$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

The mechanism of telomerase regulation is far better understood in mammalian systems than it is in plant systems. In this study, we investigated the possibility that regulation of telomerase activity occurs at the post-transcriptional level in rice plants (Oryza sativa). Specifically, we used RT-PCR with primers based on the rice telomerase reverse transcriptase (OsTERT) cDNA sequence to detect alternative mRNA splicing products. Five types of DNA product that were shorter than predicted by the cDNA sequence were observed for all the tested cells and tissues. Sequencing of these products indicated that they were alternatively spliced OsTERT fragments lacking some portions of the full-length sequence. These results suggest that regulation of telomerase activity occurs at the post-transcriptional level in rice cells and tissues. The relative amounts of full-length OsTERT mRNA and the shorter, alternatively spliced mRNAs were estimated by quantitative RT-Southern analysis. No correlation was found between levels of telomerase activity and accumulation of full-length OsTERT mRNA in the various cell and tissue types, indicating that splicing of OsTERT mRNAs may be important in telomerase regulation in rice. Further studies are necessary to determine whether the spliced OsTERT mRNAs are translated into proteins and if these putative proteins affect telomerase activity.

참고문헌 (44)

  1. Allsop RC, Vaziri H, Ptterson C, Goldstein S, Younglai EV, Futcher AB, Greider CW, Harley CB (1992) Telomere length predicts replicative capacity of human fibroblasts. Proc Natl Acad Sci USA 89: 10114-10118 
  2. Beattie TL, Zhou W, Robinson MO, Harrington L (2001) Functional multimerization of the human telomerase reverse transcriptase. Mol Cell Biol 21: 6151-6160 
  3. EcEaChem MJ, Krauskopf A, Blackburn EH (2000) Telomeres and their control. Annu Rev Genet 34: 331-358 
  4. Greider CW, Blackburn EH (1985) Identification of a specific telomere terminal transferase activity in tetrahymena extracts. Cell 43: 405-413 
  5. Greider CW, Blackburn EH (1987) The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity. Cell 51: 887-898 
  6. Heller K, Kilian A, Piatyszek MA, Kleinhofs A (1996) Telomerase activity in plant extracts. Mol Gen Genet 252: 342-345 
  7. Kilian AD, Bowtell D, Abud HE, Hime GR, Venter DJ, Keese PK, Duncan EL, Reddel RR, Jefferson RA (1997) Isolation of candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types. Hum Mol Genet 6: 2011-2019 
  8. Krauskopf A, Blackburn EH (1998) Rap1 protein regulates telomere turnover in yeast. Proc Nat Acad Sci USA 95: 12486-12491 
  9. Mergny JL, Riou JF, Mailliet P, Paule M, Fichou T, Gilson E (2002) Natural and pharmacological regulation of telomerase. Nucleic Acids Res 30: 839-865 
  10. Modrek B, Lee CA (2002) A genomic view of alternative splicing. Nature Genet 30: 13-19 
  11. Moriarty TJ, Huard S, Dupuis S, Autexier C (2002) Functional multimerization of human telomerase requires an RNA interaction domain in the N terminus of the catalytic subunit. Mol Cell Biol 22: 1253-1265 
  12. Oguchi K, Liu H, Tamura K, Takahashi H (1999) Molecular cloning and charaterization of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana. FEBS Lett 457: 465-469 
  13. Oguchi K, Tamura K, Takahashi H (2004) Charaterization of Oryza sativa telomerase reverse transcriptase and possible role of its phosphorylation in the control of telomerase activity. Gene 342: 57-66 
  14. Ren S, Johnston JS, Shippen DE, Mckinght TD (2004) TELOMERASE ACTIVATOR 1 induces telomerase activity and potentiates responses to auxin in Arabidopsis. Plant Cell 16: 2910-2922 
  15. Richards EJ, Ausubel FM (1988) Isolation of a higher eukaryotic telomere from Arabidopsis thalinana. Cell 53: 127-136 
  16. Ulaner GA, Hu JF, Thanh H, Giudice LC, Hoffman AR (2001) Tissue-specific alternative splicing of human telomerase reverse transcriptase (hTERT) influences telomere lengths during human development. Int J Cancer 91: 644-649 
  17. Kim SH, Kaminker P, Campisi J (2002) Telomeres, aging and cancer; in search of a happy ending. Oncogene 21: 503-511 
  18. van Steensel B, De Lange T (1997) Control of telomere length by the human telomeric protein TRF1. Nature 385: 740-743 
  19. Tamura K, Liu H, Takahashi H (1999) Auxin induction of cell cycle regulated activity of tobacco telomerase. J Biol Chem 274: 20997-21002 
  20. Li Y, Nakazono M, Tsutsumi N, Hirai A (2000) Molecular and cellular characterizations of a cDNA clone encoding a novel isozyme of aldehyde dehydrogenase from rice. Gene 249: 67-74 
  21. Kotoula V, Barbanis S, Nikolakaki E, Koufoyannis D, Papadimitiou CS, Karkavelas G (2004) Relative expression of human telomerase catalytic subunit (hTERT) transcripts in astrocytic gliomas. Acta Neuropathol 107: 443-451 
  22. Yi X, White DM, Aisner DL, Baur JA, Wright WE, Shay JW (2000) An alternative splicing variant of the human telomerase catalytic subunit inhibits telomerase activity. Neoplasia 2: 433-440 
  23. Ulaner GA, Hu JF, Vu TH, Giudice LC, Hoffman AR (1998) Telomerase activity in human development is regulated by human telomerae reverse transcriptase (hTERT) transcription and by alternate splicing of hTERT transcripts. Cancer Res 58: 4168-4172 
  24. Klapper W, Shin T, Mattson MP (2001) Differential regulation of telornerase activity and TERT expression during brain development in mice. J Neurosci Res 64: 252260 
  25. Meyne J, Ratliff RL, Moyzis RK (1989) Conservation of human telomere sequence (TIAGGG)n among vertebrates. Proc Natl Acad Sci USA 86: 7049-7053 
  26. Moriarty TJ, Marie-Egyptienne DT, Autexier C (2004) Functional organization of repeat addition processivity and DNA synthesis determinants in the human telomerase multimer. Mol Cell Biol 24: 3720-3733 
  27. Morin GB (1989) The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TIAGGG repeats. Cell 59: 521-529 
  28. Ulaner GA, Hu JF, Vu TH, Oruganti H, Giudice LC, Hoffman AR (2000) Regulation of telomerase by alternative splicing of human telomerase reverse transcriptase (hTERT) in normal and neoplastic ovary, endometrium and myometrium. Int J Cancer 85: 330-335 
  29. Xia J, Peng Y, Mian IS, Lue NF (2000) Identification of functionally important domains in the N-terminal region of telomerase reverse transcriptase. Mol Cell Biol 20: 5196-5207 
  30. Fitzgerald MS, McKnight TD, Shippen DE (1996) Characterization and developmental patterns of telomerase expression in plants. Proc Natl Acad Sci USA 93: 14422-14427 
  31. Kipling D (1995) The Telomere. Oxford University Press, Tokyo, pp 31-58 
  32. Heller-Uszynska K, Schnippenkoetter W, Kilian A (2002) Cloning and charaterization of rice (Oryza sativa L) telomerase reverse transcriptase, which reveals complex splicing patterns. Plant J 31: 75-86 
  33. Yang SE, Jin ES, Chung IK, Kim WT (2002) Cell cycledependent regulation of telomerase activity by auxin, abscisic acid and protein phosphorylation in tobacco BY-2 suspension culture cells. Plant J 29: 617-626 
  34. Adams MD, Rudner DZ, Rio DC (1996) Biochemistry and regulation of pre-mRNA splicing. Curr Opinion Cell Biol 8: 331-339 
  35. Prescott A, Martin CA (1987) A rapid method for the quantitative assessment of levels of specific mRNAs in plants. Plant Mol Biol Rep 4: 219-224 
  36. Olovnikov AM (1973) A theory of marginotomy. The incomplete copying of template margin in enzymatic synthesis of polynucleotides and biological significance of the phenomenun. J Theor Biol 41: 181-190 
  37. Sanger FS, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitor. Proc Natl Acad Sci USA 74: 5463-5467 
  38. Henderson ER, Blackburn EH (1989) An overhanging 3' terminus is a conserved feature of telomeres. Mol Cell Biol 9: 345-348 
  39. Chung H, Hirata Y, Ando S, Kamachi S, Sakai S (2003) Mechanism regulating telomerase activity in Oryza sativa L. Plant Sci 165: 43-54 
  40. Colgin LM, Wilkison C, Engiezou A, Kilian A, Robinson MO, Reddle RR (2000) The hTEP a splice variant is a dominant negative inhibitor of telomerase activity. Neoplasia 2: 426-432 
  41. Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18: 659-668 
  42. Yang SE, Kim SK, Kim WT (2004) Pertubation of NgTRF1 expression induces apoptosis-like cell death in tobacco BY-2 cells and implicates NgTRF1 in the control of telomere length and stability. Plant Cell 16: 3370-3385 
  43. Tesmer VM, Ford LP, Holt SE, Frank BC, Yi X, Aisner DL, Ouellette M, Shay JW, Wright WE (1999) Two active fragments of the integral RNA cooperate to assemble active telomerase with the human protein catalytic subunit (DTERT) in vitro. Mol Cell Biol 19: 6207-6216 
  44. Woodley L, Valcarcel J (2002) Regulation of alternative premRNA splicing. Brif Funct Genomic Proteomic 1; 266-277 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

원문보기

원문 PDF 다운로드

  • 원문 PDF 정보가 존재하지 않습니다.

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일