• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


From a genomic library of Lactobacillus reuteri ATCC 55739, one clone, NE347, carrying a pyrH gene encoding UMP kinase, was identified. pNE347 carried a 1.88 kb EcoRI fragment and the pyrH was located in the middle of the insert. pyrH ORF was 723 bp in size and capable of encoding UMP kinase composed of 240 amino acid residues. tsf encoding an elongation factor-Ts and frr encoding a ribosomal recycling factor were present upstream and downstream of pyrH, respectively. When introduced into E. coli KUR1244, a pyrH-negative strain, pNE347 restored the ability to grow at $42^{\circ}C$, indicating that pyrH from L. reuteri synthesized functional UMP kinase in E. coli. Northern blot experiment showed that pyrH and frr were cotranscribed as a 1.4 kb single transcript. pyrH was overexpressed in E. coli by using a pET26b(+) vector, and a major 25 kDa protein band appeared on SDS-polyacrylamide gel.

참고문헌 (17)

  1. Ingraham, J. L. and J. Neuhard. 1972. Cold-sensitive mutants of Salmonella typhimurium defective in uridine monophosphate kinase. J. Biol. Chem. 247: 6259-6265 
  2. Laemmli, U. K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680-685 
  3. Park, R.-J., K.-H. Lee, S.-J. Kim, J.- Y. Park, S.-J. Nam, H.-D. Yun, H.-J. Lee, H. C. Chang, D. K. Chung, J.-H. Lee, Y H. Park, and J. H. Kim. 2002. Isolation of Lactococcus lactis strain with ${\beta}$-galactosidase activity trom kimchi and cloning of lacZ gene trom the isolated strain. J. Microbiol. Biotechnol. 12: 157-161 
  4. Serina, L., C. Blondin, E. Krin, O. Sismeiro, A. Danchin, H. Sakamoto, A.-M. Gilles, and O. Barzu. 1995. Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP. Biochemistry 34: 5066-5074 
  5. Smallshaw, J. and R. A. Kelln. 1992. Cloning, nucleotide sequence and expression of the Escherichia coli K-12 pyrH gene encoding UMP kinase. Genetics (Life Sci. Adv.) 11: 59-65 
  6. Wadskov-Hansen, S. L., J. Martinussen, and K. Hammer. 2000. The pyrH gene of Lactococcus lactis subsp. cremoris encoding UMP kinase is transcribed as part of an operon including the frr1 gene encoding ribosomal recycling factor 1. Gene 241: 157-166 
  7. Ohnishi, M., L. Janosi, M. Shuda, H. Matsumoto, T. Hayashi, Y. Terawaki, and A. Kaji. 1999. Molecular cloning, sequencing, purification, and characterization of Pseudomonas aeruginosa ribosome recycling factor. J. Bacteriol. 181: 1281-1291 
  8. Bucurenci, N., L. Serina, C. Zaharia, S. Landais, A. Danchin, and O. Barzu. 1998. Mutational analysis of UMP kinase from Escherichia coli. J. Bacteriol. 180: 473-477 
  9. Saavedra, J. 2000. Probiotics and infectious diarrhea. Am. J. Gastroenterol. 95: S16-S18 
  10. Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16: 6127-6145 
  11. Talarico, T. L., I. A. Casas, T. C. Chung, and W. J. Dobrogosz. 1988. Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri. Antimicrob. Agents Chemother. 32: 1854-1858 
  12. Lee, K. H., G. S. Moon, J. Y. An, H. J. Lee, H. C. Cahng, D. K. Chung, J. H. Lee, and J. H. Kim. 2002. Isolation of a nisin-producing Lactococcus lactis strain trom kimchi and characteriaztion of its nisZ gene. J. Microbiol. Biotechnol. 12: 389-397 
  13. Jeong, S. J., D. J. You, H. J. Kwon, S. Kanaya, N. Kunihiro, K. H. Kim, Y. H. Kim, and B. W. Kim. 2002. Cloning and characterization of cycloinulooligosaccharide fructanotransferase (CFTase) from Bacillus polymyxa MGL21. J. Microbiol. Biotechnol. 12: 921-928 
  14. Kim, T. Y., J. M. Lee, H. C. Chang, D. K. Chung, J. H. Lee, J. H. Kim, and H. J. Lee. 1999. Effect of temperature and a carbon source on the expression of ${\beta}$-galactosidase gene of Lactococcus lactis ssp. lactis ATCC 7962. J. Microbiol. Biotechnol. 9: 201-205 
  15. Blank, J., S. Nock, R. Kreutzer, and M. Sprinzl. 1996. Elongation factor Ts from Thermus thermophilus overproduction in Escherichia coli, quaternary structure and interaction with elongation factor Tu. Eur. J. Biochem. 236: 222-227 
  16. Park, J. Y., S. J. Park, S. J. Nam, Y. L. Ha, and J. H. Kim. 2002. Cloning and characterization of L-Iactate dehydrogenase gene (ldhL) trom Lactobacillus reuteri ATCC 55739. J. Microbiol. Biotechnol. 12: 716- 721 
  17. Sambrook, J. E. F. Fritsch, and J. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., U.S.A 

이 논문을 인용한 문헌 (1)

  1. 2006. "" Journal of microbiology and biotechnology, 16(6): 988~992 


원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일