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논문 상세정보

An Efficient and Stable Method for the Transformation of Heterogeneous Genes into Cephalosporium acremonium Mediated by Agrobacterium tumefaciens

Abstract

A transformation system mediated by Agrobacterium tumefaciens is routinely used for the genetic engineering of plants. Here, we report an efficient and stable method for transformation of heterogeneous genes into an industrial Cephalosporium acremonium by using a similar transformation system established in plants. Both the phleomycin-resistant gene and vgb gene were used as screening markers to confirm the success of transformation by either Southern hybridization or PCR amplification. It was found that acetosyringone (AS) was necessary only for protoplast transformation and the heterogeneous genes transferred were integrated into the genome of C. acremonium. The transformation efficiency obtained with this system was much higher than the conventional techniques used for transformation of C. acremonium.

참고문헌 (16)

  1. Austin, B., R. M. Hall, and B. M. Tyler. 1990. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162 
  2. Bennett, J. W. and L. L. Lasure. 1991. Growth media, pp. 441-458, In: Bennett, J. W. and L. L. Lasure (eds.). More Gene Manipulations in Fungi. Academic Press, San Diego, CA, U.S.A 
  3. Isogai, T., M. Yoshida, M. Tanaka, and H. Aoki. 1987. Transformation of Acremonium chrysogenium and Saccharomyces cerevisiae using an antibiotic resistance marker Agric. Biol. Chem. 51: 2321-2329 
  4. Sambrook, J., F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A 
  5. Webster, D. A. and C. Y. Liu. 1974. Reduced nicotinamide adenine dinucleotide cytochrome o reductase associated with cytochrome o purified from Vitreoscilla. J. Biol. Chem. 249: 4259-4260 
  6. Hooykaas, P. J. J. and A. G. M. Beijersbergen. 1994. The virulence system of Agrobacterium tumefaciens. Annu. Rev. Phytopathol. 2: 157-179 
  7. Zeng, R., C. B. Zhu, W. J. Zhao, and B. Q. Zhu. 1999. Transformation of plasmid p YG715/vgb bearing TrpC promoter in Cephalosporium acremonium. Chinese J Antibiot. 24: 269-272 
  8. Skatrud, P. L., A. J. Teitz, T. D. Ingolia, C. A. Cantwell, D. L. Fisher, J. L. Chapman, and S. W. Queener. 1989. Use of recombinant DNA to improve production of cephalosporin C by C. acremonium. Bio/Technology 7: 477-485 
  9. Winans, A. C. 1992. Two way chemical signaling in Agrobacterium-plant interactions. Microbiol. Rev. 56: 12-31 
  10. Groot, M. J., P. Bundock, P. J. Hooykaas, and A. G. Beijersbergen. 1998. Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat. Biotechnol. 16: 839-842 
  11. Bundock, P., A. den Dulk-Ras, A. Beijershergen, and P. J. J. Hooykass. 1995. Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J 14: 3206-3214 
  12. Bevan, M. 1984. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 22: 8711-8721 
  13. Piers, K. L., J. D. Heath, X. Liang, K. M. Stephens, and E. W. Nester. 1996. Agrobacterium tumefaciens-mediated transformation of yeast. Proc. Natl. Acad. Sci. USA 93: 1613-1618 
  14. Raeder, U. and P. Broda. 1985. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1: 17-20 
  15. Mattanovish, D., F. Ruker, A. da C. Machado, M. Laimer, F. Regner, H. Steinkellner, G. Himmber, and H. Katinger. 1989. Efficient transformation of Agrobacterium spp. by electroporation. Nucleic Acids Res. 17: 6747 
  16. Hamilton, C. M., A. Frary, C. Lewis, and S. Tanksley. 1996. Stable transfer of intact high molecular weight DNA into plant chromosomes. Proc. Natl. Acad. Sci. USA 93: 9975-9979 

이 논문을 인용한 문헌 (3)

  1. 2006. "" Journal of microbiology and biotechnology, 16(8): 1236~1239 
  2. 2007. "" Journal of microbiology and biotechnology, 17(4): 547~559 
  3. 2008. "" Journal of microbiology and biotechnology, 18(2): 234~241 

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