The purpose of this study was to characterize the putative anxiolytic-like effects of the aqueous extract of the root of Polygala tenuifolia ( AEPT) using an elevated plus maze (EPM) and hole-board apparatus in mice. The AFPT was orally administered at 50, 100, 200 or 400 mg/kg to ICR mice, 1 h bef...
The purpose of this study was to characterize the putative anxiolytic-like effects of the aqueous extract of the root of Polygala tenuifolia ( AEPT) using an elevated plus maze (EPM) and hole-board apparatus in mice. The AFPT was orally administered at 50, 100, 200 or 400 mg/kg to ICR mice, 1 h before the behavioral evaluation in the EPM respectively. Control mice were treated with an equal volume of saline, and positive control mice with buspirone (2 mg/kg). Single treatments of the AEPT significantly increased the percentage of time spent and arm entries into the open arms of the EPM vedrsus saline controls (P<0.05). Moreover, there were no changes in the locomotor activity and myorelaxant effects in any group compared with the saline controls. In the hole-board test,single treatments of the AEPT (200 and 400 mg/kg) significantly increased the number of headdips versus saline controls (P<0.05). In addition, the anxiolytic-like effects of the AEPT were blocked by WAY 100635(0.3mg/kg, I.p), a5-$HT_{1A}$ receptor antagonist not by flumazenil, a $GABA_{A}$ antagonist. These results indicate that P. tenuifolia is an effective anxiolytic agent, andsuggest that the anxiolytic-like effects of P. tenuifolia is mediated via the serotonergic nervous system.
The purpose of this study was to characterize the putative anxiolytic-like effects of the aqueous extract of the root of Polygala tenuifolia ( AEPT) using an elevated plus maze (EPM) and hole-board apparatus in mice. The AFPT was orally administered at 50, 100, 200 or 400 mg/kg to ICR mice, 1 h before the behavioral evaluation in the EPM respectively. Control mice were treated with an equal volume of saline, and positive control mice with buspirone (2 mg/kg). Single treatments of the AEPT significantly increased the percentage of time spent and arm entries into the open arms of the EPM vedrsus saline controls (P<0.05). Moreover, there were no changes in the locomotor activity and myorelaxant effects in any group compared with the saline controls. In the hole-board test,single treatments of the AEPT (200 and 400 mg/kg) significantly increased the number of headdips versus saline controls (P<0.05). In addition, the anxiolytic-like effects of the AEPT were blocked by WAY 100635(0.3mg/kg, I.p), a5-$HT_{1A}$ receptor antagonist not by flumazenil, a $GABA_{A}$ antagonist. These results indicate that P. tenuifolia is an effective anxiolytic agent, andsuggest that the anxiolytic-like effects of P. tenuifolia is mediated via the serotonergic nervous system.
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문제 정의
The purpose of this study was to characterize the anxiolyticlike activity of the aqueous extract of P. tenuifolia (AEPT). Its anxiolytic effects were examined using the elevated plus-maze (EPM) and the hole-board apparatus in mice, respectively.
제안 방법
And we examined the myorelaxant effects using a horizontal wire test in mice. In addition, this study also investigated which nervous systems are involved in the anxiolytic-like effects of the AEPT through the co-administration of AEPT and either fluma- zenilorWAY 100635.
The EPM is considered to be an etiologically valid animal model of anxiety because it uses natural stimuli, such as a fear of a new, brightly lit open space and a fear of balancing on a relatively narrow raised surface (Dawson and Tricklebank, 1995). In this study, the buspirone treatment prolonged the percentage of time spent in the open arms and the percentage of open arm entries (Fig. 1). The AEPT treatment also prolonged the percentage of time spent in the open arms as well as the percentage of open arm entries without altering the spontaneous behavior at the chosen dose regimen.
One hour after the AEPT treatment (50, 100, 200 and 400 ㎎/㎏, p.o.), the mice were placed in the EPM. The mice in the control group were given the vehicle solvent only, and the animals were tested individually once only for 5 min.
The maze was cleaned after each trial so as to remove any residue or odors. The following measurements were taken and analyzed using the video-based Ethovision System: the number of entries into the open or closed arms, the time spent in each arm, and the total distance moved in the EPM. All the experiments were carried out between 10:00 and 16:00 o'clock.
Testing was carried out in clear black Plexiglas boxes (40 x 40 x 40 cm) equipped with the video-based Ethovision System (Noldus, Wegeningen, The Netherlands). The mice were placed in the center of the apparatus to evaluate horizontal locomotor activity 1 h after being treated with AEPT (50, 100, 200, and 400 ㎎/㎏) and video-recorded for 5 min. The horizontal locomotor activity is expressed in terms of the total ambulatory distance and the hequency of rearing.
대상 데이터
Testing was carried out in clear black Plexiglas boxes (40 x 40 x 40 cm) equipped with the video-based Ethovision System (Noldus, Wegeningen, The Netherlands). The mice were placed in the center of the apparatus to evaluate horizontal locomotor activity 1 h after being treated with AEPT (50, 100, 200, and 400 ㎎/㎏) and video-recorded for 5 min.
The EPM for mice consisted of two perpendicular open arms (30 x 7 cm) and two enclosed arms (30 x 7 cm) with 20 cm high walls, extending from the central platform (7x7 cm). The open and closed arms were connected by a central square, 7x7 cm, to give an apparatus of a plus sign appearance.
The hole-board apparatus (Ugo Basile, Italy) consisted of gray Perspex panels (40 x 40 cm, 2.2 cm thick) with 16 equidistant holes 3 cm in diameter in the floor. Photocells below the surface of the holes measured the number of head-dips.
tenuifolia were obtained from a herbal supplier in Seoul, Korea, and voucher specimens (KHUOPS-04-31) were deposited at the herbarium of the College of Pharmacy, Kyung Hee University (Seoul, Korea). The material was authenticated by Prof. C.S. Yook of the Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University. All the other materials were of the highest grade commercially available.
(USA). The roots of P. tenuifolia were obtained from a herbal supplier in Seoul, Korea, and voucher specimens (KHUOPS-04-31) were deposited at the herbarium of the College of Pharmacy, Kyung Hee University (Seoul, Korea). The material was authenticated by Prof.
데이터처리
of 10- 12 mice. P values for group comparisons were obtained by one way ANOVA followed by Student Newman-Keuls test (*P< 0.05 versus the saline- treated control).
of 10- 12 mice. P values for group comparisons were obtained by one way ANOVA followed by Student Newman-Keuls test (*P< 0.05 versus the saline- treated control).
), or saline; N = 10-12 mice per group. P values for the group comparisons were obtained by one way ANOVA followed by Student Newman-Keuls test (*P<0.05 versus the saline-treated control, #P<0.05 as compared with the AEPT-treated group).
of 10-12 mice. P values for the group comparisons were obtained by one way ANOVA followed by Student-Newman-Keuls test (P < 0.05 as compared with the saline-treated control group).
The values are expressed as means 士 S.E.M. The data was analyzed by a one-way analysis of variance (ANOVA) followed by Student-Newman-Keuls test for the multiple comparisons. Statistical significance was set at P< 0.
성능/효과
However, the nature of its underlying mode of action remains to be elucidated. Although the findings of herb effects may not in general provide clinically useful outcomes in patients or in normal humans, the findings of this study may be important because they confirm the validity of P. tenuifolia as a medicinal plant.
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