$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

In the previous studies of Saccharomyces cerevisiae, Abp140p (actin binding protein 140) fused to GFP has been only a protein that can label actin cables of yeast cells so far. However, the role of Abp140p in actin dynamics was remained elusive. In this study, the function of Abp140p was investigated with a deletion mutant and overexpression of GFP fused Abp140p. The deletion mutant was slightly more susceptible to Latrunculin-A (Lat-A), an actin-monomer sequestering agent, than wild type, although no significant deformation of actin structures was caused by ABP 140 deletion. Overexpression of Abp140p-GFP retarded cell growth, and produced thick and robust actin cables. Lat-A was not able to destabilize the thick actin cables, which suggests that actin dynamics was compromised in the cells with surplus of Abp140p. Therefore, Abp140p seems to stabilize actin cables together with other bundling proteins. Recently, actin cable dynamics of budding yeast was found to have a resemblance to that of filopodial tip of cultured mammalian cells. Retrograde movement of actin cables from buds to mother cells indicated local generation of the cable at bud sites. By using Abp140p-GFP, we traced the steps in the generation of a new actin cable after elimination of old cables by sodium azide. Before the appearance of a new actin cable, Abp140p-GFP concentrated in buds and disappeared, as mother cells became abundant in actin cables. Our observations provide a direct evidence of actin cable formation at buds of budding cells.

참고문헌 (19)

  1. Asakura T., Sasaki T., Nagano F., Satoh A, Obaishi H., Nishioka H., Imamura H., Horta K., Tanaka K., Nakanishi H. and Takai Y.: Isolation and characterization of a novel actin filament-binding protein from Saccharomyces cerevisiae. Oncogene 16:121-130, 1998 
  2. Evangelista M., Pruyne D., Amberg D,C., Boone C. and Bretscher A.: Formins direct Arp2/3-independent actin filament assembly to polarize cell grewth in yeast. Nat. Cell Biol. 4:260-269, 2002 
  3. Waddle J.A., Karpova T.S., Waterston R.H. and Cooper J.A.: Movement of cortical actin patches in yeast. J. Cell Biol. 132:861-870, 1996 
  4. Wang W.S., Motes C.M., Mohamalawari D.R. and Blancalflor E.B.: Green fluorescent protein fusions to Arabidopsis fimbrin 1 for spatio-temporal imaging of F-actin dynamics in roots. Cell Motil. Cytoskeleton 59:79-93, 2004 
  5. Ito H., Fukuda Y., Murata K. and Kimura A.: Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168, 1983 
  6. Mallavarapu A and Mitchison T.: Regulated actin cytoskeleton assembly at filopodium tips controls their extension and retraction. J. Cell Biol. 146: 1097-1106, 1999 
  7. Paavilainen V.O., Bertling E., Falck S. and Lappalainen P.: Regulation of cytoskeletal dynamics by actin-monomer-binding proteins. Trends Cell Biol. 14:386-394, 2004 
  8. Longtine M.S., McKenzie A 3rd, Demarini D.J., Shah N.G., Wach A., Brachat A., Philippsen P. and Pringle J.R.: Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14:953-961, 1998 
  9. Gourlay C.W, Carpp L.N., Timpson P., Winder S.J. and Ayscough K.R.: A role for the actin cytoskeleton in cell death and aging in yeast. J. Cell Biol. 164:803-809, 2004 
  10. Pollard T.D.: Rate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments. J. Cell Biol. 103: 2747-2754, 1986 
  11. Noguchi T. and Miller K.G.: A role for actin dynamics in individualization during spermatogenesis in Drosophila melanogaster. Development 130:1805-1816, 2003 
  12. Boldogh I.R., Yang H.C., Nowakowski WD., Karmon S.L., Hays L.G., Yates J.R. 3rd and Pon L.A.: Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast. Proc. Natl. Acad. Sci. USA 98:3162-3167, 2001 
  13. Yang H.C. and Pon L.A.: Actin dynamics in budding yeast. Proc. Natl. Acad. Sci. USA 99:751-756, 2002 
  14. Huckaba T.M., Gay A.C., Pantalena L.F., Yang H.C. and Pon L.A.: Live cell imaging of the assembly, disassembly, and actin cable-dependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae. J. Cell Biol. 167:519-530, 2004 
  15. Shelden E.A., Weinberg J.M., Sorenson D.R., Edwards C.A. and Pollock F.M.: Site-specific alteration of actin assembly visualized in living renal epithelial cells during ATP depletion. J. Am. Soc. Nephrol. 13:2667-2680, 2002 
  16. Atkinson S.J., Hosford M.A. and Molitoris B.A.: Mechanism of actin polymerization in cellular ATP depletion. J. Biol. Chem. 279:5194-5199, 2004 
  17. Ponti A., Machacek M., Gupton S.L., Waterman-Storer C.M. and Danuser G.: Two distinct actin networks drive the protrusion of migrating cells. Science 305: 1782-1786, 2004 
  18. Adams A.E. and Pringle J.R.: Relationship of actin and tubulin distribution to bud growth in wild-type and morphogeneticmutant Saccharomyces cerevisiae. J. Cell Biol. 98:934-945, 1984 
  19. Fehrenbacher K.L., Yang H.C., Gay A.C., Huckaba T.M. and Pon L.A.: Live cell imaging of mitochondrial movement along actin cables in budding yeast. Curr. Biol. 14: 1996-2004, 2004 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

원문보기

원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일