[논문]Intracellular Responses of Antibody-Producing H69K-NGD Transfectoma Subjected to Hyperosmotic Pressure

Bae, Sung-Won; Lee, Gyun-Min

Intracellular Responses of Antibody-Producing H69K-NGD Transfectoma Subjected to Hyperosmotic Pressure 원문보기

Journal of microbiology and biotechnology, v.15 no.3, 2005년, pp.579 - 586

Bae, Sung-Won (Division of Cardiology, Samsung Medical Center & Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine) , Lee, Gyun-Min (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)

Abstract ▼ AI-Helper

When subjected to hyperosmotic pressure by NaCl addition, H69K-NGD transfectoma, like KR12H-2 transfectoma, displayed decreased specific growth rate (${\mu}$) and increased specific antibody productivity ($q_{Ab}$): Elevation of medium osmolality from 280 mOsm/kg to 415 mOsm/kg decreased ${\mu}$ by $79\%$ in batch cultures of H69K-NGD transfectoma, while it increased $q_{Ab}$ by $103\%$. However, unlike KR12H-2 tranfectoma, enhanced $q_{Ab}$ of H69K-NGD transfectoma at hyperosmolalities was not due to elevated levels of Ig mRNAs. In hyperosmotic cultures of H69K-NGD transfectoma, heavy-chain mRNA per cell was not enhanced with increasing osmolality. Hyperosmotic pressure was found to preferentially enhance immunoglobulin (Ig) translation rates of H69K-NGD transfectoma. However, under hyperosmotic pressure, the translation rate of Ig polypeptides was not enhanced as much as $q_{Ab}$. This result suggests that hyperosmotic pressure also influences the post-translational process. Taken together, the results obtained show that intracellular response of transfectomas to hyperosmotic pressure, in regard to the main intracellular steps of the antibody secretory pathway, is cell-line dependent.

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참고문헌 (22)

Burton, D. R. 1991. Hwnan and mouse antibodies by repertoire cloning. Trends Biotechnol. 9: 169-175

상세보기
Chung, J. Y, H. K. Ahn, S. W. Lim, Y. H. Sung, Y. W. Koh, S. K. Park, and G. M. Lee. 2003. Development of recombinant Chinese hamster ovary cell lines producing human thrombopoietin or its analog. J. Microbiol. Biotechnol. 13: 759- 766

원문보기 상세보기
Ducommun, P., P.-A. Ruffieux, U. von Stokar, and I. Marison. 2001. The role of vitamins and amino acids on hybridoma growth and monoclonal antibody production. Cytotechnology 37: 65-73

상세보기
Hong, H. J., A. K. Kim, C. J. Ryu, S. S. Park, H. K. Chung, K. S. Kwon, K. L. Kim, J. Kim, and M. H. Han. 1992. Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody specific for pre-S2 antigen of hepatitis B virus. Gene 121: 331-335

상세보기
Jin, B. R., C. J. Ryu, S. S. Park, U. Namgung, H. J. Hong, and M. H. Han. 1993. Cloning, expression and characterization of a murine-human chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus. Mol. lmmunol. 30: 1647-1654
Kwak, B.-Y, B.-J. Kwon, C.-H. Kweon, and D.-H. Shon. 2004. Detection of Aspergillus, Penicillium, and Fusarium species by sandwich enzyme-linked immunosorbent assay using mixed monoclonal antibodies. J. Microbiol. Biotechnol. 14: 385-389

원문보기 상세보기
Kim, N. S. and G. M. Lee. 2002. Response of Chinese hamster ovary cells to hyperosmotic pressure: Effect of Bcl-2 overexpression. J. Biotechnol. 95: 237-248

상세보기
Kim, N. S., K. H. Chang, B. S. Chung, S. H. Kim, J. H. Kim, and G. M. Lee. 2003. Characterization of humanized antibody produced by apoptosis-resistant CHO cells under sodium butyrate-induced condition. J. Microbiol. Biotechnol. 13: 926-936

원문보기 상세보기
Laemmli, U. K. 1970. Cleavage of structual proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685

상세보기
Lee, G. M., A. Varma, and B. O. Palsson. 1991. Production of monoclonal antibody using free-suspended and immobilized hybridoma cells: Effect of serum. Biotechnol. Bioeng. 38: 821-830

상세보기
Lee, M. S. and G. M. Lee. 2000. Hyperosmotic pressure enhances immunoglobulin transcription rates and secretion rates of KR12H-2 transfectoma. Biotechnol. Bioeng. 68: 260-268

상세보기
Marks, D. M. 2003. Equipment design considerations for large scale cell culture. Cytotechnology 42: 21-33

상세보기
Nuss, D. L. and G. Koch. 1976. Variation in the relative synthesis of immunoglobulin G and non-immunoglobulin G proteins in cultured MPC-11 cells with changes in the overall rate of polypeptide chain initiation and elongation. J. Mol. Biol. 102: 601-610

상세보기
Oh, S. K. W., P. Vig, F. Chua, W. K. Teo, and M. G. S. Yap. 1993. Substantial overproduction of antibodies by osmotic pressure and sodium butyrate. Biotechnol. Bioeng. 42: 601-610

상세보기
Oh, S. K. W., F. K. F. Chua, and A. B. H. Choo. 1995. Intracellular responses of productive hybridoma subjected to high osmotic pressure. Biotechnol. Bioeng. 46: 525-535

상세보기
Oyass, K., T. E. Ellingsen, N. Dryset, and D. W. Levine. 1994. Hyperosmotic hybridoma cell cultures: Increased monoclonal antibody production with addition of glycine betaine. Biotechnol. Bioeng. 44: 991-998

상세보기
Park, S. Y. and G. M. Lee. 1995. Enhancement of antibody productivity by immobilized hybridoma cell culture with hyperosmolar medium. Biotechnol. Bioeng. 48: 699-705

상세보기
Ryu, J. S., M. S. Lee, and G. M. Lee. 2001. Effects of cloned gene dosage on the response of recombinant CHO cells to hyperosmotic pressure in regard to cell growth and antibody production. Biotechnol. Prog. 17: 993-999

상세보기
Sambrook, J., E. F. Fritisch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, U.S.A
Dorner, A. J., L. C. Wasley, and R. J. Kaufman. 1989. Increased synthesis of secreted proteins induces expression of glucose-regulated proteins in butyrate-treated Chinese hamster ovary cells. J. Biol. Chem. 264: 20602-20607

상세보기
Parker, M. I., J. B. Haan, and W. Gevers. 1986. DNA hypermethylation in sodium butyrate-treated WI-38 fibroblasts. J. Biol. Chem. 261: 2786-2790
Stryer, L. 1995. Biochemistry. W. H. Freeman, New York, U.S.A

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