Abstract

Lead and cadmium are potent environmental toxicants that affect populations living in Europe. Americas, and Asia. Identifying transporters for lead and cadmium could potentially 1 help us better understand possible risk factors. The iron transporter, divalent metal transporter 1 (DMT1), mediates intestinal transport of cadmium, and lead in yeast and fobroblasts overexpressing DMT1. In human intestinal cells knocking down expression of DMT1 attenuated uptake of cadmium and iron but not lead. A possible explanation is the expression of a second transporter for lead in intestine. In astrocytes, however, DMT1 appears to transport lead in an extracellular buffer at pH value. At neutral pH, transport was not mediated by DMT1 but rather by a transporter that is stimulated by bicarbonate and inhibited by 4,4'-diisothiocyanatodihydrostilbene-2,2'-disulfonic acid. The identity of this lead transporter will beverified by future study.

주제어

#divalent metal transporter 1 (DMT1)   #cadmium   #lead  

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참고문헌 (57)

1. Alper, S.L. (1998) The band 3-related AE anion exchange gene family. Cell Physiol. Biochem. 4, 265-281 
2. Alper, S.L., et al. (2002) The AE gene family of Cl/HCO3-exchangers. J. Nephrol. 15 Suppl 5, S41-53 
3. Bannon, D.I., Abounader, R., Lees, P.S.J. and Bressler, J.P. (2003b) Effect of DMTl Knockdown on iron, cadmium and lead uptake in Caco-2 cells. Am. J. Psysiol. 28, C44-50 
4. Bannon, D.I., et al. (2002) Uptake of lead and iron by divalent metal transporter 1 in yeast and mammalian cells. Biochem. Biophys. Res. Commun. 295(4), 978-984 
5. Bannon, D.I., et al. (2003a) Effect of DMTl knockdown on iron, cadmium, and lead uptake in Caco-2 cells. Am. J. Physiol. Cell. Physiol. 284(1), C44-50 
6. Bellinger, D. (1994) Low-level lead exposure and childrens cognitive function in the preschool years. Peds. 93(2), A28 
7. Berglund, M., et al. (1994) Intestinal absorption of dietary cadmium in women depends on body iron stores and fiber intake. Environ. Health Perspect. 102(12), 1058-1066 
8. Bevensee, M.O., Weed, R.A. and Boron, W.F. (1997b) Intracellular pH regulation in cultured astrocytes from rat hippocampus. I. Role Of HCO3. J. Gen. Physiol. 110(4), 453-465 
9. Canfield, R.L., et al. (2003) Intellectual impairment in children with blood lead concentrations below 10 microg per deciliter. N. Engl. J. Med. 348(16), 1517-1526 
10. Cerklewski, F.L. and Forbes, R.M. (1976) Influence of dietary zinc on lead toxicity in the rat. J. Nutr. 106(5), 689-696 
11. Cheng, Y, et al. (2001) Bone lead and blood lead levels in relation to baseline blood pressure and the prospective development of hypertension: the Normative Aging Study. Am. J. Epidemiol. 153(2), 164-171 
12. Cheong, J.H., Bannon D.I., Olivi L., Kim, Y.B. and Bressler, J.P. (2004) Different Mechanisms Mediate Uptake of Lead in a Rat Astroglial Cell Line. Toxicol. Sci. 77, 334-340 
13. Cobbett, C.S. (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol. 123(3), 825-832 
14. de Kloet, E.R. and M.S. Oitzl (2003) Who cares for a stressed brain? The mother, the kid or both? Neurobiol. Aging 24 Suppl 1, S61-65; discussion S67-68 
15. Donovan, A., et al. (2000) Positional cloning of zebrafish ferroportinl identifies a conserved vertebrate iron exporter [see comments]. Nature 403(6771), 776-781 
16. Drasch, G.A., et al. (1983) An increase of cadmium body burden for this century--an investigation on human tissues. Sci. Total Environ. 26(2), 111-119 
17. Eide, D.J. (2004) The SLC39 family of metal ion transporters. Pflugers Arch. 447(5), 796-800 
18. el-Waseef, A. and Hashim, M.M. (1985) Zinc lead-interaction in the rabbit. Acta. Med. Hung. 42(3-4), 199-207 
19. Flanagan, P.R., et al. (1978) Increased dietary cadmium absorption in mice and human subjects with iron deficiency. Gastroenterology. 74(5 Pt 1), 841-846 
20. Fleming, M.D., et al. (1998) Nramp2 is mutated in the anemic Belgrade (b) rat: evidence of a role for Nramp2 in endosomal iron transport. Proc. Natl. Acad. Sci. 95(3), 1148-1153 
21. Gaither, L.A. and Eide, D.J. (2000) Functional expression of the human hZIP2 zinc transporter. J. Biol. Chem. 275(8), 5560-5564 
22. Guerinot, M.L. (2000) The ZIP family of metal transporters. Biochim. Biophys. Acta. 1465(1-2), 190-198 
23. Gunshin, H., et al. (1997) Cloning and characterization of a mam-malian proton-coupled metal-ion transporter. Nature 388(6641), 482-488 
24. Hediger, M.A., et al. (2004) The ABCs of solute carriers: physiological, pathological and therapeutic implications of human membrane transport proteinsIntroduction. Pflugers Arch. 447(5), 465-468 
25. Hertz-Picciotto, I. and Croft, J. (1993) Review of the relation between blood lead and blood pressure. Epidemiol. Rev. 15(2), 352-373 
26. Holtzman, D., et al.(1984) Maturation of resistance to lead encephalopathy: Cellular and subcellular mechanisms. Neurotoxicology 5(3), 97-124 
27. Lal, B., Goldstein, G.W. and Bressler, J.P. (1996) Role of anion exchange and thiol groups in the regulation of potassium efflux by lead in human erythrocytes. J. Cell. Phys. 167, 222-228 
28. Lou, M., Garay, R. and Aida, J.O. (1991) Cadmium uptake through the anion exchanger in human red blood cells. J. Physiology 443, 123-136 
29. Lupien, S.J., et al. (2000) Child's stress hormone levels correlate with mother's socioeconomic status and depressive state. Biol. Psychiatry 48(10), 976-980 
30. Markovich, D. and Murer, H. (2004) The SLC13 gene family of sodium sulphate/carboxylate cotransporters. Pflugers Arch. 447(5), 594-602 
31. Mount, D.B. and Romero M.F. (2004) The SLC26 gene family of multifunctional anion exchangers. Pflugers Arch. 447(5), 710-721 
32. Olson, C.M. (1999) Nutrition and health outcomes associated with food insecurity and hunger. J. Nutr. 129(2S Suppl), 521S-524S 
33. Oomen, A.G., et al. (2003) In vitro intestinal lead uptake and transport in relation to speciation. Arch. Environ. Contam. Toxicol. 44(1), 116-124 
34. Paschal, D.C., et al. (2003) Exposure of the U.S. population aged 6 years and older to cadmium: 1988- 1994. Arch. Environ. Contam. Toxicol. 38(3), 377-383 
35. Ragan, H.A. (1977) Effects of iron deficiency on the absorption and distribution of lead and cadmium in rats. J. Lab. Clin. Med. 90(4), 700-706 
36. Satarug, S., Haswell-Elkins, M.R. and Moore, M.R. (2000) Safe levels of cadmium intake to prevent renal toxicity in human subjects. Br. J. Nutr. 84(6), 791-802 
37. Schopfer, L.M. and Salhany, J.M. (1995) Characterization of the stilbenedisulfonate binding site on band 3. Biochemistry 34(26), 8320-8329 
38. Selevan, S.G., et. al. (2003) Blood lead concentration and delayed puberty in girls. N. Engl. J. Med. 348(16), 1527-1536 
39. Simpson, R.J. and Peters, T.J. (1986) Mouse intestinal Fe3+ uptake kinetics in vivo. The significance of brush-border membrane vesicle transport in the mechanism of mucosal Fe3+ uptake. Biochim. Biophys. Acta., 856(1), 115-122 
40. Stanek, E.J., 3rd and Calabrese, E.J. (2000) Daily soil ingestion estimates for children at a Superfund site. Risk Anal. 20(5), 627-635 
41. Stewart, W.F., et al. (1999) Neurobehavioral function and tibial and chelatable lead levels in 543 former organolead workers. Neurology 52(8), 1610-1617 
42. Sugawara, N. and Sugawara, C. (1991) Gastrointestinal absorption of Cd-metallothionein and cadmium chloride in mice. Arch. Toxicol. 65(8), 689-692 
43. Trinder, D., et al. (2000) Localization of divalent metal transporter 1 (DMT1) to the microvillus membrane of rat duodenal enterocytes in iron deficiency, but to hepatocytes in iron overload [see comments]. Gut 46(2), 270-276 
44. Vahter, M., et al. (1996) Bioavailability of cadmium from shellfish and mixed diet in women. Toxicol. Appl. Pharmacol. 136(2), 332-341 
45. Valberg, L.S., Sorbie, J. and Hamilton, D.L. (1976) Gastrointestinal metabolism of cadmium in experimental iron deficiency. Am. J. Physiol. 231(2), 462-467 
46. Vulpe, C.D., et al. (1999) Hephaestin, a ceruloplasmin homologue implicated in intestinal iron transport, is defective in the sla mouse. Nat. Genet. 21(2), 195-199 
47. Calabrese, E.J., et al. (1997) Soil ingestion: a concern for acute toxicity in children. Environ Health Perspect, 1997. 105(12), 1354-1358 
48. Dufner-Beattie, J., et al. (2003) The Acrodermatitis Enteropathica Gene ZIP4 Encodes a Tissue-specific, Zinc-regulated Zinc Transporter in Mice. J. Biol. Chem. 278(35), 33474-33481 
49. Bebe, F.N. and Panemangalore, M. (1996) Modulation of tissue trace metal concentrations in weanling rats fed different levels of zinc and exposed to oral lead and cadmium. Nutrition Res. 16(8), 1369-1380 
50. Crowe, A. and Morgan E.H. (1996) Interactions between tissue uptake of lead and iron in normal and iron-deficient rats during development. Biol. Trace Elem. Res. 52(3), 249-261 
51. Groten, J.P., Luten, J.B. and van Bladeren, P.J. (1992) Dietary iron lowers the intestinal uptake of cadmium-metallothionein in rats. Eur. J.Pharmacol. 228(1), 23-28 
52. Klein, D., Greim, H. and Summer K.H. (1986) Stability of metallothionein in gastric juice. Toxicology 41(2), 121-129 
53. Simons, T.J.B. (1986) The role of anion transport in the passive movement of lead across the human red cell membrane. J. Physiol. (Land) 378, 287-312 
54. Bevensee, M.O., Apkon, M. and Boron, W.F. (1997a) Intracellular pH regulation in cultured astrocytes from rat hippocampus. II. Electrogenic Na/HCO3 cotransport. J. Gen. Physiol. 110(4), 467-483 
55. Jarup, L., et al. (2000) Low level exposure to cadmium and early kidney damage: The OSCAR study. Occup. Environ. Med. 57 (10), 668-672 
56. Ohta, H. and Cherian, M.G. (1991) Gastrointestinal absorption of cadmium and metallothionein. Toxicol. Appl.Pharmacol. 107(1), 63-72 
57. Romero, M.F., Fulton, C.M. and Boron, W.F. (2004) The SLC4 family of HCO3 - transporters. Pflugers Arch. 447(5), 495-509 

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