Flavonoid Compounds from Viola hondoensis and Their Effect on Matrix Metalloproteinase-1 in Ultraviolet Irradiation of Cultured Human Skin Fibroblasts원문보기
Moon, Hyung-In
(Department of Dermatology, Seoul National University College of Medicine, and Clinical research Institute, Seoul National University Hospital)
,
Lee, Joong-Ku
(Korea Research Institute of Bioscience and Biotechnology)
,
Zee, Ok-Pyo
(College of Pharmacy, Sung kyun Kwan University)
,
Chung, Jin-Ho
(Department of Dermatology, Seoul National University College of Medicine, and Clinical research Institute, Seoul National University Hospital)
Although many studies have been performed to elucidate the molecular consequences of ultraviolet irradiation, little is known about the effect of natural products. Ultraviolet irradiation is widely considered to be an environmental stress. Here we investigated the effect of 2',4',7-trihydroxyisoflav...
Although many studies have been performed to elucidate the molecular consequences of ultraviolet irradiation, little is known about the effect of natural products. Ultraviolet irradiation is widely considered to be an environmental stress. Here we investigated the effect of 2',4',7-trihydroxyisoflavone on the regulation of MMP-1 and type 1 procollagen in Ultraviolet irradiation of cultured human dermal fibroblasts. Phytochemical investigation of the whole plants of Viola hondoensis led to the isolation of five flavonoids. The structures of these compounds were identified 2',4',5,7-tetrahydroxyisoflavone (1), 2',4',7-trihydroxyisoflavone (2), 4',7-dihydroxyflavone (3), isoorientin (4), and isovitexin (5) using spectroscopic analysis. Among these, 2',4',7-trihydroxyisoflavone reduced the expression of MMP-1 at the protein levels in a dose-dependent manner by ultraviolet irradiation. Taken together, our results suggest that 2',4',7-trihydroxyisoflavone an important role in the reduction of MMP-1 induction by ultraviolet irradiation.
Although many studies have been performed to elucidate the molecular consequences of ultraviolet irradiation, little is known about the effect of natural products. Ultraviolet irradiation is widely considered to be an environmental stress. Here we investigated the effect of 2',4',7-trihydroxyisoflavone on the regulation of MMP-1 and type 1 procollagen in Ultraviolet irradiation of cultured human dermal fibroblasts. Phytochemical investigation of the whole plants of Viola hondoensis led to the isolation of five flavonoids. The structures of these compounds were identified 2',4',5,7-tetrahydroxyisoflavone (1), 2',4',7-trihydroxyisoflavone (2), 4',7-dihydroxyflavone (3), isoorientin (4), and isovitexin (5) using spectroscopic analysis. Among these, 2',4',7-trihydroxyisoflavone reduced the expression of MMP-1 at the protein levels in a dose-dependent manner by ultraviolet irradiation. Taken together, our results suggest that 2',4',7-trihydroxyisoflavone an important role in the reduction of MMP-1 induction by ultraviolet irradiation.
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제안 방법
TLC was carried out on silica gel 60 F254 and RP-18 F254 plates (Merck, Germany). Column chromatography was performed over silica gel 60 (Merck, particle size 230-400 mesh, Germany) and Sephadex LH-20 (Pharmacia, Sweden).
대상 데이터
Elmer 1710 spectrophotometer. The NMR spectra were taken on a JEOL LA 300 ('H, 300 MHz; 13C, 75 MHz, Japan), a Broker GPX 400 ('H, 400 MHz; 13C, 100 MHz, Germany), and a Bruker AMX 500 (!H, 500 MHz; 13C, 125 MHz, Germany) spectrometer. EIMS spectra were obtained on a VG Trio-2 spectrometer(USA).
The results were expressed as a percentage of control in six cultures. The UV light source was a F75/85W/UV21 fluorescent sun lamps, having an emission spectrum between 285-350 nm (peec at 310-315 nm) as previously described (7). A Kodacel filter (TA401/407; Kodak, Rochester, NY) was mounted 2 cm in front of the UV tubes to remove wavelengths <290 nm (UV-C).
데이터처리
, Boston, MA, USA) were used as primary antibodies. Statistical significance was determined using the Student t-tests. Results are presented by means ± standard error of mean(SEM).
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