$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

Cytosolic sialidase (Neu2), a member of the sialidase family that is responsible for hydrolysis of sialic acid from the terminal position of sialoglycoconjugates, is poorly expressed in skeletal muscle and not detected in any other adult tissues. Thus, we isolated Neu2 cDNA using splicing by overlap extension (SOEing). In order to further characterize this enzyme, a His-tagged derivative was expressed in the bacterial expression system and purified by $Ni^{2+}$-affinity chromatography. A recombinant product of approximately 42 kDa had sialidase activity toward 4-methyl-umbelliferyl-$\alpha$-D-N-acetylneuraminic acid (4MU-NeuAc). The optimal pH and temperature of the recombinant Neu2 for 4MU-NeuAc was 6.0 and $37.5^{\circ}C$, respectively. The metal ions, such as $Cu^{2+}\;and\;Cd^{2+}$, showed strong inhibitory effect on the activity of the enzyme. The enzyme efficiently hydrolyzed the gangliosides GM3 and GD3 and had relatively low activities on ganglioside GD1a and GD1b, $\alpha$2-3 sialyllactose, and sialylated glycoproteins such as fetuin, transferrin, and orsomucoid, but had hardly any activities on $\alpha$2-6 sialyllactose and ganglioside GM1 and GM2. We concluded that the recombinant Neu2 has a sialidase activity toward glycoproteins as well as gangliosides.

참고문헌 (31)

  1. Achyuthan, K. E. and A. M. Achyuthan. 2001 Comparative enzymology, biochemistry and pathophysiology of human exo-alpha-sialidases (neuraminidases). Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 129: 29-64 
  2. Chung, T. W, H. J. Choi, Y. C. Lee, and C. H. Kim. 2005. Molecular mechanism for transcriptional activation of ganglioside GM3 synthase and its function in differentiation of HL-60 cells. Glycobiology 15: 233-244 
  3. Ha, K. T., S. H. Cho, J. K. Kim, Y. C. Lee, and C. H. Kim. 2004. Molecular characterization of membrane type gangliosidespecific sialidase (Neu3) from K562 erythroleukemia cell. Mol. Cells 17: 267-273 
  4. Horton, R. M., H. D. Hunt, S. N. Ho, J. K. Pullen, and L. R. Pease. 1989. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension. Gene 77: 61-68 
  5. Horton, R. M., Z. L. Cai, S. N. Ho, and L. R. Pease. 1990. Gene splicing by overlap extension: Tailor-made genes using the polymerase chain reaction. Biotechniques 8: 528-535 
  6. Horton, R. M., S. N. Ho, J. K. Pullen, H. D. Hunt, Z. Cai, and L. R. Pease. 1993. Gene splicing by overlap extension. Methods Enzymol. 217: 270-279 
  7. Miyagi, T, K. Konno, Y. Emori, H. Kawasaki, K. Suzuki, A. Yasui, and S. Tsuiki. 1993. Molecular cloning and expression of cDNA encoding rat skeletal muscle cytosolic sialidase. J. Biol. Chem. 268: 26435-26440 
  8. Miyagi, T, J. Sagawa, K. Konno, and S. Tsuiki. 1990. Immunological discrimination of intralysosomal, cytosolic, and two membrane sialidases present in rat tissues. J. BioChem. (Tokyo) 107: 794-798 
  9. Miyagi, T, J. Sagawa, K. Konno, S. Hanada, and S. Tsuiki. 1990. Biochemical and immunological studies on two distinct ganglioside-hydrolyzing sialidases from the particulate fraction of rat brain. J. Biochem. (Tokyo) 107: 787-793 
  10. Monti, E., A. Preti, E. Rossi, A. Ballabio, and G Borsani. 1999. Cloning and characterization of NEU2, a human gene homologous to rodent soluble sialidases. Genomics 57: 137-143 
  11. Monti, E., A. Preti, B. Venerando, and G. Borsani. 2002. Recent development in mammalian sialidase molecular biology. Neur. Chem. Res. 27: 649-663 
  12. Saito, M., C. L. Fronda, and R. K. Yu. 1996. Sialidase activity in nuclear membranes of rat brain. J. Neurochem. 66: 2205-2208 
  13. Warren, L. 1959. The thiobarbituric acid assay of sialic acids. J. Biol. Chem. 234: 1971-1975 
  14. Traving, C. and R. Schauer. 1998. Structure, function and metabolism of sialic acids. Cell. Mol. Life Sci. 54: 1330-1349 
  15. Venerando, B., A. Fiorilli, L. Di Francesco, A. Chiarini, E. Monti, D. Zizioli, and G Tettamanti. 1994. Cytosolic sialidase from pig brain: A 'protein complex' containing catalytic and protective units. Biochim. Biophys. Acta 1208: 229-237 
  16. Corfield, T. 1992. Bacterial sialidases: Roles in pathogenicity and nutrition. Glycobiology 2: 509-521 
  17. Fronda, C. L., G. Zeng, L. Gao, and R. K. Yu. 1999. Molecular cloning and expression of mouse brain sialidase. Biochem. Biophys. Res. Commun. 258: 727 - 731 
  18. Hasegawa, T., C. Feijoo Camero, T. Wada, Y. !toyama, and T. Miyagi. 2001. Differential expression of three sialidase genes in rat development. Biochem. Biophys. Res. Commun. 280: 726-732 
  19. Kim, T. W, J. Y. Lee, H. S. Song, J. H. Park, G. E. Ji, and H. Y. Kim. 2004. Isolation and identification of Weissella kirnchii from green onion by cell protein pattern analysis. J. Microbiol. Biotechnol. 14: 105 - 109 
  20. Boguski, M. S. and G. D. Schuler. 1995. Establishing a human transcript map. Nat. Genet. 10: 369-373 
  21. Kim, M. K., Y. H. Cho, J. M. Kim, M. W Chun, S. K. Lee, Y. H. Lim, and C. H. Lee. 2003. Inhibition of cell-cycle progression in human promyelocytic leukemia HL-60 cells by MCS-C2, a novel cyclin-dependent kinase inhibitor. J. Microbiol. Biotechnol. 13: 607-612 
  22. Crennell, S. J., E. F. Garman, W G. Laver, E. R. Vimr, and G. L. Taylor. 1993. Crystal structure of a bacterial sialidase (trom Salmonella typhimurium LT2) shows the same fold as an influenza virus neuraminidase. Proc. Natl. Acad. Sci. USA 90: 9852-9856 
  23. Tringali, C., N. Papini, P. Fusi, G. Croci, G Borsani, A. Preti, P. Tortora, G. Tettamanti, B. Venerando, and E. Monti. 2004. Properties of recombinant human cytosolic sialidase HsNEU2. The enzyme hydrolyzes monomerically dispersed GM I ganglioside molecules. J. Biol. Chem. 279: 3169- 3179 
  24. Milner, C. M., S. V. Smith, M. B. Carrillo, G L. Taylor, M. Hollinshead, and R. D. Campbell. 1997. Identification of a sialidase encoded in the human major histocompatibility complex. J. Biol. Chem. 272: 4549-4558 
  25. Monti, E., M. T. Bassi, R. Bresciani, S. Civini, G. L. Croci, N. Papini, M. Riboni, G. Zanchetti, A. Ballabio, A. Preti, G. Tettamanti, B. Venerando, and G. Borsani. 2004. Molecular cloning and characterization of NEU4, the fourth member of the human sialidase gene family. Genomics 83: 445-453 
  26. Ferrari, J., R. Harris, and T. G. Warner. 1994. Cloning and expression of a soluble sialidase trom Chinese hamster ovary cells: Sequence alignment similarities to bacterial sialidases. Glycobiology 4: 367 - 373 
  27. Kim, C. H. 2004. Glycoantigen biosynthesis of human hepatoma and colon cancer cells are directly dependent on different N-acetylglucosaminyltransferase-III and -v activities. J. Microbiol. Biotechnol. 14: 891-900 
  28. Chung, T. W, U. H. Jin, and C. H. Kim. 2003. Salmonella typhimurium LPS confers its resistance to antibacterial agents ofbaicalin of Scutellaria baicalensis George and Novomycin: Complementation of the rfaE gene required for ADP-Lglycero-D-manno-heptose biosynthesis of lipopolysaccharide. J. Microbiol. Biotechnol. 13: 564-570 
  29. Schauer, R. 2000. Achievements and challenges of sialic acid research. Glycoconj. J. 17: 485-499 
  30. Monti, E., A. Preti, C. Nesti, A. Ballabio, and G. Borsani. 1999. Expression of a' novel human sialidase encoded by the Neu2 gene. Glycobiology 9: 1313-1321 
  31. Miyagi, T and S. Tsuiki. 1985. Purification and characterization of cytosolic sialidase from rat liver. J. Biol. Chem. 260: 6710-6716 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

원문보기

원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일