberg AM, Penner R, Fleig A. TRPM7 provides an ion channel mechanism for cellular entry of trace metal ions. J Gen Physiol 12: 49-60, 2003 
  11. Chubanov V, Waldegger S, Schnituzler MM, Vitzthum H, Sassen MC, Seyberth HW, Konrad M, Gudermann T. Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia. Proc Nat Acad Sci 101: 2894-2899, 2004 
  12. Jiang X, Newell EW, Schlichter LC. Regulation of a TRPM7-like current in rat brain microglia. J Biol Chem 278: 42867-42876, 2003 
  13. Schmitz C, Perraud A-L, Johnson CO, Inabe K, Smith MK, Penner R, Kurosaki T, Fleig A, Scharenberg AM. Regulation of vertebrate cellular $Mg^{2+}$ homeostasis by TRPM7. Cell 114: 191-200, 2003 
  14. Hermosura MC, Monteilh-Zoller MK, Scharenberg AM, Penner R, Fleig A. Dissociatin of the store-operated calcium current ICRAC and the Mg-nucleotide-regulated metal ion current MagNuM. J Physiol (Lond) 539: 445-458, 2002 
  15. Merritt JE, Jacob R, Hallam TJ. Use of manganese to discriminate between calcium influx and mobilization from internal stores in stimulated human neutrophils. J Biol Chem 264: 1522-1527, 1989 
  16. Runnels LW, Yue L, Clapham DE. TRP-PLIK, a bifunctional protein with kinase and ion channel activities. Science 291: 1043-1047, 2001 
  17. Bakowski D, Parekh AB. Permeation through store-operated CRAC channels in divalent-free solution: potential problems and implications for putative CRAC channel genes. Cell Cal 32: 379-391, 2002 
  18. Perraud AL, Knowles HM, Schmitz C. Novel aspects of signaling and ion-homeostasis regulation in immunocytes. The TRPM ion channels and their potential role in modulating the immune response. Mol Immunol 41: 657-673, 2004 
  19. Wu Y, Pani G, Siminovitch KA, Hozumi N. Antigen receptortriggered apoptosis in immature B cell lines is associated with the binding of a 44-kDa phosphoprotein to the PTP1C tyrosine phosphatase. Eur J Immunol 25: 2279-2284, 1995 
  20. Dorovkov MV, Ryazanov AG. Phosphorylation of annexin by TRPM7 channel-kinase. J Biol Chem 279: 50643-50646, 2004 
  21. Montell C. $Mg^{2+}$ homeostasis: the $Mg^{2+}$nificent TRPM chanzymes. Curr Biol 13: R799-R801, 2003 
  22. Kozak JA, Cahalan M. MIC channels are inhibited by internal divalent cations but not ATP. Biophys J 84: 922-927, 2003 
  23. Clapham DE. TRP channels as cellular sensors. Nature 426: 517-524, 2003 

이 논문을 인용한 문헌 (1)

  1. 2006. "" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 10(1): 19~24 


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