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논문 상세정보

Isolation and Identification of Antimicrobial Substance from Canavalia gladiata

Abstract

Novel antimicrobial substance was isolated from seed coat of Canavalia gladiata by extraction with 75% methanol. Isolation and purification were conducted with solvent fractionation and chromatography on silica gel and sephadex LH-20 columns. Each fraction of antimicrobial activity was tested by paper disc method. Single compound was obtained from the 4th fraction of sephadex LH-20 column chromatography using chloroform/methanol (1:4, v/v), and identified as 3,4,5-trihydroxybenzoic acid methyl ester (methyl gallate) based on HPLC, GC/MS, FT-IR, $^1H$ NMR, and $^{13}C$ NMR analyses. This is the first report describing the presence of methyl gallate in C. gladiata.

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참고문헌 (15)

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이 논문을 인용한 문헌 (3)

  1. 2009. "" 목재공학 = Journal of the Korean wood science and technology, 37(2): 164~170 
  2. Kim, Un-Sung ; Kim, Jae-Young ; Kim, Seong-Jo ; Moon, Kwang-Hyun ; Baek, Seung-Hwa 2012. "Isoflavonoid Contents, Antibacterial Activities, and Physiological Activities of Cheonggukjang Made from Sword Bean" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 41(2): 174~181 
  3. Lee, Han-Na ; Kim, Jin-Kyu ; Kwon, Gyoo-Taik ; Shim, Jae-Hoon ; Kim, Jong-Dai ; YoonPark, Jung-Han 2012. "Anti-inflammatory Effects of Ethanol Extract from Bark of Acer barbinerve Maxim" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 41(9): 1242~1247 

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