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Miniscale Identification and Characterization of Subtilisins from Bacillus sp. Strains 원문보기

Journal of microbiology and biotechnology, v.15 no.3, 2005년, pp.537 - 543  

CHOI NACK-SHICK (Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ,  JU SUNG-KYU (Department of Biology, Chungnam National University) ,  LEE TAE YOUNG (Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ,  YOON KAB-SEOG (Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ,  CHANG KYU-TAE (Primate Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ,  MAENG PIL JAE (Microbiology, Chungnam National University) ,  KIM SEUNG-HO (Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)

Abstract ▼ AI-Helper 아이콘AI-Helper

Subtilisin (EC 3.4.21.14) is the major extracellular alkaline serine protease of Bacillus species. Previously, we found that subtilisins did not migrate in the electrophoretic field in the Laemmili buffer system due to their high pI values (over 8.8); however, it formed a 'binding mode' at the top o...

주제어

#Subtilisin   #binding mode   #Bacillus   #over-running electrophoresis   #zymography  

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