[논문]Purification and Characterization of Pyrimidine Nucleotide N-Ribosidase from Pseudomonas oleovorans

YU, Tae-Shick

Purification and Characterization of Pyrimidine Nucleotide N-Ribosidase from Pseudomonas oleovorans 원문보기

Journal of microbiology and biotechnology, v.15 no.3, 2005년, pp.573 - 578

YU, Tae-Shick (Department of Microbiology, Keimyung University)

Abstract ▼ AI-Helper

Pyrimidine nucleotide N-ribosidase (pyrimidine 5'-nucleotide phosphoribo(deoxyribo)hydrolase/pyrimidine 5'-nucleotide nucleosidase, EC 3.2.2.10) catalyzes the breakdown of pyrimidine 5'-nucleotide into pyrimidine base and ribose(deoxyribo)-5-phosphate. However, detailed characteristics of the enzyme have not yet been reported. The enzyme was purified to homogeneity 327.9-fold with an overall yield of $6.1\%$ from Pseudomonas oleovorans ATCC 8062. The enzyme catalyzed cytidine monophosphate (CMP) and uridine monophosphate (UMP), but not adenosine monophosphate (AMP) and guanosine monophosphate (GMP). The enzyme optimally metabolized CMP at pH 6.0 and UMP at around 8.5, and the optimum temperature for the overall enzyme reaction was found to be $37^{\circ}C$. The $K_m$ values of the enzyme for CMP (at pH 6.0) and UMP (at pH 8.5) were 1.6 mM and 1.1 mM, respectively. AMP, deoxyCMP, and deoxyUMP were very effective inhibitors of the reaction. Double-reciprocal plots obtained in the absence and in the presence of AMP revealed that this inhibitory effect was of the mixed competitive type with respect to the breakdown of CMP and of the noncompetitive type with respect to the breakdown of UMP. In the presence of AMP, the enzyme followed sigmoid kinetics with respect to each substrate.

주제어

참고문헌 (15)

Agarwal, D. P., G. G. Sanwal, and P. S. Krishnan. 1963. Improved paper-chromatographic separation of sugar phosphates by using borate-impregnated paper. Analyst 88: 969-970

상세보기
Davis, B. J. 1964. Disc electrophoresis. 2. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121: 404-427

상세보기
Imada, A., M. Kuno, and S. Igarasi. 1967. Degradation of pyrimidine nucleotides by enzyme systems of Streptomyces. I. Ribose-5-phosphate formation from pyrimidine nucleotides. J. Gen. Appl. Microbiol. 13: 255-265

상세보기
Imada, A. 1967. Degradation of pyrimidine nucleotides by enzyme systems of Streptomyces. II. Pyrimidine 5'-nucleotide phosphoribo(deoxyribo) hydrolase of Streptomyces virginiae. J. Gen. Appl. Microbiol. 13: 267-278

상세보기
Kim, J. and T. S. Yu. 2004. Purification and properties of intracellular cytosine deaminase from Chromobacterium violaceum YK 391. J. Microbiol. Biotechnol. 14: 1182-1189

원문보기 상세보기
Kim, D. Y., J. S. Nam, Y. H. Rhee, and Y. B. Kim. 2003. Biosynthesis of novel poly(3-hydroxyalkanoates) containing alkoxy groups by Pseudomonas oleovorans. J. Microbiol. Biotechnol. 13: 632-635

원문보기 상세보기
Lineweaver, H. and D. Burk. 1934. The determination of enzyme dissociation constants. J. Am. Chem. 56: 658-666

상세보기
Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275
Neuhard, J. 1968. Pyrimidine nucleotide methabolism and pathways of thymidine triphosphate biosynthesis in Salmonella typhimurium. J. Bacteriol. 96: 1519-1527
Sakai, T., T. Watanabe, and I. Chibata. 1968. Metabolism of pyrimidine nucleotides in bacteria. J. Ferm. Technol. 46: 202-213
Sakai, T., T. Watanabe, and I. Chibata. 1971. Metabolism of pyrimidine nucleotides in bacteria. (II) Studies on the regulation system of the degradation of nucleotides in Pseudomonas oleovorans. J. Ferm. Technol. 49: 488-498
Sakai, T., T. Watanabe, and I. Chibata. 1971. Metabolism of pyrimidine nucleotides in bacteria. (III) Enzymatic production of ribose-5-phosphate from uridine-5-monophosphate by Pseudomonas oleovorans. Appl. Microbiol. 22: 1085-1090

상세보기
Sakai, T., T. Yu, and S. Omata. 1976. Distribution of enzymes related to cytidine degradation in bacteria. Agric. Biol. Chem. 40: 1893-1895

상세보기
Somogyi, M. 1952. Notes on sugar determination. J. Biol. Chem. 195: 19-23
Yu, T. S. 2004. Optimization of culture conditions for the production of pyrimidine nucleotide N-ribosidase from Pseudomonas oleovarans. J. Life Sci. 14: 608-613

원문보기 상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증