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Antifungal Activities of Essential Oils from Glehnia littoralis Alone and in Combination with Ketoconazole

Natural product sciences, v.11 no.2, 2005년, pp.92 - 96  

Shin, Seung-Won (College of Pharmacy, Duksung Women's University)

Abstract AI-Helper 아이콘AI-Helper

The antifungal activities of essential oils from the leaves of Glehnia littoralis, which is cultivated in Korea, were evaluated against pathogenic Trichophyton species by the broth dilution method and the disk diffusion test. Additionally, the effects of the oils together with ketoconazole were test...

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제안 방법

  • Following this, each well was inoculated with 100 pl (approximately 5xl04 CFU/well) of one of the Trichophyton fungal suspensions and cultivated at 25℃. Fractional inhibitory concentrations (FICs) were calculated by dividing the MICs of the oil and ketoconazole combinations by the MICs of the oil or ketoconazole alone. The FIC index, obtained by adding both FICs, was interpreted as indicating a synergistic effect when it was V 0.
  • To evaluate the combined effects of the essential oil compounds, ten serial two-fold dilutions of α-pinene, β-pinene or the essential oil fraction of G. littoralis with the culture medium containing Tween 80 and eight serial two-fold dilutions of ketoconazole with DMSO (dimethyl sulfoxide) were prepared using the same solvents as in the MIC tests. Aliquots of 50 jil of each G littoralis oil dilution were added to the wells of a 96-well plate in a vertical orientation, and 10 jil aliquots of each ketoconazole dilution were added in a horizontal orientation so that the plate contained various concentration combinations of the two compounds.

대상 데이터

  • distributed. Sterile paper disks (8 mm, Advantec, Toyo Roxhi Kaisha) were impregnated with 50 μl of ethanol solutions of the oils (6.25 to 25 mg/disk) and ketoconazole (20-100 μg/disk), and after alcohol evaporation the disks were placed on the culture plates. The diameter of the zone of inhibition (mm) around the disk was measured after cultivation at 25℃ for 72 h.
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참고문헌 (22)

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  16. Miyazawa, M., Kurose, K., Itoh, A., and Hiraoka, N., Comparison of the Essential Oils of Glehnia littoralis from Northern and Southern Japan. J. Agric. Food Chem. 49, 5433-5436 (2001b) 

  17. Patra, M., Shahi, S.K., Midgely, G., and Dikshit, A., Utilization of essential oil as natural antifungal against nail-infective fungi. Flavour Frag. J. 17, 91-94 (2002) 

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  19. Shin, S., Anti-Aspergillus activities of plant essential oils and their combination effects with ketoconazole or amphotericin B. Arch. Pharm. Res. 26, 389-393 (2003) 

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