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논문 상세정보


Saccharomyces yeast spores are more resistant to drying and storage than vegetative cells. For the mass production of yeast spores, compressed yeast was directly inoculated into a sporulation medium (SM). The effects of inoculum size and the addition of rice wine cake (RWC) into SM on the sporulation were examined using flasks. With $1\%$ inoculum of compressed yeast, $1.45{\times}10^8/ml$ of asci was obtained. The addition of $0.5\%$ RWC into SM improved the cell growth and spore yield, and the number of asci formed was $2.31{\times}10^8/ml$. The effects of culture temperature, temperature-shift, and concentrations of inoculum, potassium acetate, and RWC on the sporulation were also evaluated using a jar fermentor. The optimum temperature for spore formation was $22^{\circ}C$ where the number of asci formed was $2.46{\times}10^8/ml$. The shift of culture temperature from initial $30^{\circ}C$ for 1 day to $22^{\circ}C$ for 3 days increased the number of asci formed to $2.96{\times}10^8/ml$. The use of $2\%$ (w/v) inoculum of compressed yeast, $2\%$ potassium acetate, and $1\%$ (w/v) RWC in SM with the shift of culture temperature of initial $30^{\circ}C\;to\;22^{\circ}C$ resulted in $90\%$ sporulation ratio and formation of $6.18{\times}10^8\;asci/ml$.

참고문헌 (14)

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  9. Marmirol, N., M. Ferri, and P. P. Puglisi. 1983. Involvement of mitochondrial protein synthesis in sporulation: Effect of erythromycin on macromolecular synthesis, meiosis, and ascospore formation in Saccharomyces cerevisiae. J. Bacteriol. 154: 118-129 
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  13. Miller, J. J. 1989. Sporulation in Saccharomyces cerevisiae, pp. 489-541. In A. H. Rose and J. S. Harrison (eds.), The Yeasts, vol. 3. Academic Press, New York, U.S.A 
  14. Marmiroli, N. and T. Lodi. 1984. Modification of nuclear gene expression by inhibition of mitochondrial translation during sporulation in MAT${\alpha}$/MATa diploids of Saccharomyces cerevisiae. Mol. Gen. Genet. 198: 69-74 

이 논문을 인용한 문헌 (3)

  1. 2006. "" Journal of microbiology and biotechnology, 16(11): 1684~1689 
  2. 2006. "" Journal of microbiology and biotechnology, 16(7): 1041~1046 
  3. 2009. "" Journal of microbiology and biotechnology, 19(10): 1161~1168 


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