[논문]Free Radical Scavenging and Lipid Peroxidation Inhibition Potential of Hygrophila auriculata

Vijayakumar, Madhavan; Govindarajan, Raghavan; Shirwaikar, Arun; Kumar, Vivek; Rawat, Ajay Kumar Singh; Mehrotra, Shanta; Pushpangadan, Palpu

Free Radical Scavenging and Lipid Peroxidation Inhibition Potential of Hygrophila auriculata 원문보기

Natural product sciences, v.11 no.1, 2005년, pp.22 - 26

Vijayakumar, Madhavan (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) , Govindarajan, Raghavan (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) , Shirwaikar, Arun (College of Pharmaceutical sciences, MAHE) , Kumar, Vivek (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) , Rawat, Ajay Kumar Singh (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) , Mehrotra, Shanta (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) , Pushpangadan, Palpu (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute)

Abstract ▼ AI-Helper

Hygrophila auriculata (K. Schum.) Heine is a wild herb commonly found in moist places on the banks of tanks, ditches and paddy fields throughout India and is one of the main sources of Ayurvedic 'Rasayana' drug. The free radical scavenging potential of 50% aqueous alcoholic extract of H. auriculata (HAEt) was studied for DPPH scavenging activity, nitric oxide, hydroxyl radical and ferryl bipyridyl complex scavenging activity along with lipid peroxidation and total antioxidant capacity inhibition using Thiobarbituric acid reactive substances (TBARS) in rat liver homogenate. Integral antioxidative capacity was determined by photochemiluminescence assay. HAEt showed good radical scavenging activity at various concentrations $(200-1000\;{\mu}g/ml)$ against 1,1 diphenyl, 2-picrylhydrazyl (DPPH) (32.32-77.02%) with moderate scavenging activity against Nitric oxide (12.46-52.84%), hydroxyl radical (11.69-55.26%), ferryl bipyridyl complex (17.66-58.67%) and lipid peroxidation (0.829-0.416nmoles/mg protein). The above results indicate HAEt to be very effective antioxidant. It was suggested that the varied therapeutic activities claimed for the plant in the Indian indigenous systems of medicine may be in the part due to the free radical scavenging and/ or potent antioxidant activity.

주제어

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문제 정의

However to date no antioxidant investigations have been reported in this plant. Therefore, the present study was undertaken to investigate the free radical scavenging potential of the hydro alcoholic extract of H. auriculata (HAEt).

제안 방법

Research Institute, Lucknow, India. These were kept in the departmental animal house at 26±2℃ and relative humidity 44-55% light and dark cycles of 10 and 14 hr respectively for one week before the experiment. Animals were provided with rodent diet (Amruth, India) and water ad libitum.

대상 데이터

HAEt was added to obtain final concentrations ranging from 50-400 μg/ml separately. D-Mannitol (50 mM) was used as a standard. The reaction was stopped by the addition of 1 ml of ice-cooled trichloroacetic acid (17.

데이터처리

Statistical analysis - Linear regression analysis was used to calculate the IC50 values. Significance with respect to control was calculated with the help of SPSS 11.

이론/모형

Photochemilumenescence assay - For the determination of the integral antioxidative capacity (AC) of the water sohible substances in HAEt the method of photochemil- uminscence (PCL) was used (Govindarajan et al., 2004a). Apparatus used was Photochem® with Standard kit ACW (Analitik jena AG), where the luminol lays a double role of photosensitizer as well as the radical detecting agent.

후속연구

Thus the varied therapeutic activity of the plant extract may be in part due to its antioxidant activity. The results obtained suggest that H. auriculata possesses potent antioxidant & free radical scavenging activity suggesting that ethnophar-macological approach in selecting the plant for study may be useful. The report of the efficacy of this plant as hepatoprotective and hypoglycaemic may be due to its antioxidant property.

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