$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

Brassinosteroid (BR)-6-oxidases mediate the bridge reactions that connect the late and early C-6 oxidation pathways by converting 6-deoxoBR to 6-oxoBRs. Two similar genes of Arabidopsis, CYP85A1 (At5g38970) and CYP85A2 (At3g30180), are proposed to encode BR-6-oxidases based on findings that heterologously expressed genes mediate BR-6-oxidation reactions in yeast. However, genetic evidence that both genes are critically involved in the BR-6-oxidation step in Arabidopsis has been limited. Here, we show that a double mutant for the two genes displays dwarfism similar to that of typical BR biosynthesis-deficient mutants, suggesting that they are the major BR-6-oxidases in Arabidopsis. Examination of endogenous BR levels and metabolism monitoring tests using this double mutant revealed a great reduction in the levels of 6-oxoBRs, e.g., TY and CS, due to a lack in the conversion reactions from 6-deoxoCS to CS, and from 6-deoxoTY to TY. Surprisingly, the double mutant accumulated a significant amount of 6-oxocampestanol, suggesting that the upstream C-6 oxidation of campestanol to 6-oxocampestanol is not catalyzed by the two BR-6-oxidases in Arabidopsis, rather, by another enzyme yet to be discovered.

참고문헌 (19)

  1. Asami T, Mizutani M, Fujioka S, Goda H, Min YK, Shimada Y, Nakano T, Takatsuto S, Matsuyama T, Nagata N, Sakata K, Yoshida S (2001) Selective interaction of triazole derivatives with DWF4, a cytochrome P450 monooxygenase of the brassinosteroid biosynthetic pathway, correlates with brassinosteroid deficiency in planta. J Biol Chem 276: 25687-25691 
  2. Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA (2001) Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. Plant J 26: 573-582 
  3. Kwon M, Lee HK, Choe S (2005) Novel simple sequence length polymorphic (SSLP) markers for positional cloning in Arabidopsis thaliana. Kor J Genet 27: 1-8 
  4. Kang JG, Yun J, Kim DH, Chung KS, Fujioka S, Kim JI, Dae HW, Yoshida S, Takatsuto S, Song PS, Park CM (2001) Light and brassinosteroid signals are integrated via a dark-induced small G protein in etiolated seedling growth. Cell 105: 625-636 
  5. Shimada Y, Coda H, Nakamura A, Takatsuto S, Fujioka S, Yoshida S (2003) Organ-specific expression of brass inosteroid-biosynthetic genes and distribution of endogenous brassinosteroids in Arabidopsis. Plant Physiol 131: 287-297 
  6. Nomura T, Kushiro T, Yokota T, Kamiya Y, Bishop GJ, Yamaguchi S (2005) The last reaction producing brassinolide is catalyzed by cytochrome P450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis. J Biol Chem 280: 17873-17879 
  7. Fujioka S, Noguchi T, Watanabe T, Takatsuto S, Yoshida S (2000) Biosynthesis of brassinosteroids in cultured cells of Catharanthus roseus. Phytochem 53: 549-553 
  8. Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301: 653-657 
  9. Kwon M, Choe S (2005) Brassinosteroid biosynthesis and dwarf mutant. J Plant Biol 48: 1-15 
  10. Hong Z, Ueguchi-Tanaka M, Shimizu-Sato S, Inukai Y, Fujioka S, Shimada Y, Takatsuto S, Agetsuma M, Yoshida S, Watanabe Y, Uozu S, Kitano H, Ashikari M, Matsuoka M (2002) Loss-of-function of a rice brassinosteroid, biosynthetic enzyme, C-6 oxidase, prevents the organized arrangement and polar elongation of cells in the leaves and stem. Plant J 32: 495-508 
  11. Shimada Y, Fujioka S, Miyauchi N, Kushiro M, Takatsuto S, Nomura T, Yokota T, Kamlya Y, Bishop GJ, Yoshida S (2001) Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis. Plant Physiol 126: 770-779 
  12. Nomura T, Sato T, Bishop GJ, Kamiya Y, Takatsuto S, Yokota T (2001) Accumulation of 6-deoxocathasterone and 6-deoxocastasterone in Arabidopsis, pea and tomato is suggestive of common rate-limiting steps in brassinosteroid biosynthesis. Phytochem 57: 171-178 
  13. Hong,Z, Ueguchi-Ianaka M, Umemura K, Uozu S, Fujioka S, Takatsuto S, Yoshida S, Ashikari M, Kitano H, Matsuoka M (2003) A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell 15: 2900-2910 
  14. Choe S (2005) Brassinosteroid biosynthesis and metabolism. In Davies PJ, ed, Plant Hormones: Biosynthesis, Signal Transduction, and Action. Springer, Syracus, pp 156-178 
  15. Choe S, Noguchi T, Fujioka S, Takatsuto S, Tissier C, Gregory B, Ross A, Tanaka A, Yoshida S, Tax F, Feldmann KA (1999) The Arabidopsis dwf7/ste1 mutant is defective in the delta 7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis. Plant Cell 11 : 207-221 
  16. Noguchi T, Fujioka S, Choe S, Takatsuto S, Yoshida S, Yuan H, Feldmann KA, Tax FE (1999) Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids. Plant Physiol 121: 743-752 
  17. Bishop GJ, Harrison K, Jones JD (1996) The tomato Dwarf gene isolated by heterologous transposon tagging encodes the first member of a new cytochrome P450 family. Plant Cell 8: 959-969 
  18. Bishop GJ, Nomura T, Yokota T, Harrison K, Noguchi T, Fujioka S, Takatsuto S, Jones JD,.Kamiya Y (1999) The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis. Proc Natl Acad Sci USA 96: 1761-1766 
  19. Noguchi T, Fujioka S, Choe S, Takatsuto S, Tax FE, Yoshida S, Feldmann KA (2000) Biosynthetic pathways of brassinolide in Arabidopsis. Plant Physiol 124: 201-209 

이 논문을 인용한 문헌 (2)

  1. 2007. "" Journal of plant biology = 식물학회지, 50(3): 225~229 
  2. 2011. "" Journal of plant biology = 식물학회지, 54(1): 10~14 

원문보기

원문 PDF 다운로드

  • 원문 PDF 정보가 존재하지 않습니다.

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일