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Abstract

The biosynthetic gene cluster for the aminoglycoside antibiotic kasugamycin was isolated and characterized from the kasugamycin producing strain, Streptomyces kasugaensis KACC 20262. By screening a fosmid library using kasA, the gene encoding aminotransferase, we isolated a 22 kb DNA fragment. The fragment contained seventeen complete open reading frames (ORFs); one of these ORFs, kasD, was identified as the gene for dNDP-glucose 4,6-dehydratase, which catalyzes the conversion of dNDP-glucose to 4-keto-6-deoxy-dNDP-glucose. The enzyme showed a broad spectrum of substrate specificity. In addition, ksR was overexpressed in E. coli BL21 and proved to be a self-resistance gene against kasugamycin. These findings suggest that the isolated gene cluster is highly likely responsible for the biosynthesis of kasugamycin.

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이 논문을 인용한 문헌 (5)

  1. 2006. "" Journal of microbiology and biotechnology, 16(12): 1912~1918 
  2. 2006. "" Journal of microbiology and biotechnology, 16(6): 927~932 
  3. 2006. "" Journal of microbiology and biotechnology, 16(8): 1311~1315 
  4. 2006. "" Journal of microbiology and biotechnology, 16(9): 1472~1476 
  5. 2007. "" Journal of microbiology and biotechnology, 17(4): 685~690 

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