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In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide ($10\;{\mu}g/ml$), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with $IC_{50}$ value of $3.0\;{\mu}g/ml$ after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.

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이 논문을 인용한 문헌 (6)

  1. 2006. "" Journal of microbiology and biotechnology, 16(3): 475~479 
  2. 2006. "" Journal of microbiology and biotechnology, 16(8): 1262~1268 
  3. 2007. "" Journal of microbiology and biotechnology, 17(11): 1856~1861 
  4. 2007. "" Journal of microbiology and biotechnology, 17(4): 638~643 
  5. 2008. "" Journal of microbiology and biotechnology, 18(3): 523~531 
  6. 2009. "" Journal of microbiology and biotechnology, 19(9): 911~917 


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