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논문 상세정보

Abstract

Subtilisin (EC 3.4.21.14) is the major extracellular alkaline serine protease of Bacillus species. Previously, we found that subtilisins did not migrate in the electrophoretic field in the Laemmili buffer system due to their high pI values (over 8.8); however, it formed a 'binding mode' at the top of the separating gel [5]. Utilizing this characteristic, four subtilisins from Bacillus sp. strains (e.g., B. subtilis 168, B. subtilis KCTC 1021, B. amyloliquefaciens KCTC 3002, and Bacillus sp. DJ-1 and DJ-4) were easily and quickly identified by an over-running electrophoretic technique with a miniscale culture supernatant (less than 20 ml) without any column chromatographic steps. Two subtilisins (DJ-l and a recombinant version) from Bacillus sp. DJ-l were characterized, and the enzymatic properties were determined by SDS-fibrin zymography and densitometric analysis. Based on this observation, the recombinant pro-subtilisin DJ-l showed the same 'binding mode,' similar to native subtilisin DJ-l. On the other hand, mature subtilisin DJ -1 without pro-peptide showed no enzymatic activity.

참고문헌 (37)

  1. Brown, T. L., M. G. Yet, and F. Wold. 1982. Substrate-containing gel electrophoresis: Sensitive detection of amylolytic, nucleolytic, and proteolytic enzymes. Anal. Biochem. 122: 164-172 
  2. Choi, N. S., K. H. Yoo, K. S. Yoon, K. T. Chang, P. J. Maeng, and S. H. Kim. 2004. Identification of recombinant subtilisins. J. Microbiol. Biotechnol. 15: 35-39 
  3. Choi, N. S., K. T. Chang, P. J. Maeng, and S. H. Kim. 2004. Cloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene. from Bacillus sp. DJ-4. FEMS Microbiol. Lett. 236: 325-331 
  4. Kim, S. H. and N. S. Choi. 1999. Electrophoretic analysis of protease inhibitors in fibrin zymography. Anal. Biochem. 270: 179-181 
  5. Kim, W., K. Choi, Y. Kim, H. Park, J. Choi, Y. Lee, H. Oh, I. K won, and S. Lee. 1996. Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang. Appl. Environ. Microbiol. 62: 2482-2488 
  6. Ko, J. H., J. P. Peng, J. Zhu, and Y. P. Qi. 2004. Identification of two novel fibrinolytic enzymes from Bacillus subtilis QK02. Comp. Biochem. Physiol. C 137: 65-74 
  7. Kurihara, M., F. S. Markland, and E. L. Smith. 1972. Subtilisin Amylosacchariticus. 3. Isolation and sequence of the chymotryptic peptides and the complete amino acid sequence. J. Biol. Chem. 247: 5619-5631 
  8. Peng, Y., Q. Huang, R. Zhang, and Y. Zhang. 2003. Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 134: 45-52 
  9. Priest, F. G. 1977. Extracellular enzyme synthesis in the genus Bacillus. Bacteriol. Rev. 41: 711-753 
  10. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 
  11. Smith, E. L., R. J. Delange, W. H. Evans, M. Landon, and F. S. Markland. 1968. Subtilisin Carlsberg. V. The complete sequence; comparison with subtilisin BPN'; evolutionary relationships. J. Biol. Chem. 243: 2184-2191 
  12. Chang, C. T., M. H. Fan, F. C. Kuo, and H. Y. Sung. 2000. Potent fibrinolytic enzyme from a mutant of Bacillus subtilis IMR-NK1. J Agric. Food Chem. 48: 3210-3216 
  13. Kamal, M., J. Hoog, R. Kaiser, J. Shafqat, T. Razzaki, Z. H. Zaidi, and H. Jornvall. 1995. Isolation, characterization and structure of subtilisin from thermostable Bacillus subtilis isolate. FEBS Lett. 374: 363-366 
  14. Kim, S. H., N. S. Choi, and W. Y. Lee. 1998. Fibrin zymography: A direct analysis of fibrinolytic enzymes on gels. Anal. Biochem. 263: 115-116 
  15. Rochelle, P. A., J. C. Fry, R. J. Parkes, and A. J. Weightman. 1992. DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. FEMS Microbiol. Lett. 100: 59-66 
  16. Nakamura, T., Y. Yamagata, and E.lchishima. 1992. Nucleotide sequence of the subtilisin NAT gene, aprN, of Bacillus subtilis (natto). Biosci. Biotechnol. Biochem. 56: 1869-1871 
  17. Deutscher, M. P. 1990. Guide to Protein Purification. Academic Press, San Diego 
  18. Matsubara, H., C. B. Kasper, D. M. Brown, and E. L. Smith. 1965. Subtilisin BPN. I. Physical properties and amino acid composition. J. Biol. Chem. 240: 1125-1130 
  19. Wells, J. A. and D. A. Estell. 1988. Subtilisin - an enzyme designed to be engineered. Trends Biochem. Sci. 13: 291-297 
  20. Lantz, M. S. and P. Ciborowski. 1994. Zymographic techniques for detection and characterization of microbial proteases. Methods Enzymol. 235: 563-594 
  21. Jang, J. S., D. O. Kang, M. J. Chun, and S. M. Byun. 1992. Molecular cloning of a subtilisin J. gene from Bacillus stearothermophilus and its expression in Bacillus subtilis. Biochem. Biophys. Res. Commun. 184: 277-282 
  22. Ikemura, H. and M. Inouye. 1988. In vitro processing of prosubtilisin produced in Escherichia coli. J. Biol. Chem. 263: 12959-12963 
  23. Kim, S. H. and N. S. Choi. 2000. Purification and characterization of subtilisin DJ-4 secreted by Bacillus sp. strain DJ-4 screened from Doen-Jang. Biosci. Biotechnol. Biochem. 64: 722-1725 
  24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. CSH, New York, U.S.A 
  25. Simonen, M. and J. Palva. 1993. Protein secretion in Bacillus species. Microbiol. Rev. 57: 109-137 
  26. Choi, N. S. and S. H. Kim. 2000. Two fibrin zymography methods for analysis of plasminogen activators on gels. Anal. Biochem. 281: 236-238 
  27. Kim, J. S., I. S. Lee, S. Lee, Y. P. Lee, C. Jung, H. C. Kim, and S. Y. Choi. 2002. Cloning and expression of the aminopeptidase gene from the Bacillus licheniformis in Bacillus subtilis. J. Microbiol. Biotechnol. 12: 773-779 
  28. Gharahdaghi, F., M. Kirchner, J. Fernandez, and S. M. Mische. 1996. Peptide-mass profiles of polyvinylidene difluoride-bound proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in the presence of nonionic detergents. Anal. Biochem. 233: 94-99 
  29. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 
  30. Fujita, M., K. Nomura, K. Hong, Y Ito, A. Asada, and S. Nishimuro. 1993. Purification and characterization of a string fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan. Biochem. Biophys. Res. Commun. 197: 13470-13472 
  31. Gharahdaghi, F., C. R. Weinberg, D. A. Meagher, B. S. Imai, and S. M. Mische. 1999. Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity. Electrophoresis 20: 601-605 
  32. Wong, S. L., C. W. Price, D. S. Goldfarb, and R. H. Doi. 1984. The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo. Proc. Natl. Acad. Sci. USA 81: 1184-1188 
  33. Yamazaki, H., K. Ohmura, A. Nakayama, Y. Takeichi, K. Otozai, M. Yamasaki, G. Tamura, and K. Yamane. 1983. Alpha-amylase genes (amyR2 and $amyE^+$) from an alpha-amylase-hyperproducing Bacillus subtilis strain: Molecular cloning and nucleotide sequences. J. Bacteriol. 156: 327-337 
  34. Choi, N. S., J. Y. Lee, K. S. Yoon, K. Y. Han, and S. H. Kim. 2001. Identification of total extracellular fibrinase from Bacillus sp. DJ using one- or two-dimensional fibrin zymography for proteomic approach. J. Microbiol. Biotechnol. 11: 1111-1114 
  35. Zhu, X. L., Y. Ohta, F. Jordan, and E. Inouye. 1989. Prosequence of subtilisin can guide the refolding of denatured subtilisin in an intermolecular process. Nature 339: 483-484 
  36. Kaneko, R., N. Koyama, Y. C. Tsai, R. Y Juang, K. Yoda, and M. Yamasaki. 1989. Molecular cloning of the structural gene for alkaline elastase YaB, a new subtilisin produced by an alkalophilic Bacillus strain. J. Bacteriol. 171: 5232-5236 
  37. Takagi, H., M. Yamamoto, I. Ohtsu, and S. Nakamori. 1998. Random mutagenesis into the conserved Gly 154 of subtilisin E: Isolation and characterization of the revertant enzymes. Protein Eng. 11: 1205-1210 

이 논문을 인용한 문헌 (9)

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  6. Cho, Eun-Kyung ; Jung, Yu-Jung ; Gal, Sang-Wan ; Choi, Young-Ju 2009. "Isolation and Characterization of Bacillus licheniformis SC082 Degrading Fibrin and Chitin from Shrimp Jeot-Gal" 생명과학회지 = Journal of life science, 19(10): 1424~1431 
  7. 2009. "" Journal of microbiology and biotechnology, 19(9): 997~1004 
  8. 2011. "" Journal of microbiology and biotechnology, 21(11): 1166~1173 
  9. Jo, Hyeon-Deok ; Kwon, Gun-Hee ; Park, Jae-Yong ; Cha, Jae-Ho ; Song, Young-Sun ; Kim, Jeong-Hwan 2011. "Cloning and Overexpression of aprE3-17 Encoding the Major Fibrinolytic Protease of Bacillus licheniformis CH 3-17" Biotechnology and bioprocess engineering, 16(2): 352~359 

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