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"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
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논문 상세정보

Abstract

It is likely that maltose could provide a good substrate for the bacteria in the intestine, when the pathogenic bacteria invade and colonize in human gut. For better understanding of this organism's maltose metabolism, a mutant that was not able to grow with maltose as a sole carbon source was screened from a library of mutants constructed by a random transposon mutagenesis. By a transposon-tagging method, malPQ genes encoding a maltodextrin phosphorylase and a 4-${\alpha}$-glucanotransferase, were identified and cloned from Vibrio vulnificus. The deduced amino acid sequences of malPQ from V. vulnificus were 48 to $91\%$ similar to those of MalP and MalQ reported from other Enterobacteriaceae. Functions of malPQ genes were assessed by the construction of mutants whose malPQ genes were inactivated by allelic exchanges. When maltose was used as the sole carbon source, neither malP nor malQ mutant was able to grow to a substantial level, revealing that the MalP and MalQ are the only enzymes for metabolic utilization of maltose. The malQ mutant exhibited decreased adherence toward intestinal epithelial cells in vitro, but there was no difference in the $LD_{50}s$ of the wild-type and the malQ mutant in mice. Therefore, it appears that MalQ is less important in the pathogenesis of V. vulnificus than would have been predicted by considering maltose as a most common sugar in the intestine, but not completely dispensable for virulence in mice.

참고문헌 (26)

  1. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 
  2. DeLorenzo, V., M. Herrero, U. Jakubzik, and K. Timmis. 1990. Mini- Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J. Bacteriol. 172: 6568-6572 
  3. Kim, H. J., J. H. Lee, J. E. Rhee, H. S. Jeong, H. K. Choi, H. J. Chung, S. Ryu, and S. H. Choi. 2002. Identification and functional analysis of the putAP genes encoding Vibrio vulnificus proline dehydrogenase and proline permease. J. Microbiol. Biotechnol. 12: 318-326 
  4. Lang, H., G. Jonson, J. Holmgren, and E. T. PaIva. 1994. The maltose regulon of Vibrio cholerae affects production and secretion of virulence factors. Infect. Immun. 62: 4781-4788 
  5. Lee, J. H., N. Y. Park, S.-J. Park, and S. H. Choi. 2003. Identification and characterization of the Vibrio vulnificus phosphomannomutase gene. J. Microbiol. Biotechnol. 13: 149-154 
  6. Linkous, D. A. and J. D. Oliver. 1999. Pathogenesis of Vibrio vulnificus. FEMS Microbiol. Lett. 174: 207-214 
  7. Paranjpye R. N., J. C. Lara, J. C. Pepe, C. M. Pepe, and M. S. Strom. 1998. The type IV leader peptidase/N-methyltransferase of Vibrio vulnificus controls factors required for adherence to HEp-2 cells and virulence in ironoverloaded mice. Infect. Immun. 66: 5659-5668 
  8. Reed, L. J. and H. Muench. 1938. A simple method of estimating fifty percent endpoints. Am J. Hyg. 27: 439-497 
  9. Staskawicz, B., D. Dahlbeck, K. Keen, and C. Napoli. 1987. Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea. J. Bacteriol. 169: 5789-5794 
  10. Wright, A. C., L. M. Simpson, J. D. Oliver, and J. G. Morris, Jr. 1990. Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus. Infect. Immun. 58: 1769-1773 
  11. Xavier, K. B., R. Peist, M. Kossmann, W. Boos, and H. Santos. 1999. Maltose metabolism in the hyperthermophilic archaeon Thermococcus litoralis: Purification and characterization of key enzymes. J. Bacteriol. 181: 3358-3367 
  12. Boos, W. and H. Shuman. 1998. Maltose/maltodextrin system of Escherichia coli: Transport, metabolism, and regulation. Microbiol. Mol. Biol. Rev. 62: 204-229 
  13. Jeong, H. S., J. E. Rhee, J. H. Lee, H. K. Choi, D. I. Kim, M. H. Lee, S. J. Park, and S. H. Choi. 2003. Identification of lrp and its influence on survival under various stresses. J. Microbiol. Biotechnol. 13: 159-163 
  14. Oka, A., H. Sugisaki, and M. Takanami. 1981. Nucleotide sequence of the kanamycin resistance transposon Tn903. J. Mol. Biol. 147: 217-226 
  15. Boos, W. and J. M. Lucht. 1996. Periplasmic binding protein-dependent ABC transporter, pp. 1175-1209. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (eds.), Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd Ed. American Society for Microbiology, Washington, D.C., U.S.A 
  16. Lee, B. C., S. H. Choi, and T. S. Kim. 2004. Sulforhodamine B assay for determining cytotoxicity of Vibrio vulnificus against human intestinal cells. J. Microbiol. Biotechnol. 14: 350-355 
  17. Jeong, K. C., H. S. Jeong, J. H. Rhee, S. E. Lee, S. S. Chung, A. M. Starks, G. M. Escudero, P. A. Gulig, and S. H. Choi. 2000. Construction and phenotypic evaluation of a Vibrio vulnificus vvpE mutant for elastolytic protease. Infect Immun. 68: 5096-5106 
  18. Kim, J., J. G. Kim, B. K. Park, O. Choi, C. H. Park, and I. Hwang. 2003. Identification of genes for biosynthesis of antibacterial compound from Pseudomonas fluorescens B16, and its activity against Ralstonia solanacearum. J. Microbiol. Biotechnol. 13: 292-300 
  19. Miller, V. L. and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170: 2575-2583 
  20. Sambrook, J. and D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A 
  21. Keen, N. T., S. Tamaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria. Gene 70: 191-197 
  22. Rhee, J. E., J. H. Rhee, P. Y. Ryu, and S. H. Choi. 2002. Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress. FEMS Microbiol. Lett. 208: 245-251 
  23. Donnenberg, M. S. and J. B. Kaper. 1991. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59: 4310-4317 
  24. Jeong, H. S., K. C. Jeong, H. K. Choi, K. J. Park, K. H. Lee, J. H. Rhee, and S. H. Choi. 2001. Differential expression of Vibrio vulnificus elastase gene in a growth phase-dependent manner by two different types of promoters. J. Biol. Chem. 276: 13875-13880 
  25. Strom, M. S. and R. N. Paranjpye. 2000. Epidemiology and pathogenesis of Vibrio vulnificus. Microbes Infect. 2: 177-188 
  26. Rhee, J. E., J. H. Lee, H. S. Jeong, U. Park, D. H. Lee, G. J. Woo, S. I. Miyoshi, and S. H. Choi. 2003. Evidence that temporally alternative expression of the Vibrio vulnificus elastase prevents proteolytic inactivation of hemolysin. J. Microbiol. Biotechnol. 13: 1021-1026 

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